深海细菌Pseudomonas sp. IOFA1中甲醛歧化酶FM11的纯化及性质研究

从印度洋深海沉积物中分离出一株能够高效降解甲醛的假单胞菌(Pseudomonas sp.IOFA1),该菌株可在40 min内完全降解100 mg/cm~3的甲醛.通过阴离子交换柱层析对菌株破碎液中的粗酶液进行纯化,得到一条具有甲醛降解活性的单一蛋白条带.经MALDI-TOF/TOF质谱鉴定,该蛋白为甲醛歧化酶蛋白(FM11).序列分析表明FM11与其它物种已知醛脱氢酶的氨基酸序列最高相似性为65%;结构域分析表明FM11含有2个保守的锌结合位点和一个NAD结合位点.酶学性质分析结果显示,FM11的最适反应温度为40℃,在25~50℃范围内能够保留95%以上的酶活力,尤其是在高于70℃的高温环境下仍保有约50%的活力,表现出广泛的温度适应性.FM11具有非常广泛的最适反应pH值范围(pH值为5~9),且在pH值4和pH值10时仍分别保留50%和80%左右的酶活力,表现出广泛的pH适应性.在pH值为5~7的条件下孵育1 h后,FM11仍保有97%以上的酶活,且在pH值为7~10条件下孵育1 h后,仍保留90%以上的酶活,表现出极好的酸性和碱性稳定性.Ca~(2+)、Mg~(2+)在低浓度时(0.5 mmol/dm~3)对FM11有较明显的激活作用.这些研究为我们进一步开发Pseudomonas sp.IOFA1甲醛歧化酶在降解甲醛方面的用途奠定了基础.     

关于2021年5月滇西漾濞MS6.4地震序列特征及成因的初步研究

2021年5月21日21时48分在滇西苍山西麓漾濞地区发生MS6.4(MW6.1)强震,相关地震活动表现为一个典型的前震-主震-余震序列.本研究分别就该地震序列的构造背景、M1.0以上地震的双差定位、主要地震的矩张量反演和破裂传播方向、应力场反演及断层滑动趋势以及潮汐作用等方面进行了初步分析.矩张量反演结果表明,矩心深...     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

A new fault rupture scenario for the 2003 Mw 6.6 Bam earthquake, SE Iran: Insights from the high-resolution QuickBird imagery and field observations

The December 26, 2003 M_w 6.6 Bam earthquake is one of the most disastrous earthquakes in Iran. QuickBird panchromatic and multispectral satellite imagery with 61 cm and 2.4 m ground resolution, respectively provide new insights into the surface rupturing process associated with this earthquake. The results indicate that this earthquake produced a 2–5 km-wide surface rupture zone with a complex geometric pattern. A 10-km-long surface rupture zone developed along the pre-existing Bam fault trace. Two additional surface rupture zones, each 2–5 km long, are oblique to the pre-existing Bam fault in angles of 20–35°. An analysis of geometric and geomorphic features also shows that movement on the Bam fault is mainly right-lateral motion with some compressional component. This interpretation is consistent with field investigations, analysis of aftershocks as well as teleseismic inversion. Therefore, we suggest that the 2003 Bam earthquake occurred on the Bam fault, and that the surface ruptures oblique to the Bam fault are caused by secondary faulting such as synthetic shears (Reidel shears). Our fault model for the Bam earthquake provides a new tectonic scenario for explaining complex surface deformations associated with the Bam earthquake.     

基于土地定级估价信息的市场比较法研究

探讨了在土地定级估价信息系统中，如何将市场比较法与基准地价体系有机地结合，充分利用土地定级和基准地价评估的成果进行宗地评估的技术和方法。通过模板建立并设计了相应的数据结构，解决了数据库结构的不确定性问题，采用聚类分析等数学方法实现了案例提取、匹配以及地价计算相应流程的算法。基于土地定级估价信息的市场比较法克服了传统市场比较法实现存在的问题，对实现宗地地价评估流程的自动化、保证评估结果的客观性具有重大意义。     

气候变暖背景下祁连山西部山区水循环要素的变化——以疏勒河干流上游山区为例

根据祁连山区西部托勒气象站与疏勒河上游昌马堡水文站、鱼儿红雨量站的气温、降水、径流等观测数据,对近50年来疏勒河山区流域气温、降水、径流等水循环要素的变化特征与趋势进行了分析.结果表明,近50年来祁连西部山区年平均气温呈持续上升的态势,并在1990年代中期后出现一个突变,突变后气温上升速率较突变前明显加快.从气温的季节变化上看,冬季升温的幅度明显大于其他各季,从气温的区域变化来看,中低山地带的气温升幅要大于中高山地带.分析结果还显示,祁连山区西部年降水量总体上亦呈增长的态势,但年际波动比较剧烈.少雨年主要在1990年代以前,多雨年在近20 a;从季节变化上看,夏季降水量变化比较稳定,增减趋势不明显,其他各季降水量均有明显的上升趋势,冬季降水量增幅明显.受降水与气温加速上升所带来的冰雪融水增加的影响,疏勒河出山径流的年平均与四季流量亦呈显著增加的态势.考虑到山区夏季降水并未增加,故占年径流量比重较大的夏季径流量的增加主要是冰雪融水的贡献.     

滇西南澜沧江带红豆山铜矿床蚀变分带及其找矿意义

红豆山铜矿床是南澜沧江带新发现的矿床之一。通过野外地质工作和系统构造—|蚀变岩相填图,发现该矿床蚀变类型主要以钾长石化、硅化、绿泥石化、绿帘石化为主,其次为碳酸盐化、绢云母化、黄铁矿化等,且在空间上呈现一定规律,各蚀变带具有明显的叠加现象。依据区内岩石蚀变矿物组合等特点,自断裂带→上盘围岩,共出现4个典型蚀变带,依次为碎裂岩化带→长英岩化—碳酸盐化—绢云母化带→硅化—绿泥石化—绿帘石化带→弱长英岩化安山岩带。矿（化）体主要分布在长英岩化、碳酸盐化、绢云母化带和硅化、绿泥石化、绿帘石化带。由斑岩脉中心至边缘发育钾化带→硅化带→青磐岩化带→绢云母化带,斑岩旁侧围岩中发育放射状石英—方解石—黄铜矿脉。     

黄河万家寨水库右岸岩溶渗漏问题

<正> 万家寨水库位于黄河北干流上游段,坝型为混凝土重力坝,坝高约90米,总库容约9.7亿立方米。从五十年代起直到八十年代初,几个单位对本工程曾相继进行了大量地质勘察工作,并对其作过初步设计。三十年来,各家一致认为万家寨水库不存在永久性岩溶渗漏。本文就此问题谈一点认识,不妥之处,欢迎批评指正。