CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Study of charge structure and radiation characteristic of intracloud discharge in thunderstorms of Qinghai-Tibet Plateau

The comprehensive observations on lightning discharges were conducted in Naqu area of Qinghai-Tibet Plateau in summer of 2002. The electric structures of thunderstorms and the characteristics of lightning discharges at initial stage were analyzed by using the observation data. The results show that most of intracloud (IC) lightning flashes were polarities inverted in thunderstorms with tripole electric charge structure and occurred between negative charge region located in the middle of the thunderstorm and positive charge region located at the bottom of the thunderstorm. The radiation characteristics of discharge processes in cloud with longer lasting time involved in Cloud-to-Ground (CG) lightning flashes were similar to that of IC discharges.A lot of radiation pulses were produced in these discharge processes. Because the IC discharges took place at the bottom of thundercloud and were near the ground, they may produce more serious damage to equipment on the ground therefore should not be neglected in lightning protection.     

沙丘不同部位土壤呼吸对人工降水的响应

 利用LI-8100土壤呼吸测量仪,对古尔班通古特沙漠南缘阜康北部地区沙丘不同部位（坡底、坡中、坡顶）的土壤呼吸速率进行了测量,探讨了沙丘不同部位土壤呼吸速率对降雨的响应,分析了土壤水分和土壤温度对土壤呼吸速率的影响。结果表明:①沙丘不同部位土壤呼吸速率的日变化呈“双峰曲线”,而增雨处理后,土壤呼吸速率的日变化曲线大部分转变为“单峰曲线”。②增雨处理增加了沙丘不同部位土壤呼吸速率的变化幅度、平均值和极差,推后了土壤呼吸速率最大值到来的时间。③土壤呼吸速率与土壤温度的相关性对降雨表现出积极的响应,降雨改变了土壤温度的日变化曲线类型,提高了土壤温度与土壤呼吸速率的相关系数。④非增雨处理时,沙丘坡底、坡中和坡顶的土壤呼吸速率与土壤水分的相关性系数均较高,而增雨处理后,土壤呼吸速率与土壤水分的相关系数有所下降,仅坡中的相关系数通过了α=0.01的显著性检验。     

Iron Isotopic Fractionation and Origin of Chromitites in the Paleo-Moho Transition Zone of the Kop Ophiolite, NE Turkey

The Kop ophiolite in NE Turkey is a fragment of Neo-Tethyan forearc.It can be mainly divided into a paleo-Moho transition zone(MTZ)in the North and a harzburgitic mantle sequence in the South.Dunites are predominant in the MTZ of the Kop ophiolite,and they are locally interlayered with chromitites and enclose minor bodies of harzburgites near the petrological Moho boundary.Large Fe isotopic variations were observed for magnesiochromite(-0.14‰to 0.06‰)and olivine(-0.12‰to 0.14‰)from the MTZ chromitites,dunites and harzburgites.In individual dunite samples,magnesiochromite usually has lighter Fe isotopic compositions than olivine,which was probably caused by subsolidus Mg-Fe exchange between the two mineral phases.Both magnesiochromite and olivine display an increasing trend ofδ56Fe along a profile from chromitite todunite.This trend reflects continuous fractional crystallization in a magma chamber,which resulted in heavier Fe isotopes concentrated in the evolved magmas.In each cumulative cycle of chromitite and dunite,dunite was formed from relatively evolved melts after massive precipitation of magnesiochromite.Mixing of more primitive and evolved melts in the magma chamber was a potential mechanism for triggering the crystallization of magnesiochromite,generating chromitite layers in the cumulate pile.Before mixing happened,the primitive melts had reacted with mantle harzburgites during their ascendance;whereas the evolved melts may lie on the olivine-chromite cotectic near the liquidus field of pyroxene.Variable degrees of magma mixing and differentiation are expected to generate melts with differentδ56Fe values,accounting for the Fe isotopic variations of the Kop MTZ.     

富水破碎岩体注浆加固材料试验研究与应用

针对富水破碎地层注浆治理过程中传统水泥类材料难以实现注浆加固和堵水同步进行的难题,以硫铝酸盐水泥熟料和钢渣微粉为主要原材,成功制备了一种水泥基复合注浆材料（CGM）。通过与传统水泥材料进行性能对比试验,采用扫描电镜和X射线测试手段,分析了CGM材料制备工艺、组分含量和浆液制备条件对材料性能的作用规律,并检验了CGM材料的工程适用性。结果表明：CGM材料宜采用混合粉磨制备工艺,可显著提高其水化活性,且粉磨时间应不超过45 min。钢渣微粉含量越高,水灰比越大,结石体强度越低,凝结时间越长,水灰比超过1.2:1时,结石体后期会出现干缩。与传统水泥材料相比,CGM材料浆液凝结时间与黏度的环境敏感度低,具有显著的工程适用性和性能优越性。     

持久性有机污染物对环境的影响及对策

概述了《斯德哥尔摩公约》中规定的12种持久性有机污染物及六六六、多环芳烃等持久性有机污染物,以及持久性有机污染物对土壤、水体、农产品及大气的污染状况,并在借鉴国内外先进经验的基础上,根据中国的国情提出了5项对策.     

物源分析的研究与展望

物源分析在盆地分析中具有重要意义,是沉积盆地的大地构造背景判别、古环境恢复的重要依据。本文简要回顾了砂岩成分,阴极发光、古水流流分析以及主元素和重矿物组合分析在物源研究中的应用,着重阐述了稳定同位素测年技术、风化强度的量化ＲＥＥ和痕量元素等地球化学方面的新技术和新方法在物源分析中的应用及其前景,在此基础上指出物源分析应强调利用多种方法进行综合分析,才能获得准确的沉积物源信息。     

牙鲆肠淋巴样组织免疫细胞研究Ⅱ.无颗粒白细胞的超微结构

运用电镜技术,在牙鲆肠淋巴样组织鉴定出４种无颗粒白细胞,即淋巴细胞、巨噬细胞、单核细胞和浆细胞样细胞。它们的形态均比在造血器官中多样化。巨噬细胞形态极其有规则;单核细胞的细胞质中有较多空泡;淋巴细胞具多且细长的伪足,而浆细胞样细胞是以内含大量的粗面内质网为其主要特征。同时,作者对无颗粒白细胞的形态与功能之间的关系进行了探讨。     

Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187

Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively tested effects of A23187, the calcium ionophore, on the development of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal blastomeres committed to endoderm were treated with A23187 prior to gastrulation, they were transformed into ectodermal cells as evidenced by the cell morphology and function characteristic of epidermis. However, the developmental fate of the same blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged. Thus, vegetal-endodermal cells in amphioxus embryos are not irreversibly determined before the gastrula stage, and artificial increase in intracelluar Ca²⁺ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells. Project 39470091 supported by NSFC and partly supported by Shandong Natural Science Foundation, Grant No.93D0140.     

Laboratory observation on spawning,fecundity and larval development of amphioxus (Branchiostoma belcheri Tsingtaunese)

Although amphioxus is widespread in temperate and tropical seas, its population is diminishing because of environmental pollution. To keep the population of this evolutionarily important animal from diminishing, study on its reproduction and development is necessary. The main findings in this study on the spawning and fecundity of the amphioxus reared in laboratory and its larval development are as follows. 1. Water temperature markedly affected the spawning. It spawned only when water temperature reached 21°C. 2. Spawning of the amphioxus in laboratory was markedly extended. Initially, the amphioxus spawned at about 7:00 PM, but spawning time was postponed as spawning days went on. 3. The number of eggs produced by a female ranged from 1400 to 12800, average of 5800. This also represents the fecundity of the amphioxus because it shedded all eggs within the ovary at a time. 4. During the first few months of life of the amphioxus, its growth rate changed seasonally. The growth rate in summer and fall was greater than that in winter. 5. The pelagic larva became a benthic adult after 50 days. 6. The amphioxus reared in laboratory from fertilized eggs could produce fertile eggs and sperms. These findings can be a foundation for measures to address the problem of diminishing amphioxus population. Contribution No. 2274 from the Institute of Oceanology, Chinese Academy of Sciences This work was supported by CNNSF, and Chinese Academy of Sciences.