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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
2.
PRELIMINARY STUDY ON THE FLOODING AND DROUGHT CLAMITY DURING PAST 1500 YEARS IN THE HAI′AN REGION,JIANGSU PROVINCE 总被引:1,自引:0,他引:1
Collection and arrangement of the historical records of climatic changes and environment evolution,espectial-lyin the aspect of calamities,are made on the history documents of past 1500 years about Haiˊan region,Jiangsu Province.There existed two obvious flooding-drought frequently-occurring periods:one was from 1550 AD to 1850 AD and another was 100 AD to 1200AD.The period of 1550 AD to1850 AD is interrupted by two relatively arid and cold climatic periods:one was from 1630 AD to 1700 AD and another was 1750 AD to 1820 AD.The main characteristic of the calamity periods is that they occurred by turns,and sometimes,both drought and flooding occurred in the same year.The instability of the climatic changes in the Little Ice Age may be the main reason of the frequently-occurring flooding and drought in Haiˊan region.Research results also show that the frequently-occurring periods of flooding and drought is in close relationship with the solar activity,and therefore,occurrence of the flooding and drought may be in relation with the intensity of the solar activity.This hypothesis may need further study in the future. 相似文献
3.
1 INTRODUCTION Blazars, including BL Lac objects, highly polarized and optically violently variable quasars,and flat-spectrum radio quasars (FSRQs), are characterized by highly variable non-thermalemission which dominates their characteristics from radio to y-ray bands. The mechanismbelieved to be responsible for their broadband emission is synchrotron radiation followed by in-verse Compton (IC) scattering at higher energies (e.g. Blandford & Konigl 1979). Relativisticbeaming of a jet… 相似文献
4.
The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin. 相似文献
5.
AN ILLUSTRATIVE STUDY ON LOCAL LANDSCAPEAND ITS LONG-TERM CHANGES BASED ON IKONOS AND HISTORICAL AERIAL PHOTO 总被引:2,自引:0,他引:2
RUANRen-zong ErieCELLISt 《中国地理科学(英文版)》2004,14(2):162-169
Nowadays, the research works on landscape at fine scales using high-resolution images are uncommon.This research is based on the analysis of the combination of remote sensing data (1KONOS imagery acquired in 2002 and historical aerial photo taken in 1942). In the paper, the ecotopes in Qiujiadou and Xishao villages in Yixing City of Jiangsu Province in 1942 and 2002 were compared and landscape changes as well as the causes of the considerable changes were analyzed. It was found that the ecotope changes were at greater level in some aspects such as water surface and perennial vegetation coverage etc. This study at fine scale is globally significant for the rural areas, especially for the subsistence agricultural land, which occupies larger percentage in the earth. And it analyzes the structure of landscape based on a new landscape classification system--stratifications method. 相似文献
6.
Mercury (Hg) was investigated in bone tissues of skua ( Catharacta maccormick) and penguin (Pygoscelis adeliae) collected in the maritime Antarctic using atomic fluorescence spectrometry (AFS) and synchrotron radiation X-ray fluorescence (SR-XRF) method. The total levels of mercury in bone tissues of penguin and skua are much lower than those in other organs (e. g. , kidney, liver). The toxic effects of mercury in bone tissues of seabirds in polar region are not known. We have used SR- XRF method to map the distribution of trace levels of mercury in bones. The levels of mercury are found to be enriched somewhere near the periosteal surface and/or endosteal surface. The distribution of mercury shows strongly correlation with that of some essential elements and probably poses negative effect on the bone metabolism inferring from the relationship of mercury with the other elements. These studies represent a first step toward understanding the toxic effects of mercury on bone of polar animals by suggesting the possible microscopic investigation. 相似文献
7.
A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars. 相似文献
8.
Zhi Xu Cheng Fang Wei-Qun Can Astronomy Department of Nanjing University Nanjing Purple Mountain Observatory Chinese Academy of Sciences Nanjing 《中国天文和天体物理学报》2005,5(5):519-530
Chromospheric lines, including Ha, Lyα, Lyβand CaⅡK, CaⅡ8542, are systemically and quantitatively investigated with respect to the non-thermal excitation and ionization due to particle beam bombardment for a series of solar semi-empirical atmospheric models. As a result we propose to use the contrast in the integrated intensity of hydrogen lines to estimate the total energy flux of the bombarding beam during the solar flare impulsive phase. Partial frequency redistribution is considered in the Lyαline calculation and a smaller intensity enhancement in the Ha line-centers is found than in the previous results of Fang et al. 相似文献
9.
TANGYa XIEJiasui SUNHui 《山地科学学报》2004,1(1):38-45
Dry valleys are a striking geographic landscape in Hengduan Mountains Region and are characterized by low rainfall, desert type of vegetation and fragile environment. Past efforts and resources have been concentrated mainly on rehabilitation of degraded ecosystem and fragile environment, particularly reforestation, while socio-economic development has been largely overlooked. Despite successes in pocket areas, the overall trend of unsustainability and environmental deterioration are continuing. It is important to understand that uplift of the Tibetan Plateau is the root cause of development of dry valleys, and development and formation of dry valleys is a natural process. Human intervention has played a secondary role in development of dry valleys and degradation of dry valleys though human intervention in many cases has speeded up environmental degradation of the dry valleys. It is important to understand that dry valleys are climatic enclaves and an integrated approach that combines rehabilitation of degraded ecosystems and socio-economic development should be adopted if the overall goal of sustainable development of dry valleys is to be achieved. Promotion of niche-based cash crops, rural energy including hydropower, solar energy, biogas and fuelwood plantation is recommended as the priority activities. 相似文献
10.
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk
thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only
the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated
fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs
was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae,
and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs
or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments
containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally
as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it
was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage
of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible
for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of
the starfish had already regionalized before the GV break-down.
Contribution No. 1722 from the Institute of Oceanology, Academia Sinica 相似文献