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The present study investigated conditions for inducing mito-gynogenetic (endomitosis) diploids by hydrostatic pressure in
the large yellow croaker Pseudosciaena crocea. In haploid control groups, the development of eggs was activated with ultraviolet radiated semen. All fry presented typical
haploid syndrome in the haploid control groups, and were verified as haploids using cytometry. After hydrostatic pressure
treatment, morphologically normal fry reappeared at different frequencies according to the intensity and time of pressure
shock. Fry with normal appearance in the pressure treated groups were verified as gynogenetic double haploids (GDHs), containing
only one allele from the female parent at all four diagnostic microsatellite loci. For a fixed duration of 3 min, the optimal
intensity of blocking the first mitosis was determined to be 40 Mpa, which was similar to that of blocking the second meiosis.
There was a “window” of starting time, from 36.1 min to 38.1 min post-insemination at 25.0±1.0°C, within which the production
of GDHs was not significantly different. Maximum production of morphologically normal fries, 9.36%±2.97% of developed eggs,
was found when the eggs were shocked with hydrostatic pressure at 40 Mpa for 3 min, starting from 38.1 min post insemination
at 25.0±1.0°C. 相似文献
2.
The present study investigated conditions for inducing mito-gynogenetic(endomitosis) diploids by hydrostatic pressure in the large yellow croaker Pseudosciaena crocea.In haploid control groups,the development of eggs was activated with ultraviolet radiated semen.All fry presented typical haploid syndrome in the haploid control groups,and were verified as haploids using cytometry.After hydrostatic pressure treatment,morphologically normal fry reappeared at different frequencies according to the intensity and... 相似文献
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