Phylogeography of the pelagic fish Seriola lalandi at different scales: confirmation of inter-ocean population structure and evaluation of southern African genetic diversity

The study investigated the global and regional phylogeography of the yellowtail kingfish Seriola lalandi by examining genetic diversity and population genetic structure of this species at inter-and intra-ocean level and on a regional scale. DNA fragments of two mitochondrial genes, cytochrome b (Cytb) and cytochrome c oxidase subunit I (COI) and one nuclear gene, recombination activating gene 1 (RAG1), were sequenced to investigate the global-scale phylogeography of this species. The population genetic structure within the South Pacific, as well as along the South African coastline, was examined further using six microsatellite markers. Three distinct clades were identified for S. lalandi, which correspond with previously described subspecies of the North-East Pacific, North-West Pacific and the Southern Hemisphere. Within the latter, additional divergence was observed between the South Pacific and the South-East Atlantic regions. Divergence estimates were indicative of a Pacific origin for S. lalandi populations, because of Pleistocene vicariant events. Microsatellite analyses revealed overall significant genetic differentiation between South African and South Pacific samples. This corroborates recent findings on the global phylogeography of the species. No population differentiation was observed within South Africa, indicating high levels of gene flow.     

Small scale turbulent diffusion determined with underwater flow visualization

Dye plumes were generated at three depths in the seasonal thermocline between 7 and 11 m, 22 km south of Key West on 21 August 1980 and photographed at about 10 second intervals with an underwater camera system. Eleven pairs of consecutive pictures are analyzed to determine the mean current vertical shear and the width of the plumes by positioning reference points relative to the rod attached to the camera system. The relative distances of reference points are calibrated with the stereophotogrammetric method for one pair. The eddy diffusivity is calculated by use of a model of turbulent diffusion developed byTaylor (1921). Its values range from 5 to 25 cm²s^–1 for the plume widths ranging from 33 to 132 cm. The Richardson number is calculated for each pair of pictures with the vertical density gradient estimated from temperature profiles. Its values are higher than the critical value of 0.25 except for one case. The diffusivity was higher by orders of magnitude than the molecular one and indicates the presence of turbulence together with billow like features of the plumes in spite of high Richardson numbers. This suggests that the billow turbulence might be caused by effects of surface gravity waves and not by the Kelvin-Helmholtz instability.     

The occultation of κ geminorum by Eros

The predictions and observations of the occultation of κ Gem by Eros on January 24, 1975, are described. At least eight positive and several critical negative observations were made in western New England. The paucity of observations on the western side of the track makes the analysis somewhat ambiguous, but a circular cross section of diameter up to 23 km gives a good fit to most of the available data. Consideration of a crucial, unconfirmed negative observation indicates that an elliptical solution 17 by 7 km (with the long axis in the direction of motion of the “shadow” of Eros) may be preferable, but this does not represent the positive observations so satisfactorily. The ellipse was therefore distorted into a more irregular shape about 20 km long and 7 km wide, rounded on one long side and flattened on the other. Both this irregular shape and the ellipse yield geometric albedos near 0.5, which is considerably higher than has been derived for most other planets or satellites lacking an atmosphere. The albedo that corresponds to the circular solution (0.1) is less than the polarimetric albedo of 0.21 for Eros. It is suggested either that the circle should be warped into an ellipse of dimensions 21 by 13 km, or, if some weight is given to the critical negative observation and the westernmost positive observation, that the profile is a kind of dumbbell.     

Spatial controls on the distribution and dynamics of a marginal snowpack in the Australian Alps

Seasonal snowpacks in marginal snow environments are typically warm and nearly isothermal, exhibiting high inter‐ and intra‐annual variability. Measurements of snow depth and snow water equivalent were made across a small subalpine catchment in the Australian Alps over two snow seasons in order to investigate the extent and implications of snowpack spatial variability in this marginal setting. The distribution and dynamics of the snowpack were found to be influenced by upwind terrain, vegetation, solar radiation, and slope. The role of upwind vegetation was quantified using a novel parameter based on gridded vegetation height. The elevation range of the catchment was relatively modest (185 m), and elevation impacted distribution but not dynamics. Two characteristic features of marginal snowpack behaviour are presented. Firstly, the evolution of the snowpack is described in terms of a relatively unstable accumulation state and a highly stable ablation state, as revealed by temporal variations in the mean and standard deviation of snow water equivalent. Secondly, the validity of partitioning the snow season into distinct accumulation and ablation phases is shown to be compromised in such a setting. Snow at the most marginal locations may undergo complete melt several times during a season and, even where snow cover is more persistent, ablation processes begin to have an effect on the distribution of the snowpack early in the season. Our results are consistent with previous research showing that individual point measurements are unable to fully represent the variability in the snowpack across a catchment, and we show that recognising and addressing this variability are particularly important for studies in marginal snow environments.     

GIS derived synthetic rating curves and HAND model to support on-the-fly flood mapping

Natural Hazards - Acknowledging the devastating consequences of past earthquakes, current research efforts focus on the development of tools for assessing and controlling the risk and losses...     

Zinc and copper levels in Belfast Lough

A survey of dissolved zinc, copper, oxygen and biochemical oxygen demand in Belfast Lough has been carried out. Zinc and to a lesser extent copper occur at elevated levels compared with a non-industrialized area of the Irish Sea. Zinc levels are particularly high in the marginal waters of the north shore of the lough. Dissolved oxygen and biochemical oxygen demand levels indicate that pollution of the lough by oxygendemanding wastes is not a serious problem.     

Genomic resources for the spotted ragged-tooth shark Carcharias taurus

Genomic data can be a useful tool in the management and conservation of biodiversity. Here, we report the development of genomic resources for the spotted ragged-tooth shark Carcharias taurus using genome-wide DNA data from Illumina next-generation sequencing. We explored two commonly used genetic marker types: microsatellites and mitochondrial DNA. A total of 4 394 putative microsatellites were identified, of which 10 were tested on 24 individuals and found to have ideal properties for population genetic analyses. Additionally, we reconstructed the first complete mitochondrial genome of a South African spotted ragged-tooth shark, and highlight the most informative gene regions to facilitate future primer design. The data reported here may serve as a resource for future studies and can ultimately be applied in the sustainable conservation and fisheries management of this apex predator.