Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

Pulsar magnetic field for an infinitely conductive accretion disk

The magnetic-field distribution outside a flat, infinitely conductive unbounded disk in the field of a point magnetic dipole is determined. A relationship is established between the problem of magnetic-field determination and the problem of the flow of an ideal incompressible fluid around an infinitely thin disk.     

Stochastic modelling of groundwater flow and solute transport in aquifers

This paper presents an introductory overview of recently developed stochastic theories for tackling spatial variability problems in predicting groundwater flow and solute transport. Advantages and limitations of the theories are discussed. Lastly, strategies based on the stochastic approaches to predict solute transport in aquifers are recommended.     

Pulsar Wind Nebulae in Egret Error Boxes

A remarkable number of pulsar wind nebulae (PWN) are coincident with EGRET γ-ray sources. X-ray and radio imaging studies of unidentified EGRET sources have resulted in the discovery of at least six new pulsar wind nebulae (PWN). Stationary PWN (SPWN) appear to be associated with steady EGRET sources with hard spectra, typical for γ-ray pulsars. Their toroidal morphologies can help determine the geometry of the pulsar which is useful for constraining models of pulsed γ-ray emission. Rapidly moving PWN (RPWN) with more cometary morphologies seem to be associated with variable EGRET sources in regions where the ambient medium is dense compared to what is typical for the ISM.     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

Coronal change at the south-west limb observed by Yohkoh on 9 November 1991, and the subsequent interplanetary shock at Pioneer Venus Orbiter

A spectacular change in the lower corona on the south-west limb has been found in solar images taken by the Yohkoh soft X-ray telescope. The event is characterized by a large topological change in magnetic field and a large intensity decrease observed after the X1. 1/1B flare on 9 November, 1991. A coronal mass ejection (CME) was observed by the Mark III K-coronameter (MK3) at the HAO/Mauna Loa Observatory. Both the MK3 (white-light) and soft X-ray observations showed that one leg of this CME was located above the flare site. An interplanetary shock associated with this event was observed by Pioneer Venus Orbiter, and, possibly, by IMP-8.Also Cooperative Institute for Research in the Environmental Sciences (CIRES), University of Colorado, Boulder, CO 80309, U.S.A.