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For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was
injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was
observed that the artificial injection of V. alginolyticus significantly increased the number of erythrocytes, leucocytes, lymphocytes in peripheral blood as well as peripheral serum
antibacterial activity and antibody titer of large yellow croaker, and significantly reduced the number of peripheral blood
granulocytes as compared with those in the control group. No significant difference in acid phosphytase and superoxide dismutase
activity of serum was detected between the two groups. It is suggested that non-specific immune factors including leucocytes
and anti-bacteria substance in peripheral blood played important role at the initial stage of infection, and specific immune
factors such as antibody then played important role in response to anti-infection at the latter stage.
Supported by the National High Technology Research and Development Program of China (863 program, No. 2007AA09Z115) and Technology
Program of Xiamen (No. 3502Z73019) 相似文献
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