长江口海域悬浮颗粒有机物的稳定氮同位素分布及其生物地球化学意义

对2006年2,5,8,11月份长江口海域表层水体中的悬浮颗粒物(SPM)进行稳定氮同位素分析,根据不同季节、不同区域内其1δ5N值的变化研究水体中氮的迁移、转化等生物地球化学过程,揭示其环境行为,从而对该海域的氮循环机制进行探索。研究发现:该海域悬浮颗粒有机物的稳定氮同位素组成(1δ5Np)分布范围较宽,在0.6‰~8.2‰之间,具有明显的时空分布特点,反映了不同程度的陆源输入和氮的生物地球化学过程的影响。口门内,表层水体中1δ5Np的变化主要受长江径流的陆源输入影响,生物地球化学作用影响较弱;最大浑浊带,水体中的悬浮颗粒有机氮受微生物的降解活动影响明显,各季节均存在不同程度的颗粒物分解作用;外海区,陆源输入减弱,悬浮颗粒物的δ15Np值主要受微藻的同化吸收作用以及一定程度的颗粒物分解作用影响。     

秦皇岛入海河流的污染水平、排放特征及对褐潮暴发区生源要素的影响

为查明秦皇岛近海大面积褐潮连年暴发的成因,在2013年3—11月对该地区主要入海河流和沿岸褐潮暴发区的生源要素污染进行了连续调查研究。调查结果表明,所调查入海河流普遍为劣V类地表水,总氮(TN)超标严重,按照氮污染程度由高到低排列依次为大蒲河洋河戴河石河汤河东沙河。各河流中的碳、氮、磷、硅等污染物浓度在时间变化上没有统一规律。基于综合污染指数法的评价结果显示,大蒲河和洋河为重度污染,戴河和汤河从先前的轻度污染加重为中度污染。从污染物入海量上看,TN和化学需氧量(COD)是排放量最高的两种河源污染物,其中溶解态氮占TN的74.6%。在所调查河流中,洋河和大蒲河分别贡献了TN的38.2%和33.2%,同时大蒲河还贡献了75.8%的活性磷酸盐和37.7%的活性硅酸盐,而75.9%的COD来自汤河、洋河和大蒲河。秦皇岛河源污染物排放在时间上较为集中在6—9月的丰水期,但各河流单独的排放具有随机性,没有统一的季节性规律,表现出受人为调控影响明显的特点。秦皇岛沿岸褐潮暴发区的生源要素变化与河源污染物排放有显著性相关(P=0.05)。     

改性黏土法消除球形棕囊藻赤潮的现场实验与效果评估

球形棕囊藻是一种具有复杂异型生活史的有毒有害赤潮生物,近年来在我国近海频繁暴发成灾,形成的巨大囊体为国内外罕见。迄今还未见到有效消除囊体型球形棕囊藻赤潮的方法报道。本文通过海上围隔实验和现场赤潮消除工程跟踪监测,考察了喷洒改性黏土消除囊体型球形棕囊藻赤潮的可行性与效率,并分析了改性黏土法治理赤潮时对水体及沉积环境的可能影响。围隔实验结果表明,喷洒改性黏土可以有效消除水体中过量的微藻细胞,其中以少量多次喷洒方法的效果最好,生物量(chl a)去除率90%。在对2016年2月广西防城港附近海域球形棕囊藻赤潮消除时的跟踪监测结果表明,改性黏土法适用于工程化消除赤潮作业,能够快速消除水体中的大量球形棕囊藻囊体,随改性黏土絮凝体沉入海底的赤潮藻可以快速分解而失去活性。喷洒改性黏土对于赤潮水体的主要理化指标影响不显著,所监测的水体COD、pH和不同形态氮、磷、硅等生源要素浓度都在原有水质水平范围内波动。     

改性黏土对褐潮生物种Aureococcus anophagefferens的去除研究

褐潮是一种由微微型藻引发的藻华,已连续4年在渤海海域暴发,给当地带来巨大的经济损失。目前,在褐潮尚不能有效预防、预控情况下,如何控制和减少其对生态环境和沿海经济带来的危害,成为一个急需解决的问题。利用改性黏土消除藻华是一种快速有效、并得以多次现场成功应用的方法。考察比较了多种不同产地、不同类型的黏土及其改性后对褐潮生物种Aureococcus anophagef ferens(抑食金球藻)的去除效果,选取5种典型的黏土作为主要的实验材料,开展了不同条件下黏土及其改性后对A. anophagef ferens去除效率的影响研究。实验发现,因A. anophagef ferens个体微小、藻华时密度大,相同用量的黏土或改性黏土对其去除效率低于常见的硅藻、甲藻生物;不同类型黏土对其去除效率有影响,高岭土的去除效率高于蒙脱土;而黏土改性后对A. anophagef ferens的去除效率提高3倍以上。在此基础上,还考察分析了黏土粒径、分散介质等因素对A. anophagef ferens去除效率的影响,为进一步研发安全、高效的褐潮应急消除材料做了有益探索。     

海水中溶解态铵盐氮同位素分析的预处理方法

以蒸馏法为基础, 研究了适合海水中铵盐稳定氮同位素分析的预处理方法。对蒸馏法预处理过程中的最佳蒸馏时间、沸石的选取、铵盐最适浓度范围等分别进行改进和优化, 获得稳定、高效的海水中铵盐氮稳定同位素分析预处理方法。结果表明: 最佳蒸馏时间为50 min, 铵盐的回收率为(97±5.8)%。测定了不同沸石对不同浓度氨氮的吸附率, 筛选出酸性条件下铵氮吸附效率较高且稳定的康华科技沸石; 确定铵盐的最佳适用浓度范围为2～10 μmol/L, 在该浓度范围内氮回收率94%～99%,氮分馏系数为0.1‰～0.8‰。将此方法应用于长江口海域水样分析, 结果表明, 这一方法可以应用于河口中铵盐的氮同位素分析, 能够为河口中溶解态氮的来源及循环机理等研究提供有效信息。     

Mechanisms and factors affecting the adsorption of sodium alginate onto modified clays

Algal organic materials (AOMs) are one critical factor affecting the efficiency of modified clays used for the mitigation of harmful algal blooms (HABs). This study was conducted to develop a deeper understanding of the mechanisms and factors affecting the adsorption of AOMs onto modified clays. Sodium alginate (polysaccharide) and kaolinite modified with polyaluminium chloride (PACl) were used as AOMs and modified clay model substances, respectively, and the effects of modifier dosage, contact time, solution pH and ionic strength were investigated through batch adsorption experiments. Kinetics revealed that the alginate adsorption rate was described well by a pseudo-second order model. PACl effectively enhanced the adsorption capacity of kaolinite and increased the adsorption rate, and the optimum additive amount of PACl was 5%. The experimental data fitted both the Freundlich and Langmuir adsorption equations well. The adsorption thermodynamics for alginate onto modified clays suggests that alginate adsorption is a spontaneous process. The adsorption of alginate onto modified clays was highly dependent on pH, with a decrease in adsorption observed with increased pH to 9.48, but the opposite was true above pH 9.48. Finally, adsorption increased with increasing ionic strength.     

不同黏土对中肋骨条藻的絮凝去除研究

比较了不同种类黏土对中肋骨条藻的絮凝去除效率, 分析了黏土理化特征对絮凝去除效率的影响。结果表明, 不同种类黏土的Zeta 电位为？36.1～？15.9 mV, BET 比表面积为0.31～60.76 m2/g, 硅铝比为1.35～3.62。当黏土用量均为0.50 g/L 时, 不同黏土对中肋骨条藻的絮凝去除速度和去除...     

基于水动力ROMS与BOX耦合模型的长江口及邻近水域水通量及水体交换特性

本研究将水动力模型ROMS(regional oceanic modeling system,区域海洋模式系统)与箱式(box)模型结合,详细阐述了长江口及邻近水域四个季节的水通量特征及水体交换特性。研究发现:总的水通量整体受季风控制,季风的作用在于使水体在南北方向上交替输送,而台湾暖流对春夏季底层水南向输运具有重要作用;直接进入123.5°E以东外海区域的水通量很小,而是先从南边界流出研究区域,然后通过海洋环流系统进入外海。在强烈季风下,水体更新依赖于季风方向的水平通量,主要是同层水体而不是表底层水体之间的交换。虽然水体更新时间较长的区域与缺氧区基本一致,但本研究认为该区域底层水体缺氧的本质原因是跃层阻隔了表底层水体之间的交换。     

Effects of modified clay flocculation on major nutrients and diatom aggregation during Skeletonema costatum blooms in the laboratory

Harmful algal blooms(HABs) can elicit several negative effects on aquatic environment(such as depleting the oxygen, blocking the sunlight, destroying the habitats of organisms) and life health(including poisoning/killing marine mammals, birds and human). Among the various control strategies for HABs(physical manipulation needs lots of manpower and expensive equipment, chemicals treatment has some toxic byproduct and high residual, microbial agents only has limited in laboratory research), the coagulation-flocculation of HAB species by modified clay(MC) has been proven to be an effective, lowcost and environmentally friendly method that has been widely applied in the field, particularly in eastern Asia. In order to examine the long-term effects of MC treatment, this study investigated the alternations in seawater of Skeletonema costatum, a high biomass dominant HAB species along the Chinese coast, by comparing the degradation of S. costatum detritus(A1) with the application of MC treatment(A2) and MC treatment in sediment condition(A3). The low dosage of 0.25 g/L MC could efficiently remove 4×108 cells/L of S. costatum cells within 3.5 h(approximately 97% removal). In addition, the results showed that both inorganic and organic nutrients were effectively reduced from seawater by MC particles. Compared to the total nitrogen(TN) and total phosphorus(TP) concentrations in A1 seawater, 44% of TN and 93% of TP in A2 seawater, as well as 72% of TN and 93% of TP in A3 seawater were removed during the onemonth incubation period. Simultaneously, 64% of DISi in A2 and 44% of DISi in A3 significantly decreased( P 0.001). This study demonstrated that MC treatment was able to significantly increase the downward flux of nutrients and delay the release velocity of inorganic nutrient from MC-algae matrix into the overlying seawater, particularly within sediment environment.     

Environmental characteristics of annual pico/nanophytoplankton blooms along the Qinhuangdao Coast

Blooms of some pico/nanophytoplankton have occurred frequently along the Qinhuangdao coast since 2009, and it is necessary to identify the critical environmental factors inducing them. In this study, variations in the physical and nutrient characteristics of the seawater were analyzed following the development of local blooms in 2013. The local environmental characteristics were also compared with those of the Changjiang River estuary, China, and the Long Island estuaries in the USA, which are also prone to blooms of special algal species. In Qinhuangdao the local water temperature varied seasonally and rose above 15°C in 2013 early summer, coincident with the water discoloration. The salinity was more than 28 with a variation range of 3 throughout the year. Our results suggest that the physical conditions of the Qinhuangdao coastal area were suitable for the explosive proliferation of certain pico/nanophytoplankton, e.g. Aureococcus anophageff erens. The water supporting the bloom was not in a condition of serious eutrophication, but there were relatively high concentrations of reduced nitrogen(especially ammonium), which acted as an important nitrogen source for the pico/nanophytoplankton bloom. There was also a large gap between total nitrogen(TN) and dissolved inorganic nitrogen(DIN). Although the phosphate concentration was relatively low, there was no evidence of phosphorus limitation to the growth of pico/nanophytoplankton during bloom events.