Immunological characterization of metallothionein in marine and freshwater fish

Metallothionein (MT) is a low-molecular-weight, cysteine-rich, metalbinding protein found in all vertebrates. The primary structure of the protein is evolutionary conserved, especially the location of cysteine-residues. There are nonetheless significant differences between MTs from different vertebrate classes, notably in major antigenic epitopes. Previous work has shown that MT from different fish species also differ. The objective of the present work was to characterize MT from temperate, sub-tropical and tropical fish species by their relative reactivities with two anti-fish MT antisera. One or both antisera cross-reacted with MT from all fish species investigated. Surprisingly, the relative reactivities of the two antisera with MT from species belonging to different taxonomic groups appeared to reflect differences at the level of family rather than differences between major phylogenetic lineages.     

For your eyes only? Evaluating a coordinated and multiple views tool with a map,a parallel coordinated plot and a table using an eye-tracking approach

Geographic visualization tools with coordinated and multiple views (CMV) typically provide sets of visualization methods. Such configuration gives users the possibility of investigating data in various visual contexts; however, it can be confusing due to the multiplicity of visual components and interactive functions. We addressed this challenge and conducted an empirical study on how a CMV tool, consisting of a map, a parallel coordinate plot (PCP), and a table, is used to acquire information. We combined a task-based approach with eye-tracking and usability metrics since these methods provide comprehensive insights into users’ behaviour. Our empirical study revealed that the freedom to choose visualization components is appreciated by users. The individuals worked with all the available visualization methods and they often used more than one visualization method when executing tasks. Different views were used in different ways by various individuals, but in a similarly effective way. Even PCP, which is claimed to be problematic, was found to be a handy way of exploring data when accompanied by interactive functions.     

Public perceptions of biodiversity in Norway: From recognition to stewardship?

The authors surveyed a representative sample of the Norwegian population (N = 4077) to examine perceptions of biodiversity loss and management, the relative importance of biodiversity loss to other environmental issues, and perceived implications of biodiversity loss. The results showed that 50% of the sample population saw biodiversity as a reality and major environmental issue, and 75% recognized that biodiversity loss occurs. Biodiversity loss was perceived as a lesser global environmental problem than environmental toxins, climate change, air and water pollution, and loss of rainforest, despite the fact that these topics can be difficult to separate since biodiversity loss is a function of other environmental problems. Loss of biodiversity was seen to have negative impacts on people's relationship to the natural environment, to impact environmental resilience, to be at least partly human-induced, and to be an issue of importance and relevance to the general public, not merely to the scientific community. Self-reported levels of knowledge of environmental topics were associated with increasing concern about consequences of reductions in species diversity. The authors conclude that efforts to increase public support for biodiversity conservation can be strengthened by increased emphasis on aesthetic, emotional and cultural aspects of biodiversity.     

Anammox in Tidal Marsh Sediments: The Role of Salinity,Nitrogen Loading,and Marsh Vegetation

Anammox bacteria are widespread in the marine environment, but studies of anammox in marshes and other wetlands are still scarce. In this study, the role of anammox in nitrogen removal from marsh sediments was surveyed in four vegetation types characteristic of New England marshes and in unvegetated tidal creeks. The sites spanned a salinity gradient from 0 to 20 psu. The impact of nitrogen loading on the role of anammox in marsh sediments was studied in a marsh fertilization experiment and in marshes with high nitrogen loading entering through ground water. In all locations, nitrogen removal through anammox was low compared to denitrification, with anammox accounting for less than 3% of the total N₂ production. The highest relative importance of anammox was found in the sediments of freshwater-dominated marshes, where anammox approached 3%, whereas anammox was of lesser importance in saline marsh sediments. Increased nitrogen loading, in the form of nitrate from natural or artificial sources, did not impact the relative importance of anammox, which remained low in all the nitrogen enriched locations (<1%).     

Biomarkers in flounder (Platichthys flesus): an evaluation of their use in pollution monitoring

Flounder (Platichthys flesus) is among the most common fish-species in Norwegian and European estuaries. It lives in or on sediments from which it also finds most of its food. The aim of the present work was to evaluate biomarkers in flounder for possible future use in monitoring programmes. There were clear biomarker responses in flounder following injection of model contaminants benzo[a]pyrene (B[a]P), PCB #156 and Cd, singly or in sequence. Cytochrome P4501A responded following injection of the organic contaminants and metallothionein (MT) following Cd injection. All groups receiving B[a]P, either singly or in combination with other contaminants, accumulated high levels of B[a]P-metabolites in bile. There was little change in glutathione-S-transferase activity (measured using CDNB as substrate) following the treatments. Starvation appeared to affect the response of hepatic MT to Cd, but none of the other biomarkers. PAH in sediments elicited strong biomarker responses in caged flounder, whereas sediment-associated metals appeared to be largely unavailable to flounder in this study.     

Annual fluctuations in sulfur isotope fractionation in the water column of a euxinic marine basin

The sulfur cycle of Mariager Fjord was studied by following the pool of sulfide in the anoxic water and its isotopic composition during a period of 3 yr. Though most of the sulfide accumulating in the fjord was formed in the sediment, the isotopic composition of sulfide in the water was different from the isotopic composition of sulfide diffusing into the water from the sediment. The mean isotopic composition of the water column sulfide (δ³⁴S) varied during the year between −13‰ and −21‰ with the most negative values reached during winter/early spring, while the sulfide diffusing into the water from the sediment had a mean isotope composition of −11.3‰. This annual pattern suggested that processes in the oxidative part of the sulfur cycle were responsible for the excess fractionation, and mass-balance considerations indicated that the excess fractionation of the sulfur isotopes could be accounted for by disproportionation of S⁰ or S₂O₃²⁻ in the water column, but not by water column sulfate reduction or sulfide oxidation alone. MPN counts demonstrated that a population of more than 3 × 10⁴ cells mL⁻¹ capable of growing by disproportionation of these two substrates was present in all depths of the fjord. The results presented in this communication demonstrate that the isotopic depletion of sulfide in anoxic systems may vary between periods of net sulfate reduction versus periods of net sulfide oxidation and indicate that disproportionation of sulfur compounds may be an important step in the sulfur cycle of euxinic basins.     

Vitellogenin in the blood plasma of male cod (Gadus morhua): a sign of oestrogenic endocrine disruption in the open sea?

An ELISA for cod vitellogenin (VTG) has been set up using cod lipovitellin for plate coating and standardisation. The assay has been applied to plasma samples collected from male and female cod caught in three distinct areas around the UK, three areas off the Norwegian coast and also to cod reared initially at an aquaculture site and subsequently maintained at a research station. The aim of the study was to determine whether there were any signs of oestrogenic endocrine disruption in a fish species living offshore. VTG induction was found in male cod caught in the North Sea, the Shetland Box area, in Oslofjord and also in cultivated fish. There was a strong relationship between concentrations of VTG and fish size. There was no evidence that the presence of VTG in the plasma of males is a natural part of their life cycle. On the other hand, the size of fish at which these elevated VTG concentrations appear (ca. 5 kg) is about the size that cod change from feeding primarily on benthic invertebrates to mainly other fish, both benthic and pelagic. The possibility is suggested that large cod pick up oestrogenic endocrine disrupters through the food chain.     

First reports of a mass mortality event across multiple life stages in a mesopelagic jellyfish in high latitude coastal waters

Mass mortality events can occur naturally and may have important ecological impacts on local populations. The abundance and stage structure of the coronate scyphozoan Periphylla periphylla in a Northern Norwegian fjord were studied between October 2010 and August 2011. Developmental stage composition varied for the duration of the study, with early developmental stages only present in the autumn and winter. Records of large number of dead P. periphylla across multiple life stages revealed that a mass mortality event occurred towards the end of the study period. The abundance of live medusa increased from 27 to 39 individuals (ind.)·1,000 m^–3 from October 2010 to February 2011, then fell to <10 ind.·1,000 m^–3 in August 2011. The estimated abundance of dead jellyfish increased from slightly >1 ind.·1,000 m^–3 in October 2010 to >34 ind.·1,000 m^–3 in August 2011, corresponding to an estimated population mortality of >80% on the last sampling date. Mortality increased as the length of high solar irradiance periods increased. Based on previous published evidence that strong light is harmful to P. periphylla, we speculate whether the light climate of this high latitude location may have contributed to the mass mortality of P. periphylla.     

Assessment of lysosomal membrane stability and peroxisome proliferation in the head kidney of Atlantic cod (Gadus morhua) following long-term exposure to produced water components

There is a need for sensitive biological effect methods by which to detect impacts of chronic exposure to low concentrations of contaminants. Two methods shown to be potentially useful for monitoring purposes in fish include lysosomal membrane stability and peroxisome proliferation. These biological endpoints were assessed in Atlantic cod (Gadus morhua) head kidney following exposure to a mixture of produced water components including polycyclic aromatic hydrocarbons, phenol, and alkylphenols. Lysosomal damage of head kidney cells occurred within the first two weeks and did not recover during the entire exposure period (32 weeks). Lysosomal membrane stability was not affected by gender and was responsive at low concentrations of contamination, indicating that lysosomal membrane stability measured in the head kidney could be a useful biomarker for effects of offshore pollution. Peroxisome proliferation, measured as acyl-CoA oxidase activity in the head kidney, appeared to be a potential biomarker in male cod exposed less than 16 weeks.     

Warming permafrost in European mountains

Here we present the first systematic measurements of European mountain permafrost temperatures from a latitudinal transect of six boreholes extending from the Alps, through Scandinavia to Svalbard. Boreholes were drilled in bedrock to depths of at least 100 m between May 1998 and September 2000. Geothermal profiles provide evidence for regional-scale secular warming, since all are nonlinear, with near-surface warm-side temperature deviations from the deeper thermal gradient. Topographic effects lead to variability between Alpine sites. First approximation estimates, based on curvature within the borehole thermal profiles, indicate a maximum ground surface warming of +1 °C in Svalbard, considered to relate to thermal changes in the last 100 years. In addition, a 15-year time series of thermal data from the 58-m-deep Murtèl–Corvatsch permafrost borehole in Switzerland, drilled in creeping frozen ice-rich rock debris, shows an overall warming trend, but with high-amplitude interannual fluctuations that reflect early winter snow cover more strongly than air temperatures. Thus interpretation of the deeper borehole thermal histories must clearly take account of the potential effects of changing snow cover in addition to atmospheric temperatures.