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Jie DAI Hongzhi TANG Xuegong LI Claire-Lise SANTIN Wenpeng CUI Na LIU Xiaoqing QI Xuehua CUI Alain GROSSI Philippe NOTARESCHI Wei-Jia ZHANG ** Long-Fei WU 《海洋湖沼学报(英文)》2021,(1):297-305
Resazurin(RZ)is a weakly fl uorescent blue dye and can be reduced irreversibly to highly fl uorescent pink resorufi n(RF)that is reduced reversibly to colorless dihydroresorufi n(hRF)by photodeoxygenation,chemical reaction and reductive organic compounds produced through cell metabolism.Because of the reliable and sensitive fl uorescence-color change and noninvasive features,RZ has been used widely as a redox indicator in cell viability/proliferation assays for bacteria,yeast,and mammalian cells.However,RZ is used rarely for physiological characterization of marine microorganisms.Here,we developed a custom-made irradiation and absorption-analysis device to assess the reducing capacity and physiologic status of marine bacterial cultures.We measured the absorption spectra of RZ,RF,and hRF in the presence of the reducing compound Na 2 S and under visible-light irradiation.After establishing appropriate parameters,we monitored the color changes of RZ and its reduced derivatives to evaluate the coherence between reducing capacity,bioluminescence and growth of the deep-sea bacterium Photobacterium phosphoreum strain ANT-2200 under various conditions.Emission of bioluminescence is an oxidation process dependent upon cellular reducing capacity.Growth and bioluminescence of ANT-2200 cell cultures were impeded progressively with increasing concentrations of RZ,which suggested competition for reducing molecules between RZ at high concentration with reductive metabolism.Therefore,caution should be applied upon direct addition of RZ to growth media to monitor redox reactions in cell cultures.Analyses of the instantaneous reduction velocity of RZ in ANT-2200 cell cultures showed a detrimental eff ect of high hydrostatic pressure and high coherence between the reducing capacity and bioluminescence of cultures.These data clearly demonstrate the potential of using RZ to characterize the microbial metabolism and physiology of marine bacteria. 相似文献
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Dai Jie Tang Hongzhi Li Xuegong Santini Claire-Lise Cui Wenpeng Liu Na Qi Xiaoqing Cui Xuehua Grossi Alain Notareschi Philippe Zhang Wei-Jia Wu Long-Fei 《中国海洋湖沼学报》2021,39(1):297-305
Journal of Oceanology and Limnology - Resazurin (RZ) is a weakly fluorescent blue dye and can be reduced irreversibly to highly fluorescent pink resorufin (RF) that is reduced reversibly to... 相似文献
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Qian Xinxin Zhao Yicong Santini Claire-Lise Pan Hongmiao Xiao Tian Chen Haitao Song Tao Li Jinhua Alberto Francois Brustlein Sophie Wu Long-Fei 《中国海洋湖沼学报》2021,39(6):2005-2014
Journal of Oceanology and Limnology - Magnetotactic bacteria (MTB) synthesize intracellular magnetic organelles, magnetosomes, which consist of magnetic crystals that are enveloped in a membrane.... 相似文献
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