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In October and November of 1977 and September and October of 1978,a quantitative survey of benthic animals was conducted in the East China Sea Continental Shelf and its adjacent waters(26°00'-34°00'N,124°00'-129°00'E,water depth 39-1550m),where 307 stations were dredged and 1200 samples collected. 相似文献
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本文主要介绍了1988年寿光县对虾养殖期间(5~9月)水环境和底泥环境中微生物种群的变化。据不同的地理位置,选择不同的养殖虾池;分别将水样和底泥环境中的弧菌、异养菌,及硫酸盐还原菌进行培养、分离计数。据对虾养殖期5个月的数据分析,不同的养殖虾池有不同的菌数波动范围。底泥与水环境中的菌数分布各有不同。异养菌在水样中的分布最高值为736×10~2个/ml,最低值为268.1×10~2个/ml;湿泥样中的最高值为372.7×10~4个/g最低值为125.9×10~4个/g。弧菌在水样中的最高值为12.33×10~2个/ml,最低值为0.5×10~4个/ml。据综合资料分析,对虾养殖池中的各种细菌分布,底泥高于水样,高温季节高于低温季节。底泥和水环境中有机物的含量与细菌数的分布成正比。底泥中硫酸盐还原菌的数量,是底泥环境中有机物污染程度的标志之一。根据硫酸盐还原菌的数量分布,可以有效地控制有机质(如对虾饵料)的投放量等。 相似文献
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Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome 总被引:1,自引:0,他引:1
An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified
and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing
four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309) 相似文献
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中国北方养殖牙鲆的淋巴囊肿病一般发生在水温较低的10~12月,之后随水温的升高肿瘤脱落、出现自愈现象.搞清自愈机理.是有效抑制和减少淋巴囊肿病的重要科学内容.本文研究了不同饲育水温下健康和患淋巴囊肿病牙鲆细胞因子的表达变化,仞步探讨了细胞因子在肿瘤自愈中的作用.通过模拟养殖中的水温变化,采用荧光定量PCR(re-al-time fluorescence quantitative PCR)技术.检测了14,18,22和26℃下牙鲆肝、脾、头肾组织中6种细胞因子(白细胞介素1β(1L-1β)、Mx蛋白(Mx)、肿瘤坏死因子(TNF)、肿瘤坏死因子受体(TNFR)、趋化因子(IL-8)和趋化因子受体(IL-8R))表达变化.结果表明.患病牙鲆肝、脾、头肾组织中的IL-8、IL-8R、Mx、IL-1β、TNF和TNFR-1表达量随温度升高而显著升高;健康牙鲆上述细胞因子的表达基本未出现随温度升高而显著上升的现象.本试验结果响应了养殖水温升高肿瘤脱落的现象,提示上述细胞因子在淋巴囊肿病自愈过程中可能发挥了抗病毒作用. 相似文献
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Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder. 相似文献
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Cladistic analysis was used to clarify the phylogeny of 16 genera of yeasts whose great morphological differences and inclusion in different classification systems resulted in controversies over the taxonomy of seven genera such as Crypeococcus, etc. Some scholars suggest that they belong to Ascomycetes. but others think they belong to fungi imperfecti. After comprehensive cladistic analysis of many genetic characters, the authors consider that the above-mentioned seven genera of yeasts developed in parallel with Ascomycetes so that they should belong to one and the same developmental system. 相似文献
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中国台湾地区及世界部分主要海水养殖国家养殖疾病综述 总被引:2,自引:0,他引:2
本文综述了中国台湾地区及世界部分主要海水养殖国家和地区的水产养殖病害情况,对鱼虾贝类的病毒性疾病,细菌怀疾病及其他病症的流行情况,防治方法,病原特征,宿主范围和组织病理学特征等进行了较详细的介绍。本综述对我国的海水养殖业和水产病害研究将有重要的指导作用。 相似文献
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氨氮胁迫对刺参几种免疫酶活性的影响 总被引:3,自引:0,他引:3
以养殖刺参(Apostichopus japonicus)为研究对象,对不同浓度的氨氮处理及病菌感染条件下刺参体腔液免疫酶的变化进行了测定.结果表明:随着氨氮处理强度的增加,刺参体腔液中超氧化物歧化酶(SOD)、碱性磷酸酶(ALP)、谷胱甘肽过氧化物酶(GPx)及溶菌酶(LSZ)活性呈现先升高后降低的趋势,而同时感染病菌的情况下,较低质量浓度的氨氮胁迫可使SOD、GPx、ALP及LSZ活性上升,但在较高浓度氨氮处理时,酶活性的诱导则受到抑制.说明适宜浓度的氨氮处理可增强刺参的免疫力,从而减轻病菌感染对刺参造成的免疫功能损伤和提高刺参抗病力.刺参在感染病原菌假交替单胞菌(Pseudoalte-romonas sp)的条件下,3 mg/L、4 mg/L和6 mg/L浓度的氨氮处理第6天,刺参的累积发病死亡率分别为44.4%、55.6%和72.2%,高于对照组,表明较高浓度的氨氮胁迫能够显著降低刺参的免疫力,增加对病原菌的易感性.因此,在刺参养殖过程中,氨氮浓度的调控具有重要的意义. 相似文献
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