Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

Mirdita Zone ophiolites and associated sediments in Albania reveal Neotethys Ocean origin

The Mirdita Ophiolite Zone in Albania is associated with widespread mélanges containing components of up to nappe-size. We dated matrix and components of the mélange by radiolarians, conodonts, and other taxa. The components consist of radiolarites, pelagic limestones and shallow-water limestones, all of Triassic age, as well as ophiolites. Triassic radiolarite as a primary cover of ophiolite material proves Middle Triassic onset of Mirdita ocean-floor formation. The mélange contains a turbiditic radiolarite-rich matrix (“radiolaritic flysch”), dated as Late Bajocian to Early Oxfordian. It formed as a synorogenic sediment during west-directed thrusting of ophiolite and sediment-cover nappes representing ocean floor and underplated fragments of the western continental margin. The tectonic structures formed during these orogenic events (“Younger Kimmeridian or Eohellenic Orogeny”) are sealed by Late Jurassic platform carbonates. The geological history conforms with that of the Inner Dinarides and adjoining areas; we therefore correlate the Mirdita-Pindos Ophiolite Zone with the Vardar Zone and explain its present position by far-distance west-directed thrusting.     

Cretaceous transformation from passive to active continental margin in the Western Carpathians as indicated by the sedimentary record in the Infratatric unit

The Cretaceous orogen of the Western Carpathians comprises fragments of the destructed northern Centrocarpathian domain, which is defined as Infratatric unit and formed a continental margin facing the Penninic Ocean in Jurassic and Cretaceous times. The breakup event and opening of the Penninic Ocean occurred in the Early Jurassic (Pliensbachian), which is recorded by an abrupt deepening event from shallow-water sediments to deep-water nodular limestone in the Infratatric sediment succession. The transformation of the passive into an active continental margin by the onset of subduction of the Penninic oceanic crust occurred in Santonian times and is reflected by the beginning of flysch deposition in the Infratatric Belice domain, which took the position of a forearc basin in the convergent margin setting. The forearc basin was supplied by clastic material from the more internal part of the Infratatric unit, which experienced nappe stacking, metamorphism, and subsequent exhumation in Late Cretaceous times. In the frontal part of the forearc basin an accretionary wedge was built up, which formed an outer-arc ridge and delivered detrital material into the forearc basin in Maastrichtian time. Final collision between the European and the Adriatic plate occurred in the Eocene period and is responsible for weak metamorphism in the Infratatric unit.     

Berriasian drowning of the Plassen carbonate platform at the type-locality and its bearing on the early Eoalpine orogenic dynamics in the Northern Calcareous Alps (Austria)

The Plassen carbonate platform (Kimmeridgian to Early Berriasian) developed above the Callovian to Tithonian carbonate clastic radiolaritic flysch basins of the Northern Calcareous Alps during a tectonically active period in a convergent regime. Remnants of the drowning sequence of the Plassen Formation have been discovered at Mount Plassen in the Austrian Salzkammergut. It is represented by calpionellid-radiolaria wacke- to packstones that, due to the occurrence of Calpionellopsis oblonga (Cadisch), are of Late Berriasian age (oblonga Subzone). Thus, the Plassen Formation at its type-locality shows the most complete profile presently known, documenting the carbonate platform evolution from the initial shallowing upward evolution in the Kimmeridgian until the final Berriasian drowning. The shift from neritic to pelagic sedimentation took place during Berriasian times. A siliciclastic-influenced drowning sequence sealed the highly differentiated Plassen carbonate platform. The former interpretation of a Late Jurassic carbonate platform formed under conditions of tectonic quiescence cannot be confirmed. The onset, evolution and drowning of the Plassen carbonate platform took place at an active continental margin. The tectonic evolution of the Northern Calcareous Alps during the Kimmeridgian to Berriasian time span and the reasons for the final drowning of the Plassen carbonate platform are to be seen in connection with further tectonic shortening after the closure of the Tethys Ocean.     

Sequence and isotope stratigraphy of Late Triassic distal periplatform limestones from the Northern Calcareous Alps (Kälberstein Quarry, Berchtesgaden Hallstatt Zone)

Received: 24 September 1998 / Accepted: 14 November 1999     

The change from rifting to thrusting in the Northern Calcareous Alps as recorded in Jurassic sediments

Facies analysis, fossil dating, and the study of the metamorphism in the Late Triassic to Early Cretaceous sedimentary successions in the central part of the Northern Calcareous Alps allow to reconstruct the tectonic evolution in the area between the South Penninic Ocean in the northwest and the Tethys Ocean with the Hallstatt Zone in the southeast. The Triassic as well as the Early and Middle Jurassic sediments were deposited in a rifted, transtensive continental margin setting. Around the Middle/Late Jurassic boundary two trenches in front of advancing nappes formed in sequence in the central part of the Northern Calcareous Alps. The southern trench (Late Callovian to Early Oxfordian) accumulated a thick succession of gravitatively redeposited sediments derived from the sedimentary sequences of the accreted Triassic–Liassic Hallstatt Zone deposited on the outer shelf and the margin of the Late Triassic carbonate platform. During a previous stage these sediments derived from sequences deposited on the more distal shelf (Salzberg facies zone of Hallstatt unit, Meliaticum), and in a later stage from more proximal parts (Zlambach facies zone of Hallstatt unit, Late Triassic reef belt). Low temperature–high pressure metamorphism of some Hallstatt limestones before redeposition is explained by the closure of parts of the Tethys Ocean in Middle to Late Jurassic times and associated subduction. In the northern trench (Late Oxfordian to Kimmeridgian) several hundred meters of sediment accumulated including redeposited material from a nearby topographic rise. This rise is interpreted as an advancing nappe front as a result of the subduction process. The sedimentary sealing by Tithonian sediments, documented by uniform deep-water sedimentation (Oberalm Formation), gives an upper time constraint for the tectonic events. In contrast to current models, which propose an extensional regime for the central and eastern Northern Calcareous Alps in the Late Jurassic, we propose a geodynamic model with a compressional regime related to the Kimmerian orogeny.     

Palaeomagnetism of three dyke swarms in Nansen Land,north Greenland (83° N)

Three basic dyke swarms of post-Ellesmerian (post-Early Carboniferous) age in Nansen Land (83° N, 43° W) are still not dated numerically, but cross-cutting relationships show Group 1 to be older than Group 2, while Group 3 is the freshest and likely the youngest. Group 1 (the most northerly swarm) strikes N-S; Group 2 NW-SE, and Group 3 (the most southerly swarm) E-W. From more than 200 dykes 234 specimens from 28 sites were investigated palaeomagnetically. Group 1 dykes show unexpected shallow inclinations with a cleaned mean direction of (Dm, Im) = (151°, –5.8°), N = 7, k = 18.5, ₉₅ = 13.9°. They show hydrothermal alterations, some remagnetization by lightning, and the low inclination indicates a low palaeo latitude. The palaeopole is (Plat, Plon) = (8.9° S, 14.0° W) with (dp, dm) = (7°, 14°), and is close to the North American Early Carboniferous mean pole, suggesting a syn- or early late-tectonic dyke injection. The polarity is reverse. Groups 2 and 3 of presumed Cretaceous or Tertiary age show dominantly normal and reverse polarities, respectively. Their mean directions per polarity are well grouped, with (Dm, Im) = (–30.6°, 76.7°), n = 13, k = 191.4, ₉₅ = 3.9°, and (Dm, Im) = (133.4°, –76.7°), n = 10, k = 87.5, ₉₅ = 5.9°, respectively. They are antipodal within 95% significance, and combining both swarms gives (Dm, Im) = (–37.5°, 76.8°), n = 23, k = 124.3, ₉₅ = 2.7°, corresponding to a mean pole of (Plat, Plon) = (70.0° N, 185.1° E) with (dp, dm) = (4.7°, 5.0°), for which the spline of Late Cretaceous-Tertiary poles for all Greenland indicates a palaeomagnetic age of 57 ± 10 Ma. This pole (in present-day coordinates) is very close to the Late Cretaceous North American pole, in accordance with the fact that Greenland belongs to the North American craton, and that the two younger swarms are essentially postdating the opening of Baffin Bay.