Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

基于GPIB接口的计数器数据采集与控制软件

在Windows平台上 ,使用VC 6 .0开发了一套基于GPIB接口的计数器数据采集与控制软件 ,其特点是采用了多线程机制 ,可实现对计数器的控制 ,以及数据的采集与实时显示。     

青藏高原隆升的非线性动态有限元仿真研究

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

“BASS 300 PARACOM”: a “model” underwaterparametric communication system

Parametric transduction offers valuable advantages for underwater acoustic communications. Perhaps the most significant benefit is the fact that high directivity is achieved by means of a physically small transmit transducer. This feature may, ultimately, be employed to permit long-range, low-frequency communication using a compact source. The high directivity is desirable to combat multipath propagation and to achieve data communications in water which is shallow by comparison with range. A real-time, high data-rate “model” differential phase shift keying (DPSK) communication system has been constructed and demonstrated. This system uses parametric transduction, with a 300-kHz primary frequency and a 50-kHz secondary frequency. Experimental results show that the system can be employed to combat multipath propagation in shallow water and can achieve high data-rate text and color image transmission at 10 and 20 kb s^-1 for 2-DPSK and 4-DPSK, respectively, through a transmission bandwidth of 10 kHz. The “model” system was developed to confirm performance predictions for a future, operational long-range link employing a 50-kHz primary frequency and a 5-kHz secondary frequency     

Geochemical survey of thermal system in Northeast China and their geothermometric characters

The chemical composition of several thermal springs around Changbaishan area has been investigated. Cenozoic basaltic rocks are widely distributed in Northeast China and geothermal characteristics have been described. About one hundred hot springs exist around Changhaishan Volcano at the border between China and D.P.R.Korea with high temperature about 82~C. The pH values of the spring water range from 6.9 to 7.1 and the total flow rate is about 4.8 L/sec. The chemical composition of the thermal springs is sodium carbonate; the high-mineral contents of thermal water are believed to have medicinal properties. Bathhouses are already built along the hot springs to take the advantage of the supposed healing properties. The high quality of those hot springs is believed to be utilitized for mineral water. The chemical equilibrium temperatures were estimated at about 160℃ based on the Na-K-Ca geochemical thermometer.     

Dust supply from river floodplains: the case of the lower Huang He (Yellow River) recorded in a loess–palaeosol sequence from the Mangshan Plateau

The Mangshan Plateau is located on the south bank of the Huang He (Yellow River) just west of the city of Zhengzhou, well outside the Loess Plateau in central China. Mixing models of the grain‐size data indicate that the loess deposits are mixtures of three loess components. Comparison of the mixing model with existing models established for a series of loess–palaeosol sequences from the Loess Plateau indicates that the Mangshan loess has been supplied from a proximal dust source, the Huang He floodplain, during major dust outbreaks. The high accumulation rates, the composition of the loess components, and especially the high proportions of a sandy loess component support this. Owing to the exceptionally high accumulation rates, the Mangshan grain size, magnetic susceptibility and carbonate records provide a high‐resolution archive of environmental and climate change. Copyright © 2008 John Wiley & Sons, Ltd.     

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica     

地壳对海洋潮汐的响应

应用三维动态有限元方法研究了中国北部地区的地壳对邻近的渤海与黄海海平面变化的响应。虽然此应力场过于微弱不足以引发地震，但发现应力集中的位置及应力场变化较大的位置恰好与某些现代地震的震中一致。这一结果表明研究地壳对广泛分布的载荷的响应对研究区域地震构造是有帮助的。     

Evolution of Electric Currents Associated with Two M-Class Flares

Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare.