Marine microbiological studies of mangrove swamps of Killai backwaters

The bacterial populations of mangrove swamps of Killai backwaters (11°21–11°29N, 79°46–79°50E, South India) were studied during August 1968 (Pre monsoon period) and December (post monsoon period). The presence of these groups such as agar digesters, algin digesters, cellulose digesters, sulphate reducers etc., bring about transformation of organic matter in the mangrove swamps. The presence of denitrifiers in mangrove swamps and in association with the molluscs may bring about the precipitation of calcium carbonate by removing the acid radicals such as sulphate and nitrite, increasing alkalinity. The luminiscent bacteria such asVibrio andAeromonas were also isolated in mangrove swamps of Killai backwaters. The iron bacteria likeLeptothrix sp. andGallionella sp. were also isolated from mangrove swamps of Killai backwaters.     

Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

Vertical and lateral transport of organic carbon and the carbon budget in Santa Monica Basin, California

The flux and compositions of solvent-extractable lipid fractions were measured in particulate matter collected periodically by moored sediment traps in the Santa Monica Basin from 1985–1988. The purpose was to assess the compositional changes during settling, the carbon dynamics in the basin and to estimate the impacts of energy-related by-products on the surface sediments. Sediment traps recorded consistently high lipid fluxes in the eastern slope relative to the central basin, reflecting elevated terrigenous carbon inputs possibly from land-based human activities. Generally, lipid fluxes decrease offshore but increase vertically with water depth below 500m, implying lateral transport of particles. The steep decline of flux in the top 500m of the water is related to the rapid decomposition and mineralization of the marine-derived cellular carbon compounds. Less than 5% of the marine lipid components reach the seabed. In contrast, preferential preservation of terrigenous lipid is clearly evident from the chemistry of deeper traps and surface sediments.The lateral transport of particles is reflected in the presence of higher plant-derived lignin phenols and sewage-derived coprostanol and epicoprostanol in the deep trap material as well as in surface sediment throughout the basin. Petroleum triterpanes characteristic of natural seepage also permeates through the entire basin. Based on the data collected from both the trap particulte matter and surface sediments, a carbon budget for the Santa Monica Basin has been constructed.     

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.     

基于LBV变换的遥感影像多步骤分类法研究

面对与日俱增的遥感数据,如何快速准确地进行影像分类成为遥感领域亟待解决的问题。本文在前人的基础上提出了一种基于LBV变换的遥感影像多步骤分类方法。L:地物的总辐射水平,集中反映了裸地的信息;B:地物的可见-红外光辐射平衡,是地面水分状况和水体存在的一个良好指标;V:地物辐射随波段变化的方向和速度向量,能集中反映地面植被状况。多步骤分类法遵循由易到难的原则,能克服类别之间的互相影响,从而提高分类精度。文章结合LBV变换和多步骤分类法的优点对影像进行了分类,结果表明该方法简单易行,且能达到良好的分类精度。     

Cosmic-ray modulation and long-duration solar flare events

The correlation between the long-term intensity variations of cosmic rays at neutron monitor energies and the LDE index measure of solar flares with long-lasting soft X-ray emissions is reported. Three subsequent solar cycles, 20–22, are taken into account and half-monthly data are analyzed. Possible explanation of this correlation is discussed in terms of the recent concepts of cosmic-ray modulation, in particular with merged interaction regions affecting the cosmic-ray intensity.     

Cosmic ray intensity variations and two types of high speed solar streams

This study deals with the short-term variations of cosmic ray intensity during the interval 1973–78. Daily means of high latitude neutron and meson monitors from the same station and those of a low latitude neutron monitor have been analysed using the Chree method of superposed epochs. The zero epoch for the Chree analyses corresponds to the day of a substantial increase (V 200 km s^–1) in the solar wind speed to values of 550 km s^–1 and which persists at such high values for an interval of at least three days. The investigation reveals the existence of two types of cosmic ray intensity variations with distinctly different spectral characteristics. During the interval 1973–76, relative changes in the neutron and meson monitor rates are nearly equal indicating an almost flat rigidity spectrum of variation. During 1977–78, however, the spectrum acquires a negative spectral character similar to that observed for Forbush decreases. We suggest that events of the interval 1973–76 are essentially due to high speed streams associated with solar coronal holes and that events of the interval 1977–78 are due to fast streams from solar active regions with flare activity.     

基于ODBC的地震前兆数据批量卸出与转换程序的实现

介绍了CDBC技术、工作流程及数据传递方式；分析了研制数据卸出与转换程序的关键技术及实现方法；描述了程序的组织结构。通过实例说明了该程序在全国前兆台网中心卸载与转换地震前兆数字化的观测数据中的应用。     

青藏铁路抛石路基的温度特性研究

铁路道渣和片石铺层的对流换热为多孔介质的热传导问题，根据多孔介质中流体热对流的连续性方程、动量方程和能量方程，应用伽辽金法导出了多孔介质对流换热的有限元公式，并对抛石路基和传统道渣路基在未来25a创温度变化进行了预报分析和比较．计算结果表明，在150cm的抛石厚度，片石直径为10cm，年温度较差30℃的倩况下，在路基中心线y＝一5m处，抛石路基下的冻土温度要比传统路基的温度低2．45℃，抛石路基有对其下面的冻土提供冷能的制冷作用，可以保证冻土路基的稳定．因此，推荐该种路基作为青藏铁路高温冻土区的路基结构，可以最大限度地保护冻土区的铁路．     

鄂尔多斯盆地奥陶系海相碳酸盐岩生烃性能与中部长庆气田气源成因研究

用激光 -荧光显微镜等有机岩石学方法 ,挑选样品中有明显生、排烃现象的碳酸盐岩 ,再以测定的这类碳酸盐岩的有机碳含量为依据 ,确定本区高成熟碳酸盐气源岩残余有机碳的下限指标用 0 .13%～ 0 .14 %比较合理。根据碳酸盐岩储层孔隙中充填沥青和皮膜状残余沥青的分布和含量 ,论证了陕参 1井等奥陶系碳酸盐岩地层在地质历史中 ,存在若干油气有效储层。采用连续升温 ,步进取样的热模拟实验方法 ,揭示了本区煤与碳酸盐岩干酪根随热演化作用增强 ,烃类气体产物的干燥系数增大 ,δ1 3C1 、δ1 3C2 明显变重的规律 ,并根据样品实验结果推算 ,本区石炭系—二叠系煤最大生气阶段的δ1 3C1 应为 - 2 7‰～ - 2 8‰ ,碳酸盐岩最大生气阶段的δ1 3C1 应为- 31.7‰～ - 33‰。在气源成因判识中 ,根据主生气期阶段对天然气成藏储聚的贡献最大、甲烷碳同位素较重以及长庆气田风化壳气藏天然气δ1 3C1 重值区并不与石炭系—二叠系煤的镜质组反射率高值区匹配的现象等进行气源判识 ,认为长庆奥陶系风化壳气藏 ,具有下古生界海相碳酸盐岩气与上古生界石炭系—二叠系煤成气的混源成因