X60钢在CO2饱和盐水中缓蚀作用的研究

本文采用动电位极化、线性极化、电偶电流和高压釜挂片失重等法,研究了含氮、硫有机缓蚀剂和/或Me~(2+)对X60钢在CO_2饱和的盐水溶液中的缓蚀作用。结果表明,含氮、硫的有机缓蚀剂在缓蚀过程中,开始是以抑制阳极过程为主,随后是以抑制阴极过程为主的混合型缓蚀剂。如与Me~(2+)复配使用,能明显地提高缓蚀效能。     

大湖塘矿集区燕山成矿阶段矿床成矿系列研究及找矿问题探讨

大湖塘矿集区位于赣西北九岭成矿带西北部位,区内燕山期岩浆岩侵入活动及成矿作用强烈。大湖塘燕山期岩浆岩形成时间分布在130.3 Ma~151.4 Ma之间,可划分为三次侵入:130.3 Ma~134.3 Ma、140.4 Ma~144.4 Ma、146.4 Ma~151.4 Ma;在144.4 Ma~146.4 Ma之间,岩浆活动处于休宁阶段。区内燕山期岩浆岩与矿床在形成时间上十分接近,空间关系上相互依存,并显示特定的分布格局。根据区内矿床的分布特点、产出位置、成矿时间、燕山期花岗岩与矿床的相关性等特征,可将大湖塘矿集区由北向南依次划分为W、Mo—W、Mo、Cu、Pb、Zn—Cu、Pb、Zn、Au、Ag三个成矿系列,并提出了"北钨南铜"的找矿观点。区内双桥山群与成矿,燕山期岩浆岩的起源,矿床等间距分布,以及重力勘探方面的研究应加强。     

新疆塔什库尔干县含稀有金属伟晶岩-碳酸岩的时代——对帕米尔构造结稀有金属成矿作用的启示

帕米尔构造结位于青藏高原北缘,记录了自新元古代晚期以来到新生代完整的特提斯构造演化过程。在长期的构造—岩浆演化过程中,发育了不同时代、不同类型的成矿事件。野外调查表明,在帕米尔北东段,发育了含绿柱石的伟晶岩及含氟碳铈矿的碳酸岩(碳酸岩—伟晶岩组合)。通过独居石及锆石U-Pb定年,识别出该区两期伟晶岩带(包括碳酸岩),分别是新生代和中生代(19～18 Ma和200 Ma),其中早期含绿柱石伟晶岩形成于古特提斯洋关闭后的伸展阶段,而新生代含氟碳铈矿碳酸岩—伟晶岩组合,与新生代陆内走滑伸展事件相关,表明帕米尔构造结伸展走滑启动的时间可能在19 Ma。结合区域地球化学异常特征,表明在帕米尔地区有望在稀有、轻稀土找矿上获得突破。     

我国华东与西南县域主要气象敏感性疾病变化特征及其医疗费用研究

摘要：针对目前医疗费用不断上涨的现实问题,基于安徽省阜南县和贵州省锦屏县相关医疗机构的全部住院数据和气象资料,首先分析两县疾病谱特征,然后从季节和24节气角度,分析两县主要气象敏感性疾病（因天气和气候异常变化而诱发或加重的相关疾病）住院人次和医疗费用的变化特征以及人体舒适度对当地主要气象敏感性疾病发病的影响。结果表明,阜南县和锦屏县人群中住院人数最多的疾病分别是循环和呼吸系统两大类气象敏感性疾病,医疗费用最高的前六位疾病都包含循环、呼吸和消化系统疾病,其三者医疗费用总和分别占两县总医疗费用的44.9%和45.8%,因此,从降低医疗费用的角度来看加强这三类主要气象敏感性疾病的预防十分重要。此外,三种系统疾病医疗费用呈现出明显的季节变化特征：其中,循环系统疾病的医疗费用在季节转换期间明显高于各季节内,呼吸系统疾病冬春季的医疗费用远高于夏秋季节,消化系统疾病夏季医疗费用最高。两县9月的体感温度最接近人体最佳舒适温度,而此时上述三类疾病的住院人次及医疗费用也是相对低点。此研究结果可为当地相关疾病的适时防控、有效降低医疗费用提供科学依据。     

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg²⁺, Fe²⁺, Mn²⁺, Co²⁺ and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L^-1 and 4.33 mg m L^-1 min^-1, respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.     

徐闻南山镇红树林沉积物及红树植物中重金属的累积特征与迁移规律

为探究重金属在红树林沉积物及红树植物中的分布累积及迁移规律,选取了徐闻南山镇红树林为研究对象,通过测定红树林沉积物及红树植物不同部位（根、茎、叶）的重金属质量分数,运用富集因子、生物富集系数、转移系数及相关性分析等方法进行分析。结果表明：1）红树林沉积物重金属质量分数表现为铬（Cr）＞锌（Zn）＞镍（Ni）＞铜（Cu）＞铅（Pb）＞砷（As）＞汞（Hg）＞镉（Cd）,为中等变异程度;除了镍（Ni）元素外,其余7种重金属未超过国家一级标准,除了铅（Pb）元素外,其余7种重金属均超过广东省土壤环境背景值,说明研究区沉积物中重金属具有一定的积累效应。2）沉积物中砷（As）、铜（Cu）、锌（Zn）、汞（Hg）、镍（Ni）、铬（Cr）富集因子值均＞1.5,说明受到轻微人为活动影响;各站位镍（Ni）富集因子值均＞5,结合研究区背景,反映了镍（Ni）受到自然和人为输入的共同影响。3）白骨壤体内重金属主要集中在根部,而红海榄体内重金属在根茎叶中分布相对均匀。白骨壤根茎叶部位的大多数重金属质量分数远高于红海榄,说明白骨壤对重金属的吸附能力比红海榄强。汞（Hg）集中分布在植物的叶片部位,且与其他重金属之间相关性不明显;推测汞（Hg）主要通过叶片吸收进入植物体内,与交通运输污染有关。4）不同红树植物对不同重金属富集能力各异,白骨壤对重金属的富集能力表现为：镉（Cd）＞砷（As）＞铜（Cu）＞锌（Zn）＞汞（Hg）＞铅（Pb）＞镍（Ni）＞铬（Cr）,红海榄表现为：镉（Cd）＞铜（Cu）＞汞（Hg）＞锌（Zn）＞铅（Pb）＞砷（As）＞镍（Ni）＞铬（Cr）。白骨壤和红海榄对汞（Hg）的运移能力都较强;红海榄对镉（Cd）的富集能力和转运能力都较强,而白骨壤对镉（Cd）富集能力较强,转运能力却较弱,这说明红树植物对重金属元素的富集能力与转运能力不存在正比关系。     

韩国“新北方政策”对接“一带一路”倡议：机遇与挑战

韩国“新北方政策”与“一带一路”倡议的对接合作为中韩两国带来了重要发展机遇。文章主要探讨韩国“新北方政策”的内涵及其与“一带一路”倡议对接的进展、机遇与挑战,得出双方对接的合作机遇主要在政策沟通、设施联通、贸易投资和资金融通4个方面,同时也存在着制度性风险、地缘风险和投资合作风险的三大挑战。在具体对接过程中,产业、金融、物流3个领域可能成为突破口。     

Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.