Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition

Detoxification of synthetic dyes is one of the main challenges in clearing textile industry wastes. Biodegradation of azo-dyes using Phanerochaete chrysosporium is one the most environmentally friendly methods available. The main enzymes responsible for mycodecolorization process are lignin and manganese peroxidases. Here, optimization of expression conditions has been carried out with manipulating culture condition and nutrient sources. Therefore, the effects of buffer and temperature as well as nitrogen source on lignin peroxidase and manganese peroxidase production were investigated at two levels and four levels, respectively. For this purpose, P. chrysosporium RP78 based on Taguchi design of experiment has been applied. Maximum lignin and manganese peroxidase activities of 182 ± 2.5 U/L and 850 ± 41 U/L were obtained under predicted optimum conditions, respectively. Thereby, about 100 % decolorization was achieved after 24 h for two most widely used groups of azo dyes in textile industry consisting reactive and acidic. The physical adsorption of the azo dyes by mycelia was not significant which indicated that the enzymatic degradation of the dyes was occurred. Time profile of these enzymes showed that manganese peroxidase was peaked on 9^th day while lignin peroxidase peaked on 13^th day and remained stable in the culture. The extracellular expression profiles of both were studied by 2 dimensional gel electrophoresis to partially characterize the enzymes.     

Flood intensity and land evaluation in the Cauvery delta,Tamil Nadu,applying modern remote sensing techniques

An area of about 7500 km² covering Cauvery delta has been studied from airphotos (1:60,000), Landsats (1:250,000) and survey of India topographic sheets (1:50,000) with a view to delineate various landforms, soils, land use, hydromorphic units, drainage, and flood intensity of the delta. The delta is composed of mainly ancient channels, delta p ains, fossil islands, levees, beach ridges, mangroves marshes and coastal dunes. The various soil types are clayey soils, silty soils, silty loams, sandy loams, coastal sands and alkaline clays. Paddy and sugarcane are dominant crops. The old channels are found to be zones of high ground water potential. The growth of delta seems to be from south to north with twelve abandoned meander lobes and three delta fronts with gradual influx of material into Bay of Bengal. The old rivet courses lying parallel to the present active Coleroon river in third stage of the growth of the delta seem to be severely affected by floods. Moderate to low growth is noticeable in the second stage of the of the delta. The oldest river course which debouched into Bay of Bengal near Adirama-patnam is less affected by floods. The delta plains, fossil islands, disturbed ridges and beach ridges are unaffected. Basing on landforms, soils, land use, hydrogeomorphology and flood intensity the land capability of the delta has been evaluated. About seven classes of land are interpreted.     

Mercury flux to estuarine sediments, derived from Pb-210 and Cs-137 geochronologies (Guaratuba Bay, Brazil)

A sediment core from Guaratuba Bay was used to indicate possible Hg modifications to this coastal environment brought about by growing agricultural activity. Sedimentation rates were estimated to be 6.1 mm/year and 5.2 mm/year through 210Pb and 137Cs geochronologies, respectively. Mercury concentrations and organic matter ratios in the surface layers are greater than in the older sediments, supporting the hypothesis of anthropogenic enrichment. Results show that the Hg flux has raised more than twofold during the second half of the 20th century. These results point to the need for further studies to substantiate the hypothesis of anthropogenic enrichment and to quantify point sources of Hg to this estuary.     

Interception losses of rainfall from Cashew trees

The rainfall interception losses from Cashew trees were quantified, based on the records of 105 selected storms within the range 25.0 mm, occurring in a humid tropical region at Kottamparamba, India.

The storage capacity of the Cashew trees was worked out as 0.8 mm and the throughfall coefficient as 0.391. The trees under observation were 15–20 years of age with a leaf area index of 1.0–1.25.

About 31% of the storm rainfall for storms 25.0 mm was intercepted by the Cashew trees and lost to the atmosphere.

The measured interception losses from the trees were compared with the estimated interception losses using the analytical model of Gash (1979). The predicted interception losses from the Cashew trees were within ± 10% for storms with total rainfall 10.0 mm and within ± 22% for storms with a rainfall of 10.1–25.0 mm.     

Mangrove forest sedimentation and its reference to sea level rise,Cananeia, Brazil

The stability of a mangrove ecosystem in Cananeia, Brazil, is assessed based on investigations of the site-specific temporal rise in relative sea level during the past 50 years, 100-year sediment accumulation rates (SAR) and sources of organic matter (OM). Addressing this, three sediment cores were collected in a transect, intertidal mud flat, mangrove margin and well into the forest. The net SAR, as estimated by the age–depth relationships of ²¹⁰Pb and ¹³⁷Cs, is between 2.5 and 3.9 mm year⁻¹. These rates are comparable to the estimates based on the Pb and Zn contaminant markers corresponding to mining initiation in the region in 1918. Further, the SARs are lower than the rate of regional relative sea level rise (4 mm year⁻¹) as indicated by the past 50-year tide gauge record, but the rate is higher than the eustatic sea level rise (1.7 ± 0.3 mm year⁻¹). The stratigraphies of TOC/TN, δ¹³C_(OC), OP and δ¹⁵N indicate site-specific mangal vegetal litter, which is the predominant source of OM at all core sites, during the past century and reflects a stable mangal system over that time span.     

Polycyclic aromatic hydrocarbons in recent sediments from a subtropical estuary in Brazil

The scope of this work was to evaluate the levels of polycyclic aromatic hydrocarbons (PAHs) and their possible sources in two shallow sediment cores from an estuarine area located in the south region of Brazil, the Guaratuba Bay. The estuary is encompassed by an Environmental Protection Area (EPA) of 1306 km², which is still considered to be pristine, despite recent urban growth. To assess levels of 14 selected PAHs in the sediments, among those listed as “priority pollutants” by the US Environmental Protection Agency, the cores were divided in several sections, followed by extraction, clean-up, fractionation and subsequent analysis using HPLC coupled to a fluorescence detector. Among the PAHs detected, phenanthrene and fluoranthene showed the highest concentrations. PAHs with more than four rings were usually present in low concentrations or were undetectable. To assist in the identification of possible sources, the ratios An/(An + Phe) and Flt/(Flt + Py) (anthracene to anthracene plus phenanthrene and fluoranthene to fluoranthene plus pyrene) and a PCA model were also used. Total concentrations of PAHs ranged between 1.5 and 3130 ng/g (mean 495) dry weight for the core collected in the inner part of the estuary, and between 78.5 and 3270 ng/g (mean 899) dry weight for the other core, collected in the outer part. Compared to values found in other studies in coastal zones along South American eastern margin, this area can be considered to range from low polluted to moderately polluted. From the data, it was also possible to conclude that there is predominance of petroleum sources, and essentially close to the more urbanised areas. Combustion sources have only minor contribution and are episodic, when compared to the latter.