Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.     

修武局扮亮国土资源管理的"窗口"

国土资源管理体制改革以来,修武县国土资源局把基层所规范化建设作为提升国土管理水平的重要工作来抓,扎扎实实开展了一系列标准化国土所创建活动,城关、方庄、郇封三个国土资源所先后被县委、县政府评为"先进国土所".     

修武县狠抓集体土地所有权登记发证工作

本刊讯 2003年,修武县将集体土地所有权发证作为工作重心,通过培训骨干,制定方案,开展试点等措施有效地推动了工作的顺利进行。 目前,该县10个乡(镇)共签订权属协议书580余份,调解纠纷6起,128个行政村已确权发证,确权面积380多平方公里,发证率达60%。■修武县狠抓集体土地所有权登记发证工作@姜振茎     

修武县国土资源局信访工作实现开门红

2006年元月4日，一桩长达半年之久．跨越两个年度的信访案件，在修武县国土资源局信协工作人员的多方努力下得以圆满解决，为该局在新的一年里做好信访工作开了个好头。     

修武局扮亮国土资源管理的"窗口"

国土资源管理体制改革以来,修武县国土资源局把基层所规范化建设作为提升国土管理水平的重要工作来抓,扎扎实实开展了一系列标准化国土所创建活动,城关、方庄、郇封三个国土资源所先后被县委、县政府评为“先进国土所”。抓投入二百万元打造标准化站所在体制改革初期,修武县几乎没有一个国土资源所拥有自己的办公房屋,大部分是临时借用当地乡镇政府的办公用房或租用其它单位的办公室,办公条件比较差。在“完善体制,提高素质”活动中,修武局加大硬件投入,改善办公条件,推进了基层国土所规范化建设。今年以来,该局投入二百多万元全面建设基层…     

焦作市强化环境水文地质监测工作

为准确掌握地下水的水位、水质、水量及水温的动态变化及地下水运移规律,监测、监督、防止地下水的过量开采与污染,自2005年以来,焦作市国土资源局在区内开展了环境水文地质监测工作。作为一项新的职能,焦作市国土资源部门克服无专业机构、无专业技术人员、无监测设备等困难,从零做起,腾出专门办公场所,配置办公设备和监测设备,抽调专业技术人员,从水位、水质监测入手,逐步全面开展了各项工作。目前,该局已在市城区范围内布设长观点11个,其中国家级5个、一般级6个;建立水质监测点2个,布设统测点41个,监测控制面积达415平方公里,形成了比较完…     

织锦巴菲蛤(Paphia textile)过氧化氢酶基因cDNA全长克隆、序列同源分析与组织表达

Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile(Pt CAT) was cloned using RTPCR and rapid amplification of c DNA ends(RACE). Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif(~(61)FNRERIPERVVHAKGAG~(77)), a proximal heme-ligand signature sequence(~(352)RLFSYSDP~(359)), and three catalytic amino acid residues(H~(72), N~(145), and Y~(356)). Pt CAT also contains two putative N-glycosylation sites(~(34)NKT~(36) and ~(437)NFT~(439)) and a peroxisome-targeting signal(~(511)AQL~(513)). Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA: first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.     

绵阳市近36年来气候变化趋势浅析

一、引言 绵阳市位于四川盆地西北部、嘉陵江支流涪江的上游,地势由西北向东南倾斜。绵阳市近36年来的气候变化,相对全球而言,仅是小范围的局部气候变化现象;从变化的时间尺度来讲,30几年显然属于气候短期变化。本文旨在分析绵阳市近30多年来温度、降水     

操作CTM-DI磁力仪时应该注意的事项

本文介绍了CTM-DI磁力仪使用时应该注意的事项及维护方法。