Effects of beta-naphthoflavone on hepatic biotransformation and glutathione biosynthesis in largemouth bass (Micropterus salmoides)

We are investigating the effects of in vivo exposure of prototypical enzyme inducing agents on hepatic biotransformation enzyme expression in largemouth bass (Micropterus salmoides), a predatory game fish found throughout the United States and Canada. The current study targeted those genes involved in biotransformation and oxidative stress that may be regulated by Ah-receptor-dependent pathways. Exposure of bass to beta-naphthoflavone (beta-NF, 66 mg/kg, i.p.) elicited a 7-9-fold increase in hepatic microsomal cytochrome P4501A-dependent ethoxyresorufin O-deethylase (EROD) activities, but did not affect cytosolic GST catalytic activities toward 1-chloro-2,4-dinitrobenzene (CDNB) or 5-androstene-3,17-dione (ADI). Glutathione S-transferase A (GST-A) mRNA expression exhibited a transient, but non-significant increase following exposure to beta-NF, and generally tracked the minimal changes observed in GST-CDNB activities. Expression of the mRNA encoding glutamate-cysteine ligase catalytic subunit (GCLC), the rate-limiting enzyme in glutathione (GSH) biosynthesis, was increased 1.7-fold by beta-NF. Changes in GCLC mRNA expression were paralleled by increases in intracellular GSH. In summary, largemouth bass hepatic CYP1A-dependent and GSH biosynthetic pathways, and to a lesser extent GST, are responsive to exposure to beta-NF.     

Web-cam’s potential for collaborative activities in the Earth sciences

We report here our experiences from using easily acquired web-cam components for collaborative ventures in the Earth sciences. We have used a variety of hardware and different software. We demonstrate from various locations in the U.S.A. the feasibility of using web-cam in multitudinous activities, relevant for conducting research and knowledge dissemination. We summarize the quality of the connections from the various combinations of communicating parties. Today, web-cams can be utilized as an economical and viable means of point-to-point communication in the Earth science community. Greater bandwidth is sorely needed for activities such as multi-party conferencing on the present internet network. We propose that web-cam can be readily deployed as a web-service for facilitating collaborative research over the GRID infrastructure, using the middleware Narada-Brokering. Web-cam will play an important role in the emerging field of geoinformatics.     

Clustering and visualization of earthquake data in a grid environment

We present a web client-server service WEB-IS, which we have developed for remote analysis and visualization of seismic data consisting of both small magnitude events and large earthquakes. We show that the problem-solving environment (PSE) intended for prediction of large magnitude earthquakes can be based on this WEB-IS idea. The clustering schemes, feature generation, feature extraction techniques and rendering algorithms form a computational framework of this environment. On the other hand, easy and fast access both to the seismic data distributed among distant computing resources and to computational and visualization resources can be realized in a GRID framework. We discuss the usefulness of NaradaBrokering (iNtegrated Asynchronous Real-time Adaptive Distributed Architecture) as a middleware, allowing for flexibility and high throughput for remote visualization of geophysical data. The WEB-IS functionality was tested both on synthetic and the actual earthquake catalogs. We consider the application of similar methodology for tsunami alerts.     

Mixed terrigenous—Carbonate sedimentation in the Hispaniola—Caicos turbidite basin

Sedimentation in the 9500 km², 4100 m deep Hispaniòla—Caicos Basin is dominated by turbidity currents. Carbonate turbidites originate from the Bahama Islands, Great Inagua and Caicos at the north end of the basin. Mixed carbonate—non-carbonate flows come from Hispaniola and perhaps Cuba. Most flows originate on insular slopes rather than in shallow water. The relatively low CaCO₃ content of hemipelagic sequences throughout the entire basin reveals that the influence of non-carbonate Hispaniola—Cuba sources is widespread.The basin was sampled with closely spaced piston cores. Sand-layer isopach and frequency maps reveal four or five major basin entry points for turbidity currents. Flow size is proportional to the size of source areas. Average volumes of flows originating from Hispaniola—Cuba, the largest source, are 10⁹ m³. This compares to an average flow volume of 10⁶ m³ for flows derived from the smallest source area, the Southeastern Caicos Bank. Measures of turbidity-current activity, such as thickness and frequency, change in a regular fashion away from each entry point. Average lutite thickness (combining hemipelagic and turbiditic lutite) are greatest near the basin entry points. On the abyssal plain occupying the south half of the basin, Bouma turbidite sedimentary structure sequences tend to be complete. However, on the Caicos Fan, the sedimentary structure sequences in turbidites are characterized by missing or repeated units. Six radiocarbon dates of two widespread presumed pelagic units in the basin yielded younger dates in stratigraphically older positions. The reversed dates are assumed to reflect storm erosion of older sediment on adjacent insular shelves.Consideration of a north—south reflection seismic profile over the basin indicates that the present sediment regimen has pertained through much of the Neogene. The coherence, convergence and termination of reflections in the seismic section are consistent with and tend to confirm conclusions based on the core study regarding the greater extent and volume of sediment deposits derived from the Hispaniola source area.