GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth.     

GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

ＩＮＴＲＯＤＵＣＴＩＯＮＧｒｏｗｔｈｈｏｒｍｏｎｅ (ＧＨ)ｉｓａｐｏｌｙｐｅｐｔｉｄｅｐｒｏｄｕｃｅｄｉｎｔｈｅｐｉｔｕｉｔａｒｙｇｌａｎｄａｎｄｈａｓｂｅｅｎｓｈｏｗｎｔｏｐｌａｙａｎｅｓｓｅｎｔｉａｌｒｏｌｅｉｎｔｈｅｓｔｉｍｕｌａｔｉｏｎｏｆｓｏｍａｔｉｃｇｒｏｗｔｈａｎｄｄｅｖｅｌｏｐｍｅｎｔｉｎｖｅｒｔｅｂｒａｔｅ .Ｓｅｖｅｒａｌｓｔｕｄｉｅｓｗｅｒｅｃｏｎｄｕｃｔｅｄｏｎｔｈｅｇｒｏｗｔｈ ｐｒｏｍｏｔｉｎｇａｃｔｉｖａｔｉｏｎｏｆｖａｒｉｏｕｓｔｅｌｅｏｓｔＧＨｓｉｎｓａｌｍｏｎｉｄ…