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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
2.
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk
thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only
the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated
fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs
was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae,
and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs
or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments
containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally
as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it
was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage
of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible
for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of
the starfish had already regionalized before the GV break-down.
Contribution No. 1722 from the Institute of Oceanology, Academia Sinica 相似文献
3.
Y.-B. WU S. GAO H.-J. GONG H. XIANG W.-F. JIAO S.-H. YANG Y.-S. LIU H.-L. YUAN 《Journal of Metamorphic Geology》2009,27(6):461-477
U–Pb age, trace element and Hf isotope compositions of zircon were analysed for a metasedimentary rock and two amphibolites from the Kongling terrane in the northern part of the Yangtze Craton. The zircon shows distinct morphological and chemical characteristics. Most zircon in an amphibolite shows oscillatory zoning, high Th/U and 176 Lu/177 Hf ratios, high formation temperature, high trace element contents, clear negative Eu anomaly, as well as HREE-enriched patterns, suggesting that it is igneous. The zircon yields a weighted mean 207 Pb/206 Pb age of 2857 ± 8 Ma, representing the age of the magmatic protolith. The zircon in the other two samples is metamorphic. It has low Th/U ratios, low trace element concentrations, variable HREE contents (33.8 ≥ LuN ≥2213; 14.7 ≤ LuN /SmN ≤ 354) and 176 Lu/177 Hf ratios (0.000030–0.001168). The data indicate that the zircon formed in the presence of garnet and under upper amphibolite facies conditions. The metamorphic zircon yields a weighted mean 207 Pb/206 Pb age of 2010 ± 13 Ma. These results combined with previously obtained Palaeoproterozoic metamorphic ages suggest a c. 2.0 Ga Palaeoproterozoic collisional event in the Yangtze Craton, which may result from the assembly of the supercontinent Columbia. The zircon in two samples yields weighted mean two-stage Hf model ( T DM2 ) ages of 3217 ± 110 and 2943 ± 50 Ma, respectively, indicating that their protoliths were mainly derived from Archean crust. 相似文献
4.
The time evolution behaviour of the superhumps of the dwarf nova1RXS J232953.9+ 062814 is investigated with the wavelet analysis
method. On the basis of two nights CCD photometry performed during its first superoutburst as well as other published brightness
data, we reveal the superhump's time-dependence as a function of periods and time. Our light curves, which phased in the rapid
decay ending portion of the superoutburst and in the dawn of a following normal outburst, are important to help trace the
superhump evolution for the star. Both the superhump period and the orbital period of the binary system are detected in the
present data. We obtain P
sh=0.0458±0.0002d and P
orb=0.0450±0.0002d. They agree with those existing values. The two periods exchanged their roles during the superhump evolution.
The general profile of brightness fading over the outbursts roughly followed an exponential decay law or a form of a five-order
polynomial.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
5.
It is well known that the Babuska–Brezzi stability criterion or the Zienkiewicz–Taylor patch test precludes the use of the finite elements with the same low order of interpolation for displacement and pore pressure in the nearly incompressible and undrained cases, unless some stabilization techniques are introduced for dynamic analysis of saturated porous medium where coupling occurs between the displacement of solid skeleton and pore pressure. The numerical manifold method (NMM), where the interpolation of displacement and pressure can be determined independently in an element for the solution of u–p formulation, is derived based on triangular mesh for the requirement of high accurate calculations from practical applications in the dynamic analysis of saturated porous materials. The matrices of equilibrium equations for the second‐order displacement and the first‐order pressure manifold method are given in detail for program coding. By close comparison with widely used finite element method, the NMM presents good stability for the coupling problems, particularly in the nearly incompressible and undrained cases. Numerical examples are given to illustrate the validity and stability of the manifold element developed. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
6.
四川石棉大水沟独立碲矿床堪称世界首例,其矿体两侧的围岩蚀变较窄,且蚀变带与矿脉及未蚀变岩呈清楚而截然的接触关系。主要围岩蚀变为白云石化、白(绢)云母化、云英岩化及电气石化等。这些蚀变作用分别发生于177.7~165.1Ma和91.71~80.19Ma。蚀变作用过程中SiO2大量析出,Al2O3、TiO2、CaO、MgO、K2O、H2O、CO2、Fe2O3、MnO则有不同程度地带入。与此同时,REE在此过程中包集体迁出,Te、Bi、AS等元素的含量则决定于蚀变作用及其强度,而与原岩无关。 相似文献
7.
月日潮汐摩擦和地球惯量矩变化是日长长期变化的主要原因.在本文中,利用最新的地球物理和古生物钟数据,对过去15亿年以来的月日潮汐摩擦、地球惯量矩变化和日长长期变化等作了数值对比研究.由此得到二个重要结论:一是仅利用地球的自转形变不能解释J2的变化,这说明地球的重力分异现象至今仍存在着;其二是在几亿年前的潮汐摩擦比现在大得多,若取潮汐耗散与距离的立方成反比时,理论结果与由古生物钟得到的回归年日数和朔望月日数数据较为符合。 相似文献
8.
9.
STUDY ON GIS FOR YIELD ESTIMATION BY REMOTE SENSING IN JILIN MAIZE BELTSTUDYONGISFORYIELDESTIMATIONBYREMOTESENSINGINJILINMAIZ... 相似文献
10.