蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

平阳矾山明矾石矿床岩石学特征及其成因浅析

在野外地质工作的基础上，通过对浙江平阳研山明矾石矿床的岩石学及地球化学特征进行认真的研究，进一步证实了矾山明矾山矿床是酸性火山气热液与中酸性火山碎屑岩内的碱性长石的交代中形成的。为了解释矿床的均一性，成矿的周期性和矿床单向依变分带等重要特征，我们重点研究了矿床中明矾石化和叶蜡石化的热力学性质，得出了K 活度和pH值是控制交代产物的重要因素的新结论，提出了该矿床交代成矿作用的新模型，并据此成功地解释了矿床的一些重要特征。     

滇西北和邻区均衡重力异常特征及其壳内构造

本文以爱黎-海斯卡宁均衡补偿假设模式为基础,利用圆模板编制了滇西北及邻区均衡重力异常图。分析了异常的基本特征与壳内构造的关系,并初步探讨了均衡重力异常和强震活动的相关性。     

重力插值方法研究

为了根据离散观测数据构制连续空间重力变化图像，分析和讨论了３种数值插值方法，计算结果表明多面函数方法插值精度最高。由于逐步回归分析筛选核函数中心点的计算繁琐，文中提出根据分形理论和Ｓｈａｎｎｏｎ取样定理来确定核函数中心点。对滇西试验场进行模拟试算，插值精度可达到４～５（１０－８ｍｓ－２）。     

Profiles of spectral lines of an accretion ring around a Kerr black hole

Profiles of spectral lines emitted from an accretion ring around an object with strong gravitational field should be affected by Doppler shift, gravitational redshift, and deflection of light. Taking these effects into account, precise line profile of a Keplerian ring around a Kerr black hole for a distant observer is obtained by solving the kinetic equation of photons.     

VPN技术在浙江省气象网络中的应用

随着浙江省气象业务的不断发展，网络数据交换日益增多，同时更多基于网络的气象业务的增加，如网络视频会商、自动站数据传输等，使目前浙江省气象系统专线网络运行已达到极限，所以需要建立VPN网以对现有的网络作链路上的备份，而VPN的建立同时还可以实现移动办公，领导能在远程调用内网的资料进行远程决策服务和汇报工作。主要介绍VPN技术并叙述VPN技术在浙江省气象系统网络中的具体应用。     

MERLIN Images of Five Compact Symmetric Object Candidates at 1.6 GHz

1 INTRODUCTIONCompact Symmetric Objects (CSOs) are powerful and compact sources (overall size <1 bpc) with lobe emission on both sides of the central engine. The small size of these sourcesis almost certainly to be attributed to the youth of the sources themselves (ages < 104 yr) andnot due to a dense coallning medium (Readhead 1996). The unification scenario assumes thatCSOs evolve into compact steep spectrum (CSS) sotirces and then into Fanaroff-Riley type 11objects (Fanti 1995). …     

广西河池地区矿产资源勘查成果展销会情况介绍

１９９５年７月１１日—１２日，广西地矿厅和河池地区行署首次联合举办矿产资源勘查成果展销会，会址设在河池市，与会代表１３０余人。展销会提交大会展销的矿产资源勘查成果３８项，引资勘查项目３８项。经买卖双方三天的洽谈，涉及到的项目２５项，会议期间签订矿产资源勘查成果合同协议４项，愈千万元，取得较好的社会经济效益。为地勘资金多渠道来源开辟了新路子，对促进地勘单位地质找矿工作及勘查成果有偿使用发挥了重要作用     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Density perturbations in a Universe consisting of fluid plus collisionless neutrino gas

In a two-component Universe which consists of fluid (visible matter) plus collisinless massive neutrino gas (dark matter), the remarkable difference between the developed inhomogeneities in two components could be formed after the decoupling time. Whether the initial perturbation was in which of the two components, the inhomogeneities developed in visible matter are larger than that in neutrinos, especially on smaller scales. The necessary condition for such a situation to arise is only that the density of neutrinos in the Universe is dominant. That means the non-dominant visible component in the Universe is strongly clustered especially on smaller scales, while the distribution of the dominant dark matter (neutrinos) is fairly uniform.