CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

贵州普定陈旗堡地区土体主要理化性质与持水量相关性研究

根据贵州普定陈旗堡典型喀斯特地区的生态特性,从水文地质与工程地质角度,借助土壤学对土壤水分特性的研究理论,对表层土壤20cm以下土层的土体物理化学性质与土体水分含量,即土体的持水量（waterholding capacity),进行了相关性研究。分析结果表明：土体颗粒成分、孔隙比、矿物成分含量等对土体水分含量均有影响。土体中粉粒含量较高而粘粒含量较少,且粒度分配不均匀时,土体水分含量相对较高;孔隙率高的土体水分含量较高;伊蒙混层矿物、伊利石和绿泥石等粘土矿物含量较高时,土体水分含量相对较高,而长石、碳酸盐等原生矿物含量较高时,土体水分含量相对较小。石英对于土体持水量影响不明显。     

福建省东部浅层地下水地球化学

本文对福建东部地区3.8万平方公里陆地浅层地下水(井水)1206件样品中19种元素的含量特征、元素间的相关关系、元素空间分布特点与地质背景的关系等方面进行了研究。研究表明,该地区浅层地下水中以Ba、Cl-、F-、Fe、Mn、Sr、Zn元素含量高、变化幅度大为显著特征;元素含量与pH没有明显的相关关系,Cl-、Sr、Co、Ni、Pb等元素间则具显著的正相关关系,Cl-具有决定性作用;地下水中元素的空间分布特征受地质背景及地理条件的制约。     

钱二块铀矿床采铀注液结垢趋势的理论分析与预测

用离子系数矩阵法对钱二块铀矿床地浸采铀试验注液中存在的独立离子反应进行了确定,由溶度积规则及反应平衡原理,借助Matlab编程,估算出了钱二块铀矿床地浸采铀试验注液可能会生成的沉淀物种类及数量,为防治结垢提供了一定的理论依据.     

GAS参数优化方法在黄河上游非汛期径流预报中的应用

本文采用相关分析与GAS参数优化相结合的方法,对黄河上游非汛期径流预报模型进行了研究,并对2000～2003年的径流进行了实时预报检验,从检验结果来看,这种预报方法具有较高的预报精度.本文为非汛期径流预报研究提供了参考和借鉴,同时,就计算分析手段而言,GAS在参数优化方面具有广泛的应用价值.     

川东华蓥山二叠系／三叠系界线附近粘土层中粘土矿物的类型及成因

对川东华蓥山二叠系／三叠系界线附近粘土层中的粘土矿物进行野外采样和室内X射线衍射分析,结果表明,这些粘土矿物主要由规则混层伊利石-蒙脱石组成,其中1个样品可能舍有少量不规则混层绿泥石-蒙脱石等粘土矿物。总地看来．该界线粘土层中的规则混层伊利石-蒙脱石中伊利石层的含量自下而上呈现减少的趋势,平均含量约83％,所经历的最大古地温在125-150℃之间。这些粘土矿物的构成和特征与前人在华南其他地区相同层位取得的成果总体上具有较好的一致性．显示了该粘土层成因的相同性及所具有的等时意义。不同地区粘土矿物构成的差异性（如高岭石的存在与含量）可能与二叠系／三叠系界线不整合的存在与否或间断时间有关,规则混层伊利石-蒙脱石的混层比则与埋藏历史和沉积-沉积期后的流体介质有关。     

广西合浦海水珍珠变黄机理新议

海水珍珠缓慢变黄的传统解释是无机相文石不稳定向方解石转化这个不可逆过程造成的.然而作者通过肉眼观察、光学显微镜观察、H2O2漂白体系使变黄的海水珍珠重新变白实验及X射线粉晶衍射分析,均表明传统解释可能存在错误,变黄的海水珍珠无机相没有发生明显的相变,故推测珍珠内有机相的变化是珍珠变黄的主要原因.     

青岛液体化工码头苯罐泄漏对大气环境的影响

预测和评价了青岛港液体化工码头苯贮罐苯泄漏对大气环境的影响．结果表明，苯罐一旦发生泄苯事故，会对大气环境造成不同程度的影响；在最不利天气条件下，泄漏挥发量为3375kg，会导致22人死亡；泄漏挥发量为1237kg，会导致4人死亡；如果同样的泄苯挥发量，发生在微风条件下，则分别有4人死亡和无人死亡；若发生33kg泄漏挥发量，无论在何种天气条件下，都不会导致人员伤亡。     

不同浓度的营养盐(NO3-N,PO4-P)条件下Pb(Ⅱ)对2种海洋赤潮藻生长影响的研究

探讨海区环境容量的估算,为海水水质标准的制订提供理论依据,采用1次培养实验方法,研究了3种不同浓度的营养盐(NO3-N,PO4-P)条件下Pb(Ⅱ)对旋链角毛藻(Chaetoceroscurvisetus)、中肋骨条藻(Skeletonemacostatum(Greville)Cleve)的生长影响.采用抑制率-重金属离子浓度的对数方程拟合求算出3种浓度营养盐(NO3-N、PO4-P)条件下Pb(Ⅱ)对2种赤潮藻的96hEC50值,分别为8373,2563,2307;18523,10445,3494μg·dm-3,并根据方程应用origin7.0拟合3种不同浓度的营养盐(NO3-N,PO4-P)条件下旋链角毛藻和中肋骨条藻在不同浓度Pb(Ⅱ)条件下的生长曲线.实验结果表明,较低浓度Pb(Ⅱ)对旋链角毛藻和中肋骨条藻生长都有一定的促进作用,最佳促进藻类生长浓度分别为454,258,51;351,194,35μg·dm-3;高浓度Pb(Ⅱ)对它们的生长则表现为抑制作用,并且随着营养盐(NO3-N,PO4-P)浓度的增加,Pb(Ⅱ)对两种赤潮藻生长的抑制作用是逐渐降低的,营养盐(NO3-N,PO4-P)与Pb(Ⅱ)之间可能存在着拮抗作用.     

Numerical Study on Instantaneous Discharge of Unsorted Particle Cloud in Cross Flow

The mixing characteristics of particles such as dredged sediment of variable size discharged into cross flow are studied by a 3D numerical model, which is developed to model the particle-fluid two-phase flow. The Eulerian method with the modified k-ε parameterization of turbulence for the fluid phase is used to solve fluid phase, while a Lagrangian method for the solid phase (particles), both the processes are coupled through the momentum sources. In the model the wake turbulence induced by particles has been included as additional source term in the k-ε model; and the variable drift velocities of the particles are treated efficiently by the Lagrangian method in which the particles are tracked explicitly and the diffusion process is approximated by a random walk model. The hydrodynamic behavior of dumping a cloud of particles is governed by the total buoyancy of the cloud, the drag force on each particle and the velocity of cross-flow. The computed results show a roughly linear relationship between the displacement of the frontal position and the longitudinal width of the particle cloud. The particle size in the cloud and the velocity of cross flow dominate the flow behavior. The computed results are compared with the results of laboratory experiments and satisfactory agreement is obtained.