Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

甘蒙北山—东疆天山元古代古陆壳特征及其早古生代地壳类型

本文研究了甘蒙北山及东疆天山早古生代地壳类型,划分为元古代的古陆壳、早古生代洋壳、拉张型过渡壳、汇聚型过渡壳。早古生代沿着库米什—卡瓦布拉克—星星峡—小黄山一线自震旦世晚期由东向西逐渐开裂,于晚寒武世至早奥陶世全面打开,形成库米什—小黄山洋盆。其北侧由塔里木古板块北缘分离出中天山微大陆。且末断裂带斜贯全区,起了控制东西两侧构造发展的转换断层作用。甘蒙北山较东疆天山的地壳类型更为复杂,先后发育有奥陶纪拉张型过渡壳—裂谷带和志留纪汇聚型过渡壳—沟弧盆体系。晚古生代全区构造演化进入开合交替时期,其开时形成拉张过渡壳,早古生代洋区在库鲁克塔格北缘哈孜布拉克塔格地区延续到泥盆—石炭纪。     

“将古论今”——环境科学研究中的历史地质学方法

传统地质学的“将今论古”方法使地质学取得了巨大的成就,而且还将是今后地质学发展的指导思想,但面对资源、环境、减灾等人类在二十一世纪的主要问题,地球科学所面对的问题时,地质学家首先应该改变或者更新自己的思维方式。如果以地球演化的同一性原理来认识地球,则“将古论今”的方法对解决地球科学面临的挑战人有重要的意义。以历史地质学的方法和视角,研究地质过程的发生、发展和演化过程,以及这种过程在今天和明天的演化趋势,及其对人类生存环境的影响,将是今后一十时期地质科学的主要任务。     

东阿拉伯海拉克希米盆地浊流沉积序列的粒度变化及其对中更新世气候转型的响应

阿拉伯海印度沉积扇是世界第二大海底扇,保存着来自喜马拉雅和喀喇昆仑等山系的新生代风化沉积物,是研究印度季风和南亚地区环境演化的理想材料。通过对2015年3~5月完成的IODP 355航次U1456钻孔A段总长345.36 m沉积物（约1.8 Ma以来）的沉积特征分析,发现其为典型的浊流沉积。通过对757个样品高分辨率的粒度测试,揭示研究区沉积序列在构造时间尺度上的粒度变化主要受到印度季风降水控制,阿拉伯海深海沉积物的粒度变化可作为一个新的季风演化的替代指标。从1.80~1.18 Ma到1.18~0.10 Ma,浊流沉积物的堆积速率降低,粒度变细,揭示了更新世印度季风强度的阶段性减弱。在沉积序列上,砂组分的变化周期从约41 ka向更长周期转化,推测东阿拉伯海U1456A钻孔沉积物序列在中更新世的粒度变化可能是对印度季风气候转型的响应。

     

Tectonics of Precambrian basement of the Tarim craton

The Altyn Tagh Mountain is the main area where the Precambrian basements of Tarim craton are exposed. There are two ophiolitic belts in Altyn Tagh: one belt is exposed in the northern margin of Altyn Tagh whose formation age is about (829±60) Ma, the other is situated along the southern margin of Altyn Tagh and has a formation age of about (1449±270) Ma. This paper proposes a simple tectonic model for the Precambrian basement of Tarim craton established from ophiolites in Altyn Tagh area. The south Tarim block had amalgamated with Qaidam block during about 1400-1500 Ma along the present Altyn fault, while the south Tarim-Qaidam united block was still separated from the north Tarim block by an ocean. The united block of south Tarim and Qaidam collided with north Tarim block along the zone of high positive anomaly of central Tarim, Hongliugou and Lapeiquan in about 800 Ma. So since the Sinian (beginning at 800 Ma) there has been an integrated basement for Tarim craton.     

新疆东准噶尔塔克扎勒—麦钦乌拉古缝合线的确定

本文通过构造分析及岩石学、地球化学研究,证实塔克扎勒—麦钦乌拉存在一条蛇绿混杂岩带,表现为在构造变形的砂泥质基质中分布若干个洋脊型和洋岛型的蛇绿岩碎块。该带两侧地壳厚度明显不同,大陆边缘增生方向相反,该带还是一条早古生代的生物群落界线,早石炭世两侧岛弧有较大的纬度差异(20°以上)。因此,塔克扎勒—麦钦乌拉蛇绿混杂岩带是古准噶尔大洋闭合的残骸,代表了西伯利亚板块和塔里木板块碰撞的缝合线构造带。     

Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

准噶尔盆地南缘中-新生界碎屑成份特征与构造期次

准噶尔盆地南缘晚侏罗世-早白垩世早期、晚白垩世及晚新生代发育的近源粗碎屑沉积显示构造活动的存在。野外剖面及镜下碎屑成份统计表明:砾岩的砾石成份、砂岩碎屑成份的物源属性主要是再旋回造山带和晚古生代的岩浆弧,但盆地南缘东段与西段的岩屑组成及物源属性存在较大的差异。其中,沉积岩岩屑在晚侏罗世—早白垩世早期、晚白垩世和晚新生代发生了相应的增加,显示盆缘沉积岩物源的隆升—剥蚀作用和构造活动的相对活跃。砂岩碎屑特征、重矿物相对含量及重矿物组合特征证明盆地南缘东、西两段的物源属性存在较大差异,特别是不稳定重矿物的增加显示晚侏罗世-早白垩世早期、晚白垩世和晚新生代为构造相对活跃的构造环境。综合中-新生界沉积碎屑特征及差异分析,准噶尔盆地南缘中-新生代盆山格局发生了3次较大的转变过程,分别对应于上述3个时期。中-新生代3次构造活动对含油气系统形成具有重要控制作用,构造活动期次与油气藏形成、调整的期次也有良好的对应关系。     

新疆库鲁克塔格斜长角闪岩岩石地球化学特征及其地质意义

库鲁克塔格地区位于塔里木盆地东北缘,在片麻岩中出露较多斜长角闪岩类包体,其走向大致与片麻理的方向一致。通过岩石化学、稀土元素地球化学和Ｓｍ－Ｎｄ同位素研究,确定该斜长角闪岩的原岩为基性火山岩,并形成于两种构造环境。北部托格地区斜长角闪岩球粒陨石标准化配分曲线呈近平坦型〔（Ｌａ／Ｙｂ）Ｎ＝０．６６—４．２３,一般在０．９１—１．６９之间;δＥｕ＝０．８２—１．１５,平均值为１．０２〕,与洋脊玄武岩类非常相似;南部阔克苏地区斜长角闪岩的球粒陨石标准化配分曲线显示出ＬＲＥＥ明显富集〔（Ｌａ／Ｙｂ）Ｎ＝２．７１—１０．８４;δＥｕ＝０．８１—１．０７,平均值为０．９５〕,具有类似现代岛弧或弧后盆地火山岩特有的稀土元素组成特征。它们的原岩岩浆来自长期亏损的地幔,基本未受到大陆地壳物质混染。     

GPS控制网起算点个数与分布的探讨与分析

GPS定位以其高精度、全天候、操作简便、观测时间短等优点广泛应用于各种测量控制网的建立。在提出GPS控制网起算点的要求的基础上,通过两个关联的实例验证GPS控制网起算点的个数及其分布的优化方案,得出一些有益的结论,对相关的生产实践工作具有一定的借鉴作用。