CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

福建省东部浅层地下水地球化学

本文对福建东部地区3.8万平方公里陆地浅层地下水(井水)1206件样品中19种元素的含量特征、元素间的相关关系、元素空间分布特点与地质背景的关系等方面进行了研究。研究表明,该地区浅层地下水中以Ba、Cl-、F-、Fe、Mn、Sr、Zn元素含量高、变化幅度大为显著特征;元素含量与pH没有明显的相关关系,Cl-、Sr、Co、Ni、Pb等元素间则具显著的正相关关系,Cl-具有决定性作用;地下水中元素的空间分布特征受地质背景及地理条件的制约。     

地下水数值模拟中人为边界的处理方法研究

边界条件预测是地下水数值模拟中的关键问题。本文针对人为边界受自然和人为因素影响,边界流量变化复杂难于预测的问题,讨论了人为边界条件的处理方式和实现方法,并将其应用到具有人为边界的二维潜水含水层系统。预测结果表明,受区内人工开采影响,边界流量呈逐年上升趋势,部分边界由流出边界转化为流入边界,结果符合地下水系统变化趋势,提高了预测精度。     

钱二块铀矿床采铀注液结垢趋势的理论分析与预测

用离子系数矩阵法对钱二块铀矿床地浸采铀试验注液中存在的独立离子反应进行了确定,由溶度积规则及反应平衡原理,借助Matlab编程,估算出了钱二块铀矿床地浸采铀试验注液可能会生成的沉淀物种类及数量,为防治结垢提供了一定的理论依据.     

GAS参数优化方法在黄河上游非汛期径流预报中的应用

本文采用相关分析与GAS参数优化相结合的方法,对黄河上游非汛期径流预报模型进行了研究,并对2000～2003年的径流进行了实时预报检验,从检验结果来看,这种预报方法具有较高的预报精度.本文为非汛期径流预报研究提供了参考和借鉴,同时,就计算分析手段而言,GAS在参数优化方面具有广泛的应用价值.     

川西前陆盆地中—新生代沉积迁移与构造转换

川西前陆盆地中—新生代各构造层的残余厚度展布和沉积特征分析发现,四川克拉通周缘的前陆盆地在晚三叠世时期发育于龙门山山前,明显属于龙门山褶皱逆冲构造载荷所形成的前渊凹陷;侏罗纪早期的沉积地层呈面状分布,没有表现出显著的挠曲沉降,指示了一个构造相对平静的阶段;中侏罗世早期前渊凹陷迁移至龙门山北段和米仓山山前,前渊沉积从晚三叠世的北东向转换为近东西向,广泛的湖泊相沉积预示了前陆盆地的欠充填状态;中侏罗世中晚期,川西盆地沉降中心又迁移到大巴山山前,相应的挠曲变形又从近东西向转化为北西向,构成了大巴山的前渊凹陷;晚侏罗世—早白垩世时期,沉降中心再次回到米仓山山前,巨厚的前渊凹陷沉积指示了米仓山冲断带的主要活动时期;白垩纪末—古近纪的前渊凹陷则跃迁至雅安—名山地区。川西前陆盆地的同造山沉降中心以四川盆地中心为核心在西部和北部呈弧形迁移,沉积序列不断更替和叠加。中生界各构造层底界构造图显示现今的构造低部位位于川西北地区和川西南地区,在川西北地区均有东西走向的等值线分布,而川西南地区等值线走向则为北东-南西向。因此分析认为,晚侏罗世至早白垩世的构造变形可能控制了川西盆地现今的地层变形,形成了川西北地区的南北向构造挤压结构,而晚期的新生代构造变形则主要体现在川西盆地的西南部,形成北东-南西向的地层展布特征。     

西藏西南部拉昂错地幔橄榄岩的地球化学特征及其构造意义

拉昂错蛇绿岩位于西藏西南部雅鲁藏布缝合带(YZSZ)的西段,由地幔橄榄岩和侵入其中的基性岩墙组成.拉昂错地幔橄榄岩普遍发育碎斑结构及熔体注入和交代结构,尖晶石的Cr#值具有较广泛的变化(0.32～0.70),大多数样品富集LREE并伴随HFSE的明显增加,少数亏损LREE,前者部分熔融程度为15%～23%,后者为10%左右,这表明它们并不是地幔单阶段部分熔融的残余物,而是MORB型亏损橄榄岩在俯冲过程中再度部分熔融后熔体与残余地幔相互作用的产物,由于熔体不同程度的混合与交代,形成了各种再饱满程度不同的橄榄岩.对拉昂错地幔橄榄岩岩石学和地球化学特征的研究,为探讨YZSZ蛇绿岩带所代表的特提斯洋盆的形成和演化提供了新的证据.     

烟台港和邻近锚地及其入境船舶压舱水中的浮游植物

本文根据2003年5月到2004年4月间烟台港和邻近锚地水域及其船舶压舱水中的浮游植物调查资料,对浮游植物的群落结构和压舱水对其影响进行了初步研究。结果表明,烟台港和临近锚地的浮游植物群落主要由温带沿岸性物种组成,以硅藻为主,还有少量甲藻和金藻;对比船舶压舱水中浮游植物群落发现:压舱水中浮游植物群落和本地群落的结构有很大差异,有害物种在压舱水中出现的频率较高。     

96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究

用96孔酶标板法测量单尾中国对虾血淋巴上清液抗菌（Uα）应力和过氧化酶活性。实验表明，该法具有以下优点：1）可测定大批单尾对虾血淋巴上清液的抗菌活力，酚氧化酶活性；2）所需对虾血淋巴上清液微量化；3）用酶标仪读取酶的活性，减少了不同试验者人为读数时的误差；4）重复性高。     

Formation of Equilibrium Beach Profile of the Abandoned Yellow River Delta Coast in North Jiangsu

The abandoned Yellow River Delta coast is a typical erodible silty and muddy coast in China. The paper analyses the marine dynamic characteristics and the mechanism of beach erosion of this area. Analysis and calculation show that in this sea area wave and tidal current action should be considered. Based on the above analysis, an equilibrium beach profile calculation model is developed, in which the wave-current interaction is considered while sediment supply and sediment re-deposition are neglected. The model consists of four parts: (1) calculation of wave parameters, (2) calculation of velocity due to wave-current interaction at different water depth, (3) calculation of friction velocity and shear stress at different water depths, and (4) calculation of the amount of sediment erosion, erosion intensity and variation of beach profile. Calculated results are in good agreement with observed data. Finally, the evolution tendency is discussed and the equilibrium beach profile of this coast is calculated. B