云南永胜宝坪浅源热液改造型铜矿地质及成因

宝坪铜矿赋存于三叠系下统腊美组中。矿体呈似层状,少量脉状,严格受北北西-北西向构造控制。矿床成因类型属浅源热液改造型铜矿床。     

高速公路沿线暗浜填土的勘察和评价

目前，线路勘探中对浜填土检测不够重视或缺乏有效的方法，使浜填土的处理缺乏针对性，造成不必要的浪费或工程隐患。该文探讨了在线路勘探中暗浜的勘查技术及相应评价与整治方法。     

响水县在“五个环节”上下功夫

2003年,江苏省响水县民政局为适应新形势,力创新特色,把地名标志设置引入市场机制,努力探索地名设置工作可持续发展路子。该局利用市场化运作方式,仅用一个月时间,完成了县城街、路牌规范设置,实现了地名标志设置标准化。不仅为美化县城增添新的亮点,而且也方便了广大客商和人民群众,受到广泛关注和好评。     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.     

西太平洋深海海水中乙醛降解菌多样性及群落结构研究

As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.     

由sPn波确定观测台地区的地壳厚度

本文在多年资料分析工作的基础上,总结、确认了sPn波的特征和时速性质,并利用sPn波随震中距变化甚小而随震源深度变化较大的特点,确定了观测台地区的地壳厚度。为浅源地震确定地壳厚度提供了一种方法。     

长江口大型桩基码头勘探中的单元体划分问题

由于桩基持力层的岩性岩相变化十分复杂，应用常规的工程地质单元体划分方法时常出现分现混乱、使用困难等问题，现以第四纪地质研究为基础、以桩基工程评价需要为目的，改进了工作方法，获得较好的工程效果。     

计算机直接制版技术

本文通过对计算机直接制版（CTP）技术的主要目标阐述和实现目标相关的软硬件的发展论证，展示了印前技术的发展趋势。     

东昆仑活动断裂带的强震构造条件及未来强震危险区分析

通过对东昆仑活动断裂带强震构造条件的研究发现，这条断裂带的强震构造条件不仅与所处的区域地壳运动状况有关，而且与该活动断裂带的规模、形态，断裂带的构造组合及断裂带的运动条件等密切相关。在研究了东昆仑活动断裂带强震构造条件的基础上，还沿断裂带划出了三个未来强震危险区。