病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响

本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2，并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析，旨在了解不同培养基对菌株生长和活性物质合成的影响．结果显示，PT2菌株在不同培养基上的培养特征及生长速率有所不同，液体培养基发酵液抽提物的抗肿瘤活性也有较大差别，其中SYE发酵液抽提物活性最强，对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7．6μg／cm^3、2．4μg／cm^3和3．3μg／cm^3．采用HPLC法对不同抽提物进行分析，发现它们之间的组成成分有显著差异．本文的实验结果表明，不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。     

大冶铁矿国家矿山公园规划实践

大冶铁矿的开发利用在为我国的钢铁事业作出巨大贡献的同时,也给矿区的地表环境、森林资源带来了很大的破坏。矿山公园的规划实施可极大地改善矿山环境,为矿山的可持续发展提供较好的途径。     

Solar-type stars in Cepheus OB 2: Hα emission stars and IRAS point sources

A survey for emission line stars was carried out in 1980 with the Schmidt telescope of Konkoly Observatory in the region of IC 1396 (Kun, 1986a, hereafter referred to as Paper I). This work was aimed at a preliminary mapping of the probable medium mass members of the association Cepheus OB 2. The stars of the region have been followed since then with the same telescope in order to obtain a more complete view on the stellar content of this giant star-forming region. The common variability of the H emission strength makes the sense of repeating such surveys. The new survey resulted in the discovery of 65 further emission stars. Equatorial coordinates and finding charts are given for them.PhotographicBVRI photometry was carried out for all known emission stars of the field. The colourmagnitude and two-colour diagrams derived from theB, V, R, andI magnitudes show that these stars are probably F-G-type (1.5M <M<3M ), partly pre-Main-Sequence members of Cep OB 2, whereas a few of them may be distant red giants.The youngest objects of the region can be found among the IRAS point sources. Their properties are discussed on the basis of IRAS data alone.     

Evolution from Oceanic Subduction to Continental Collision: a Case Study from the Northern Tibetan Plateau Based on Geochemical and Geochronological Data

Two apparently distinct, sub-parallel, paleo-subduction zonescan be recognized along the northern margin of the Tibetan Plateau:the North Qilian Suture Zone (oceanic-type) with ophioliticmélanges and high-pressure eclogites and blueschistsin the north, and the North Qaidam Belt (continental-type) inthe south, an ultrahigh-pressure (UHP) metamorphic terrane comprisingpelitic and granitic gneisses, eclogites and garnet peridotites.Eclogites from both belts have protoliths broadly similar tomid-ocean ridge basalts (MORB) or oceanic island basalts (OIB)in composition with overlapping metamorphic ages (480–440Ma, with weighted mean ages of 464 ± 6 Ma for North Qilianand 457 ± 7 Ma for North Qaidam), determined by zirconU–Pb sensitive high-resolution ion microprobe dating.Coesite-bearing zircon grains in pelitic gneisses from the NorthQaidam UHP Belt yield a peak metamorphic age of 423 ±6 Ma, 40 Myr younger than the age of eclogite formation, anda retrograde age of 403 ± 9 Ma. These data, combinedwith regional relationships, allow us to infer that these twoparallel belts may represent an evolutionary sequence from oceanicsubduction to continental collision, and continental underthrusting,to final exhumation. The Qilian–Qaidam Craton was probablya fragment of the Rodinia supercontinent with a passive marginand extended oceanic lithosphere in the north, which was subductedbeneath the North China Craton to depths >100 km at c. 423Ma and exhumed at c. 403 Ma (zircon rim ages in pelitic gneiss). KEY WORDS: HP and UHP rocks; subduction belts; zircon SHRIMP ages; Northern Tibetan Plateau     

图像数据挖掘软件原型系统的设计与开发

提出了图像数据挖掘软件原型系统的设计与开发的试验框架,阐述了其设计与开发方法,介绍了各个模块的功能、设计与开发情况。     

基于MAPGIS的1∶50万土地利用数据库系统设计与实现

土地利用资料是国家重要的基础地理资料 .针对全国土地利用数据进行建库管理会对国家建设起到重要的作用 .以MAPGIS为基础 ,按照一定的设计原则提出系统总体上应有的数据编辑、查询、统计等六大模块及其主要的功能 .详细说明了在MAPGIS平台的支持下全国土地利用数据库的数据组织方式 ,以及外部属性与图形数据的关联方式、图库中图元的B+ 树索引的结构、数据检索、输出流程等关键技术问题的解决方案 .     

遗传算法在边坡数值计算中的应用

改进了进化方向的遗传算法与有限元数值法，结合并研制了相应的软件。应用该软件对多类型岩土边坡进行弹性模量、内聚力、内摩擦角等参数反演分析，显示误差很小，收敛速度也很快，这说明改进进化方向遗传算法这种新型的优化算法在多类型岩土参数优化估计中具有独特的优势。     

遥测地震仪发射机电路分析及检修

在无线遥测地震仪器中 ,发射机长期使用容易出现输出功率下降、无输出功率、发射频率不准等故障。分析了发射机典型电路——石英晶体主振电路、倍频电路、激励级电路、功率放大级电路的基本原理。排除发射机的故障时 ,先从不同单元电路逐一检查、测试 ,判断故障类型 ,最后进行发射机总调试     

锤击沉管碎石桩处理液化地基的工程实践

结合实际工程,对锤击沉管碎石桩加固处理液化地基的设计、施工控制及处理效果进行了系统论述,对碎石桩设计、施工中遇到的若干问题提出了建议。