鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。     

Variational iteration solving method for El Nio phenomenon atmospheric physics of nonlinear model

1 IntroductionThe El Ni’o atmospheric physics oscillation is anabnormal phenomenon involved in the tropical Pacificocean- atmosphere interactions. Studies on the El Ni(ophenomenon are very attractive (Lin et al., 2000; Linet al., 2001, 2002; Wang, 2001; Feng et al., 2001;Feng et al., 2002; Liu et al., 2002; Wei and Chen,2003; Xie et al., 2002; Zhu et al., 2002; Pu et al.,2003; Gu et al., 2004; Yu and Liu, 2004). Lin and Mo(2004), Mo and Lin (2004) and Mo et al. (2004) alsostudied a s…     

Estimating the minimum in-stream flow requirements via wetted perimeter method based on curvature and slope techniques

Under the assumptions of triangular cross section channel and uniform stable flow, an analytical solution of the minimum ecological in-stream flow requirement (MEIFR) is deduced. Based on the analytical solution, the uncertainty of the wetted perimeter method is analyzed by comparing the two techniques for the determination of the critical point on the relationship curve between wetted perimeter, P and discharge, Q. It is clearly shown that the results of MEIFR based on curvature technique (corresponding to the maximum curvature) and slope technique (slope being 1) are significantly different. On the P-Q curve, the slope of the critical point with the maximum curvature is 0.39 and the MEIFR varied prominently with the change of the slope threshold. This indicates that if a certain value of the slope threshold is not available for slope technique, curvature technique may be a better choice. By applying the analytical solution of MEIFR in the losing rivers of the Western Route South-to-North Water Transfer Project in China, the MEIFR value via curvature technique is 2.5%-23.7% of the multi-year average annual discharge, while that for slope technique is 11%-105.7%. General conclusions would rely on the more detailed research for all kinds of cross-sections.     

贵州喀斯特山区墓地利用管理与耕地保护

通过对贵州亮岩峰丛山地的墓地进行随机抽样调查和统计分析,显示出墓地在一定坡度上的特定地段有集中趋势;在对墓地选址与土层厚度进行显著性检验后表明:喀斯特地区土层厚度著性影响墓地分布。以至于绝大多数墓地集中分布在日照较好、排水畅通、土层较厚的阳坡山麓地带,以占用土层较厚的优质耕地为主。在现实的墓地利用管理实践中,实施统一规划、集中管理,建立有偿使用制度、严格用地指标,加强墓地绿化,实行墓地园林化建设等措施,以期综合性多元化开发利用墓地,有效保护耕地资源。     

土地更新调查成果与二次调查数据衔接问题的探讨

结合广西第二次土地调查工作实际,论述了在调查工作中对原土地更新调查成果的利用,对需补充、完善的调查内容逐一进行了分析和阐述,并对如何处理两次调查数据衔接中存在的问题提出了一些建议和看法。     

滇西凤庆地区箐头山花岗岩的特征 ——古特提斯洋碰撞汇聚向伸展转换的证据

笔者等在野外调查的基础上,对出露于滇西临沧岩浆弧北部凤庆箐头山地区原1:25万凤庆县幅划分的二叠纪糜棱岩化黑云二长花岗岩进行了系统的年代学、岩石学和地球化学研究.该花岗岩体中,锆石具典型的振荡环带,Th/U值均大于0.2,为岩浆成因,其LA-ICP-MS U-Pb年龄为213±5 Ma,应当为岩浆结晶年龄,表明该岩体形...     

冈底斯带西段那木如岩体始新世岩浆作用及构造意义

冈底斯带西段狮泉河南部那木如岩体岩性变化较大,其中产出大量基性岩透镜体及暗色微细粒包体,空间上与花岗岩类呈渐变过渡接触关系。本文在详细野外调研的基础上,对狮泉河-札达一带那木如花岗岩及其中基性岩石进行了系统的岩石学、地球化学和同位素年代学研究。结果表明,那木如岩体主体岩性为黑云母花岗岩,其SiO₂为65%~76%,全碱含量较高,花岗岩中K₂O+Na₂O=5.50%~8.71%,基性岩石中则4.42%~6.7%。花岗岩类稀土元素最高含量为284.8×10^-6,最低只有105.4×10^-6;而基性岩类最高为120.4×10^-6,最低72.48×10^-6。两者稀土元素分配曲线均呈右倾平缓样式,花岗岩具有不明显Eu负异常,微量元素显示出花岗岩类和基性岩类具有相似的蛛网分布样式。两者均明显富K而亏损Nb、Ti等不活泼的HFS元素,显示出明显的岩浆混合作用趋势。4件花岗岩和基性岩样品所显示的LA-ICP-MS法锆石U-Pb年龄分别为46.11±0.78Ma、45.47±0.4Ma、46.7±2.9Ma和45.4±1.4Ma,变化在45.4~46.7Ma范围内,表明始新世早期(~46Ma)区域发生了岩浆混合作用。这一时限与冈底斯中、东部岩浆作用时代(40~52Ma)非常一致,表明始新世早期整个冈底斯发生了规模巨大的岩浆事件,暗示着印度-欧亚大陆碰撞作用在东西方向上所表现出的同时性。     

藏南沙拉岗锑矿流体包裹体红外显微测温研究

沙拉岗锑矿是藏南金锑成矿带内最具有代表性的锑矿床。为了更直接地了解该矿床的流体物理化学特征,本文利用红外显微镜对辉锑矿及紧密共生石英中的流体包裹体进行了显微测温研究。红外显微测温分析结果表明,辉锑矿中包裹体均一温度在134.9～221.9℃,峰值在160～190℃之间,盐度在1.7%～7.3%NaCleqv,峰值在5.0%～6.0%NaCleqv之间,密度在0.879～0.958g/cm3之间,平均值为0.934g/cm3;共生石英中包裹体均一温度在142.5～205.6℃,峰值在160～190℃之间,盐度在2.3%～7.0%NaCleqv,峰值在4.0%～6.0%NaCleqv之间,密度在0.910～0.947g/cm3之间,平均值为0.929g/cm3。通过对比研究认为沙拉岗辉锑矿及共生石英形成于同一物理化学条件,捕获同一成矿流体。结合石英中单个流体包裹体激光拉曼成分分析认为成矿流体为含微量CO2、N2、CH4气体的低温、低盐度和低密度的NaCl-H2O热液体系。成矿流体的沸腾作用是辉锑矿大量沉淀主要原因。     

金沙江-哀牢山断裂带几个富碱斑岩体的锆石U-Pb定年及地质意义

运用LA-ICP-MS锆石U-Pb同位素定年方法测定了金沙江-哀牢山断裂带内4个富碱斑岩的年龄.结果表明:剑川地区角闪正长斑岩形成时间为(37.6±2.2)Ma,姚安地区黑云母二长斑岩具有2组锆石U-Pb年龄数据,较年轻的一组为(36.86±0.63)Ma,较老的一组为(40.41±0.24)Ma,姚安黑云母角闪正长斑...     

Timing of Magma Mixing in the Gangdisě Magmatic Belt during the India-Asia Collision： Zircon SHRIMP U-Pb Dating

Abstract  Abundant mafic microgranular enclaves (MMEs) extensively distribute in granitoids in the Gangdisê giant magmatic belt, within which the Qüxü batholith is the most typical MME‐bearing pluton. Systematic sampling for granodioritic host rock, mafic microgranular enclaves and gabbro nearby at two locations in the Qüxü batholith, and subsequent zircon SHRIMP II U‐Pb dating have been conducted. Two sets of isotopic ages for granodioritic host rock, mafic microgranular enclaves and gabbro are 50.4±1.3 Ma, 51.2±1.1 Ma, 47.0±1 Ma and 49.3±1.7 Ma, 48.9±1.1 Ma, 49.9±1.7 Ma, respectively. It thus rules out the possibilities of mafic microgranular enclaves being refractory residues after partial melting of magma source region, or being xenoliths of country rocks or later intrusions. Therefore, it is believed that the three types of rocks mentioned above likely formed in the same magmatic event, i.e., they formed by magma mixing in the Eocene (c. 50 Ma). Compositionally, granitoid host rocks incline towards acidic end member involved in magma mixing, gabbros are akin to basic end member and mafic microgranular enclaves are the incompletely mixed basic magma clots trapped in acidic magma. The isotopic dating also suggested that huge‐scale magma mixing in the Gangdisê belt took place 15–20 million years after the initiation of the India‐Asia continental collision, genetically related to the underplating of subduction‐collision‐induced basic magma at the base of the continental crust. Underplating and magma mixing were likely the main process of mass‐energy exchange between the mantle and the crust during the continental collision, and greatly contributed to the accretion of the continental crust, the evolution of the lithosphere and related mineralization beneath the portion of the Tibetan Plateau to the north of the collision zone.