Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica     

Zircon U–Pb age, trace element and Hf isotope composition of Kongling terrane in the Yangtze Craton: refining the timing of Palaeoproterozoic high-grade metamorphism

U–Pb age, trace element and Hf isotope compositions of zircon were analysed for a metasedimentary rock and two amphibolites from the Kongling terrane in the northern part of the Yangtze Craton. The zircon shows distinct morphological and chemical characteristics. Most zircon in an amphibolite shows oscillatory zoning, high Th/U and ¹⁷⁶Lu/¹⁷⁷Hf ratios, high formation temperature, high trace element contents, clear negative Eu anomaly, as well as HREE-enriched patterns, suggesting that it is igneous. The zircon yields a weighted mean ²⁰⁷Pb/²⁰⁶Pb age of 2857 ± 8 Ma, representing the age of the magmatic protolith. The zircon in the other two samples is metamorphic. It has low Th/U ratios, low trace element concentrations, variable HREE contents (33.8 ≥ Lu_N≥2213; 14.7 ≤ Lu_N/Sm_N ≤ 354) and ¹⁷⁶Lu/¹⁷⁷Hf ratios (0.000030–0.001168). The data indicate that the zircon formed in the presence of garnet and under upper amphibolite facies conditions. The metamorphic zircon yields a weighted mean ²⁰⁷Pb/²⁰⁶Pb age of 2010 ± 13 Ma. These results combined with previously obtained Palaeoproterozoic metamorphic ages suggest a c. 2.0 Ga Palaeoproterozoic collisional event in the Yangtze Craton, which may result from the assembly of the supercontinent Columbia. The zircon in two samples yields weighted mean two-stage Hf model ( T _DM2) ages of 3217 ± 110 and 2943 ± 50 Ma, respectively, indicating that their protoliths were mainly derived from Archean crust.     

A Time Evolution Study of the Superhumps of the Dwarf Nova 1RXS J232953.9+062814 by Wavelet Transform

The time evolution behaviour of the superhumps of the dwarf nova1RXS J232953.9+ 062814 is investigated with the wavelet analysis method. On the basis of two nights CCD photometry performed during its first superoutburst as well as other published brightness data, we reveal the superhump's time-dependence as a function of periods and time. Our light curves, which phased in the rapid decay ending portion of the superoutburst and in the dawn of a following normal outburst, are important to help trace the superhump evolution for the star. Both the superhump period and the orbital period of the binary system are detected in the present data. We obtain P _sh=0.0458±0.0002d and P _orb=0.0450±0.0002d. They agree with those existing values. The two periods exchanged their roles during the superhump evolution. The general profile of brightness fading over the outbursts roughly followed an exponential decay law or a form of a five-order polynomial. This revised version was published online in July 2006 with corrections to the Cover Date.     

Numerical manifold method for dynamic nonlinear analysis of saturated porous media

It is well known that the Babuska–Brezzi stability criterion or the Zienkiewicz–Taylor patch test precludes the use of the finite elements with the same low order of interpolation for displacement and pore pressure in the nearly incompressible and undrained cases, unless some stabilization techniques are introduced for dynamic analysis of saturated porous medium where coupling occurs between the displacement of solid skeleton and pore pressure. The numerical manifold method (NMM), where the interpolation of displacement and pressure can be determined independently in an element for the solution of u–p formulation, is derived based on triangular mesh for the requirement of high accurate calculations from practical applications in the dynamic analysis of saturated porous materials. The matrices of equilibrium equations for the second‐order displacement and the first‐order pressure manifold method are given in detail for program coding. By close comparison with widely used finite element method, the NMM presents good stability for the coupling problems, particularly in the nearly incompressible and undrained cases. Numerical examples are given to illustrate the validity and stability of the manifold element developed. Copyright © 2006 John Wiley & Sons, Ltd.     

四川石棉独立碲矿床蚀变岩的地质地球化学研究

四川石棉大水沟独立碲矿床堪称世界首例，其矿体两侧的围岩蚀变较窄，且蚀变带与矿脉及未蚀变岩呈清楚而截然的接触关系。主要围岩蚀变为白云石化、白（绢）云母化、云英岩化及电气石化等。这些蚀变作用分别发生于１７７．７～１６５．１Ｍａ和９１．７１～８０．１９Ｍａ。蚀变作用过程中ＳｉＯ２大量析出，Ａｌ２Ｏ３、ＴｉＯ２、ＣａＯ、ＭｇＯ、Ｋ２Ｏ、Ｈ２Ｏ、ＣＯ２、Ｆｅ２Ｏ３、ＭｎＯ则有不同程度地带入。与此同时，ＲＥＥ在此过程中包集体迁出，Ｔｅ、Ｂｉ、ＡＳ等元素的含量则决定于蚀变作用及其强度，而与原岩无关。     

对月日潮汐摩擦耗散的估计

月日潮汐摩擦和地球惯量矩变化是日长长期变化的主要原因．在本文中，利用最新的地球物理和古生物钟数据，对过去15亿年以来的月日潮汐摩擦、地球惯量矩变化和日长长期变化等作了数值对比研究．由此得到二个重要结论：一是仅利用地球的自转形变不能解释J2的变化，这说明地球的重力分异现象至今仍存在着；其二是在几亿年前的潮汐摩擦比现在大得多，若取潮汐耗散与距离的立方成反比时，理论结果与由古生物钟得到的回归年日数和朔望月日数数据较为符合。     

海量多波段星表数据的交叉证认的实现

许多天文学家需要星表来选源、证认和研究。目前国外有一些大的数据中心提供了星表查询、多源查询(即小样本交叉证认)、可视化等功能。但是随着大数据量及全波段天文时代的到来,这些服务已经越来越不能满足天文学家的需求。在详细分析了国外数据中心的服务后,提出了新的问题,并给出一套解决方案。着重讨论了任意两个星表的交叉证认和大数据量星表的交叉证认的实现,以及交叉证认结果的分类和参数的自由选择等方面的课题。     

STUDY ON GIS FOR YIELD ESTIMATION BY REMOTE SENSING IN JILIN MAIZE BELT

ＳＴＵＤＹ　ＯＮ　ＧＩＳ　ＦＯＲ　ＹＩＥＬＤ　ＥＳＴＩＭＡＴＩＯＮ　ＢＹ　ＲＥＭＯＴＥ　ＳＥＮＳＩＮＧ　ＩＮ　ＪＩＬＩＮ　ＭＡＩＺＥ　ＢＥＬＴＳＴＵＤＹＯＮＧＩＳＦＯＲＹＩＥＬＤＥＳＴＩＭＡＴＩＯＮＢＹＲＥＭＯＴＥＳＥＮＳＩＮＧＩＮＪＩＬＩＮＭＡＩＺ...