病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

Rain-Flow and Reverse Rain-Flow Counting Method for the Compilation of Fatigue Load Spectrum

The rain-flow counting method is widely used to compile the fatigue load spectrum. The second stage counting of the rain-flow method is a troublesome process. In order to overcome this drawback, the rain-flow and reverse rain-flow counting method is proposed in this paper. In this counting method, the rule for counting of the rain-flow method is modified, so that the sequence of load-time need not be adjusted. This is a valid and useful method to count cycles and compile the load spectrum and it can be widely used in ocean engineering.     

培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响

本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2，并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析，旨在了解不同培养基对菌株生长和活性物质合成的影响．结果显示，PT2菌株在不同培养基上的培养特征及生长速率有所不同，液体培养基发酵液抽提物的抗肿瘤活性也有较大差别，其中SYE发酵液抽提物活性最强，对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7．6μg／cm^3、2．4μg／cm^3和3．3μg／cm^3．采用HPLC法对不同抽提物进行分析，发现它们之间的组成成分有显著差异．本文的实验结果表明，不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。     

黑鲷(Acanthopagrus schlegeli)肿瘤坏死因子α cDNA的克隆及特征分析

采用同源克隆的方法，从黑鲷中获得肿瘤坏死因子(TNFα)全长cDNA序列。该序列含1288个核苷酸，可编码253个氨基酸的TNF前体蛋白。它是一种跨膜蛋白，无糖基化位点和信号肽结构。黑鲷TNFα与其它鱼类的TNFα的相似性很高，占据进化上独立的分支；与哺乳类TNFα和TNFG源于共同的祖先。基因结构分析显示，该基因含有TNF家族pmfile和TNF家族的标签序列；在参与TNFα基因二硫键形成的两个半胱氨酸和TNFα三聚体形成的位点高度保守；空间结构模拟显示，它与哺乳类TNFα的空间结构相似，都是由两个β折叠片组成，每个折叠片包含5个反向平行的β链。表达研究结果表明，黑鲷TNFα在检测的各个组织中均为组成型表达，表现为在刺激与非刺激鱼体中，都可以检测到黑鲷TNFα的表达，但是其表达水平在不同组织中有很大差异。黑鲷TNFα在头肾、脾脏和鳃的表达量较高，而在心脏、肝脏、血液、肾脏和大脑中表达量较低。     

Tgd对双频观测值电离层模型的影响分析

采用双频观测值建立穿刺点上的VTEC模型,说明了Tgd因素的影响,用实际数据分析了其影响。     

大冶铁矿国家矿山公园规划实践

大冶铁矿的开发利用在为我国的钢铁事业作出巨大贡献的同时,也给矿区的地表环境、森林资源带来了很大的破坏。矿山公园的规划实施可极大地改善矿山环境,为矿山的可持续发展提供较好的途径。     

Evolution from Oceanic Subduction to Continental Collision: a Case Study from the Northern Tibetan Plateau Based on Geochemical and Geochronological Data

Two apparently distinct, sub-parallel, paleo-subduction zonescan be recognized along the northern margin of the Tibetan Plateau:the North Qilian Suture Zone (oceanic-type) with ophioliticmélanges and high-pressure eclogites and blueschistsin the north, and the North Qaidam Belt (continental-type) inthe south, an ultrahigh-pressure (UHP) metamorphic terrane comprisingpelitic and granitic gneisses, eclogites and garnet peridotites.Eclogites from both belts have protoliths broadly similar tomid-ocean ridge basalts (MORB) or oceanic island basalts (OIB)in composition with overlapping metamorphic ages (480–440Ma, with weighted mean ages of 464 ± 6 Ma for North Qilianand 457 ± 7 Ma for North Qaidam), determined by zirconU–Pb sensitive high-resolution ion microprobe dating.Coesite-bearing zircon grains in pelitic gneisses from the NorthQaidam UHP Belt yield a peak metamorphic age of 423 ±6 Ma, 40 Myr younger than the age of eclogite formation, anda retrograde age of 403 ± 9 Ma. These data, combinedwith regional relationships, allow us to infer that these twoparallel belts may represent an evolutionary sequence from oceanicsubduction to continental collision, and continental underthrusting,to final exhumation. The Qilian–Qaidam Craton was probablya fragment of the Rodinia supercontinent with a passive marginand extended oceanic lithosphere in the north, which was subductedbeneath the North China Craton to depths >100 km at c. 423Ma and exhumed at c. 403 Ma (zircon rim ages in pelitic gneiss). KEY WORDS: HP and UHP rocks; subduction belts; zircon SHRIMP ages; Northern Tibetan Plateau     

基于ODBC的地震前兆数据批量卸出与转换程序的实现

介绍了CDBC技术、工作流程及数据传递方式；分析了研制数据卸出与转换程序的关键技术及实现方法；描述了程序的组织结构。通过实例说明了该程序在全国前兆台网中心卸载与转换地震前兆数字化的观测数据中的应用。     

遥测地震仪发射机电路分析及检修

在无线遥测地震仪器中 ,发射机长期使用容易出现输出功率下降、无输出功率、发射频率不准等故障。分析了发射机典型电路——石英晶体主振电路、倍频电路、激励级电路、功率放大级电路的基本原理。排除发射机的故障时 ,先从不同单元电路逐一检查、测试 ,判断故障类型 ,最后进行发射机总调试     

Microbial production of CO2 in red soil in Stone Forest National Park

Lunan stone forest is a kind of typical karst in China, which is mainly developed under red soil. In the winter of 1999, three study sites were chosen in stone forest national park according to vegetation cover, geomorphologic location and soil types. CO2 concentration was measured with Gastec pump at different depths of soil (20, 40, 60 cm) and at the same time soil samples were gathered and soil properties such as soil moisture, pH, soil organic content were analyzed and the total number of viable microbes were counted in laboratory. In the study, dependent variable was chosen as the mean soil log (PCO2), and soil properties were chosen as the independent variables. Multiple stepwise regression analysis showed that the total amount of microbes and soil moisture are the best indicators of the CO2 production, with the equation LOG(PCO2) = - 0.039(TNM) - 0.056(Mo) + 1.215 accounting for 86% of the variation of the soil CO2 concentration, where TNM is the total number of microbes in the soil and Mo is the moisture of soil sample.