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1.
Diagnosis of iridovirus in large yellow croaker by PCR   总被引:1,自引:0,他引:1  
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   
2.
INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…  相似文献   
3.
Developing ecotourism in First World, resource-dependent areas   总被引:1,自引:0,他引:1  
Deborah Che 《Geoforum》2006,37(2):212-226
Ecotourism, an economic diversification tool most commonly applied in the Third World as a means to protect ecosystems, preserve local cultures, and spur economic development, has recently been applied in First World resource-dependent areas. While ecotourism has traditionally focused on Third World ‘undisturbed’ protected lands, it has also been developed in their First World equivalents (i.e., old-growth forests) as well as in First World sites of past resource extraction and in places where current agricultural practices maintain cherished cultural landscapes. Forest County, Pennsylvania, a timber-dependent area, sought to diversify its economy by developing ecotourism based on its unique Allegheny hardwood forests, which are produced by timber harvesting. This ecotourism would encourage amenity-based, locally-driven economic development and maintain timber harvesting. While government and foundation supported ecotourism development efforts in areas dependent on resource extraction have incorporated some of ecotourism’s ideals, these operations have had mixed success. Such isolated areas, which have traditionally drawn visitors independently engaging in traditional outdoor recreation activities, have not been able to draw enough customers willing to pay for natural and cultural history tours. If ecotourism is to be successful, such areas may need further government support and destination branding to increase name recognition in order to counter the global orientation of the nature tour industry. For true community development, local collaborative efforts including resource and environmental interests are also required in which primary production is connected to processing and consumers through value-added and service sector activities such as tourism.  相似文献   
4.
A detrital zircon aged 4.1 Ga is discovered by the SHRIMP U-Pb method in a quartzite in Burang County, western Tibet. This is presently the oldest single-grain detrital zircon in China. The Th-U ratios of the two testing points of the 〉4.0 Ga zircon are between 0.76 and 0.86, indicating their magmatic origin. This discovery has offered an important age for investigating the geological evolution of the Qinghai-Tibet Plateau.  相似文献   
5.
从井孔周围地下水开采量变化、大气降雨补给量变化、农田灌溉用水量变化、矿井塌方、涌水、排水和地震活动等影响因素入手 ,对 1999年至 2 0 0 0年间介休井水位的 2次突降原因进行了调查。通过初步分析 ,表明介休井水位阶降型异常具有一定的震兆性质 ,其水位的缓慢上升与突然阶降意味着介休地区断裂活动在加速。其地震预测的意义是未来一段时间山西地震带内有发生MS≥ 5 0级地震可能  相似文献   
6.
云南丽江地区断裂构造岩岩组动力学研究   总被引:2,自引:0,他引:2  
丽江地区构造岩岩组动力学研究表明,研究区内中更新世末构造主压应力保持在北西至北西西方向变化;晚更新世中期之后构造主压应力方向则以北北东至北东方向为主变化,并有逐渐向近南北向转化的特点。因此玉龙雪山东麓断裂在中更新世末曾有过左旋压扭活动为主的历史,兼有左旋、右旋的活动过程,1996年2月3日丽江M7.0地震的破裂过程继承了晚更新世后期断裂的活动特点。  相似文献   
7.
提出依据开挖剖面中的楔形体在受到断层作用时其厚度的变化量求解断层水平位移的方法。分析表明断层水平位移量S与楔形体在断层两侧厚度变化量Δh满足关系式S=Δh/ρsinβ。式中ρ为楔形体在断层走向上的尖灭系数,β为断层的倾角,该关系式不仅能满足纯走滑运动的断层,而且也满足一般斜滑断层,同时提出了在剖面上判断断层水平运动性质的方法。  相似文献   
8.
A lot of slow fluctuations of water level have been observed in the original recording maps of subsurface fluid in Well Shuozhou, Shanxi Province. Some typical recording maps of the “precursors” are introduced in the paper and the features of the “precursors” recorded by the well are analyzed. The results show that 38% strong earthquakes possess this kind of record, which has a fluctuation period arranging from several to tens of minutes and appear mostly two days before the earthquakes. Moreover, the mechanism, transmission and responding conditions of well water level are discussed, as well as the scientific meaning and practical value of the “precursor” of the well water level in earthquake predictions. Foundation item: State Natural Science Foundation of China (19973011).  相似文献   
9.
根据库仑静应力变化的概念,对青藏块体中部地区1997年11月8日玛尼7.9级地震及2001年11月14日昆仑山口西8.1级地震的应力触发作用进行了初步研究。不同震源机制解计算结果表明,玛尼地震造成了昆仑地震断层面上10^-3MPa量级的库仑应力增量,该变化仅使得昆仑地震的发生提前了最长10年左右,表明当前地壳应变能水平可能并不很低。对照20世纪初以来全球7级以上强震频度和强度演化特征与地球自转变化的关系,认为地球自转幅度衰减、变化频率升高是近期强震活动的重要控制因素。昆仑山口西8.1级地震的发生,可能是地球自转持续加速阶段后期,青藏块体内部区域应力场逐渐强化,及玛尼地震应力触发的结果。  相似文献   
10.
Based on Coulomb static stress variation, the stress trigging action of the Mani ( Ms7.9, Nov.1997) earthquake on the West Kuulun Mountains Pass (MsS.1, Nov. 2001 ) earthquake is researched. Results of different source mechanism resolutions show that a 10_3 MPa Coulomb stress due to the Mani earthquake was added to the fracture fault of the Kunlun earthquake, and this may shift the broken date to about 10 years earlier, and infer that the stress level in the crust is not low. Comparing the relationship between strong earthquake strength and frequency and earth rotation change, it is shown that the strength‘s decrease and the variation period‘ s shortening of earth rotation are important controlling factors on strong earthquake activity. This great event with Ms = 8.1 took place probably due to a gradual strengthening background of regional stress field within the Qinghai-Xizang block in the period of acceleration of change of day‘s length and stress triggering from the Mani earthquake.  相似文献   
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