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Crude fucoidanase was extracted from digestive organ of a herbivorous marine gastropod LuneZla cornata coreensis. The characteristics of fucoidanase dynamics e.g. optimum pH,optimum temperature, activators, inhibitors, Michaelis constant(Km) and maximum velocity (Vmax) were assayed. According to these characteristics, two kinds of fucoidanase defined as fucoidanase A and B might exist in the crude extract. Purification of fueoidanase was carried out by the method of ammonium sulfate-precipitation and the enzymic preparation of acetone powder was made. 相似文献
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在海洋腹足类动物的消化器官内,发现褐藻糖胶酶(fucoidanase)的甚少。Galli等在一种食植性马蹄螺(Tegula funebralis)的中肠提取液,以及Alexander等在红树林潮间带一种食碎腹足类(Telescopium telescopium)的晶杆中都测得褐藻糖胶酶的活性。Alexander等还报道了该酶的最适pH为6,饱和底物浓度为6mg/ml°有关该酶的其他反应动力学特性未见报道。 我们在研究三种海螺,食植性的朝鲜花冠小月螺(Lunella coronata coreensis)、杂 相似文献
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