Variations in the Rate of Anaerobic Succinate Accumulation within the Central and Marginal Regions of an Euryoxic Bivalve Mantle

Abstract. Anaerobic metabolism in the central and marginal portions of the mantle of Mercenaria mercenaria was compared. Anaerobic succinate accumulation was more rapid in the central region. This difference may be due to higher phosphoenolpyruvate carboxykinase activity in the central region. Thus, the central region is more specialized for anaerobic metabolism and the marginal region more for net shell growth. The original rate of succinate accumulation in the mantle is similar in isolated mantles and intact clams, suggesting that mantle succinate production does not require translocation of precursors from other tissues. However, in intact clams, the rate of succinate accumulation in the central region of the mantle slows after four hours. The reduced rate is probably caused by reducing the metabolic rate. Succinate accumulation and shell dissolution are slower in freshly collected clams than in clams that had been stored anaerobically. The difference may be due to induction of PEPCK synthesis during storage. Shell derived calcium did not accumulate in the mantle and, therefore did not alter the intracellular calcium concentration in the mantle.     

The Ah receptor in marine animals: phylogenetic distribution and relationship to cytochrome P4501A inducibility

In mammals, the induction of cytochrome P4501A forms by chlorinated dibenzo-p-dioxins, chlorinated dibenzofurans, and halogenated biphenyls is under control of a soluble protein known as the Ah (aromatic hydrocarbon) receptor. Little is known about the presence and properties of the Ah receptor in other vertebrate and invertebrate species. In these studies, we sought evidence for an Ah receptor in the liver or liver-equivalent of 20 species of marine and freshwater animals, using the photoaffinity ligand 2-azido-3-[¹²⁵I] iodo-7,8-dibromodibenzo-p-dioxin (N₃[¹²⁵I]Br₂DD). Specific labeling of cytosolic proteins by N₃[¹²⁵I]Br₂DD was observed in seven species of teleost and elasmobranch fish, in PLHC-I fish hepatoma cells, and in beluga whales. No specifically labeled proteins were found in cytosol from two species of agnathan fish nor in any of nine invertebrate species representing eight classes of four phyla. The presence or absence of specifically labeled polypeptides corresponds with the inducibility of cytochrome P4501A and sensitivity to the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related planar halogenated aromatic hydrocarbons in many of these groups. Thus, Ah receptor function may have arisen early invertebrate evolution and has been conserved from elasmobranch and teleost fish to mammals.     

Changes in a rocky shore community: An evaluation of monitoring

The basic aim of this study was to record the changes in the communities on a moderately exposed rocky shore by a programme of repeated non-destructive sampling (‘monitoring’) and to determine the causes of these changes. Possible causative factors were investigated by the combined approach of analysing detailed sea-temperature and meteorological data for the period of the study and establishing manipulative field experiments.Fixed quadrats (2 m by 1 m) at three levels were visited at 6–8 weekly intervals for 30 months and the abundance and spatial pattern of major species assessed. In the ‘High’ quadrat the balance between Fucus spiralis and F. vesiculosus changed, and an outburst of ephemeral algae occurred following a decline in overall canopy cover. In the ‘Mid’ quadrat F. vesiculosus decreased considerably from its initial cover and virtually disappeared by the end of the study. In the ‘Low’ quadrat F. vesiculosus increased from being absent to comprising 20% of the canopy, whilst Semmibalanus (= Balanus) balanoides and ‘understorey’ algae decreased in cover. Actinia equina, Nucella lapillus and Patella vulgata showed marked changes in number at all levels where they occurred.Only certain of these changes could be attributed to the physical environment—most were biologically controlled. For example, manipulative experiments showed that the blooms of ephemeral algae and the decline of Patella in the high-shore quadrat, and the decline of Actinia in the mid-shore quadrat, were due to decreases in the fucoid canopy. These canopy changes were, in turn, the result of biological cycles which are characteristic of these communities. However, even with the support of manipulative experiments, it was not possible to account for (or predict) all of the observed natural changes. The extent of these fluctuations poses a serious obstacle to the recognition of any but very gross pollution-induced effects. The conclusion must be that the monitoring of rocky shores as a means of detecting and measuring pollution is likely to be an unproductive investment of time and resources.     

The effects of sewage discharge on water quality and phytoplankton of Hawai'ian coastal waters

The effects of sewage discharge on algal populations and the quality of Hawai'ian coastal waters were investigated. Two outfalls were studied. One discharges primary treated sewage and the other discharges secondary treated sewage but are otherwise similar. This enabled comparisons of the effects of these different levels of treatment on the water quality and algal productivity of receiving waters. Plumes were followed and repeatedly sampled in a time-series manner. Rhodamine dye was used as a conservative tracer to compare the dilution behavior of the plume constituents MRP, NO(3)+NO(2), NH(4), Silicate, TDP, TDN, total bacteria, PC, and PN. Rates of initial dilution ranged from two to almost three orders of magnitude, and were in reasonable agreement with engineering model predictions. Dilution of plume constituents approximated that of Rhodamine until background concentrations were reached, typically within 10 min of discharge. Chl a concentrations did not increase through time in the primary sewage plume but did increase up to 30% in the secondary sewage plume. However, rates of far-field dilution were so rapid that the increase could not have been due to algal growth. The increase was attributed to the plume mixing with a water mass whose relative chl a concentrations were greater. Rates of secondary dilution ranged from 2 to 3 orders of magnitude resulting in total dilutions of 10(5)-10(6) within 3 h of discharge. These rates of secondary dilution were much greater than model predictions. From a nutrient standpoint, secondary treatment exhibited no advantages over primary treatment because dilutions were so rapid.     

Beyond world cities: Central America in a global space of flows

Central America is a region without a world city. Traditionally, the region's national projects have been based upon openness to the world economy: how do the region's contemporary transnational projects connect to the world economy under the new conditions of openness that is contemporary globalization? Focusing upon advanced producer services, three connections into the world city network are identified: global service firms operating directly in Central American cities, local service firms operating indirectly through extra-regional world cities and global service firms operating through Latin American regional offices. Miami is confirmed as the primary world city through which Central America connects into the world economy     

Precambrian–Cambrian boundary interval deposition and the marginal platform of the Avalon microcontinent

Thick terminal Proterozoic–lowest Cambrian successions allow reference of the Saint John, New Brunswick, and MacCodrum Brook, southern Cape Breton Island, areas to the marginal platform of the Avalon microcontinent. Marginal-platform siliciclastic-dominated sequences form a cover on Late Precambrian arc successions from southern New Brunswick to North Wales. Their deposition in fault-bounded basins began with the origin of the Avalon microcontinent and development of a persistent transtensional regime in the latest Precambrian. The terminal Proterozoic–lowest Cambrian on the Avalonian marginal platform consists of three successive lithofacies associations: lower subaerial rift to marginal-marine facies; overlying cool-water, wave-influenced, marine platform sandstones and shales; and higher macrotidal quartz arenites (=Avalonian depositional sequences 1–2). Only the Lower Cambrian macrotidal quartz arenites onlap southeast, where they form the oldest Cambrian unit on the inner platform. These major lithofacies are the Rencontre, Chapel Island, and Random formations, respectively, in Avalonian North America. Southwest thinning of the Rencontre–Chapel Island–Random interval in southern New Brunswick reflects slower subsidence of a fault-bounded area in the city of Saint John. The depositional sequence 1–2 unconformity, which falls in the sub-trilobitic Lower Cambrian Watsonella crosbyi Zone of the Chapel Island Formation, persists for 650 km along the marginal platform from southeastern Newfoundland to southern New Brunswick and, potentially, appears in Cape Breton Island. Latest Precambrian-earliest Cambrian epeirogenic and depositional history was very uniform along the marginal platform, and a unified lithostratigraphic nomenclature is appropriate.     

Solitary wave generation dynamics at Luzon Strait

A high resolution modeling study is undertaken, with a 2.5-dimensional nonhydrostatic model, of the generation of internal waves induced by tidal motion over the ridges in Luzon Strait. The model is forced by the barotropic tidal components K1, M2, and O1. These tidal components, along with the initial density field, were extracted from data and models. As the barotropic tide moves over the Luzon Strait sills, there is a conversion of barotropic tidal energy into baroclinic tidal energy. Depressions are generated that propagate towards the Asian Seas International Acoustics Experiment (ASIAEX) test site on the Chinese continental shelf. Nonlinear effects steepen the depressions, frequency and amplitude dispersion set in, and disintegration into large amplitude solitary waves occurs. The effects of varying the initial density field, tidal component magnitudes, as well as adding a steady background current to represent the occasional excursions of the Kuroshio Current into the strait, are considered.Depressions are generated at each of the two sills in Luzon Strait which radiate away, steepening and evolving into internal solitary wave trains. Baroclinic fluxes of available potential energy, kinetic energy and linear are calculated for various parameter combinations. The solitary wave trains produced in the simulations generally consist of large amplitude wave trains alternating with small amplitude wave trains. During strong tidal flow, Kelvin–Helmholtz type instabilities can develop over the taller double-humped sill. The solitary waves propagating towards the ASIAEX test site have been observed to reach amplitudes of 120–250 m, depending on the tidal strength. ASIAEX observations indicate amplitudes up to 150 m and the Windy Island Experiment (WISE) measurements contain magnitudes over 200 m. The model results yield solitary wave amplitudes of 70–300 m and half widths of 0.60–3.25 km, depending on parameter values. These are in the range of observations. Measurements by Klymak et al. (2006), in the South China Sea, exhibit amplitudes of 170 m, half widths of 3 km and phase speeds of 2.9 m s^?1. Model predictions indicate that the solitary waves making up the wave packet each experience different background currents with strong near surface shear.The energy in the leading soliton of the large amplitude wave trains ranges between 1.8 and 9.0 GJ m^?1. The smaller value, produced using barotropic tidal currents based on the Oregon State University data base, is the same as the energy estimated to be in a solitary wave observed by Klymak et al. (2006). Estimates of the conversion of barotropic tidal energy into radiating internal wave energy yield conversion rates ranging between 3.6% and 8.3%.     

Crustal observations beneath the southern North Sea and their tectonic and geological implications

In 1991, a deep seismic reflection line, MPNI-9101, was acquired in the southern North Sea from the Mesozoic Broad Fourteens Basin, across the West Netherlands Basin onto the London-Brabant Massif (LBM). The resultant section shows a strongly reflective lower crust beneath the area of Mesozoic basin development. This lower crustal reflectivity continues to be strong beneath the LBM. The travel time to the base of the reflective zone increases from approximately 11.0 s beneath the Mesozoic basins to 12.5 s beneath the LBM, suggesting a southward thickening of the crust (Rijkers et al., 1993). Based on these travel times and information from deep wells and refraction surveys. Moho depth is estimated to increase from about 31 km beneath the Mesozoic basins to about 38 km beneath the LBM. This difference in depth to the Moho can partly be explained by coaxial stretching of the crust beneath the Mesozoic basins. In comparison with the Mesozoic basins, the crust beneath the LBM was thickened during the Caledonian and Variscan orogenies.     

Robust kriging—A proposal

Geological data frequently have a heavy-tailed normal-in-the-middle distribution, which gives rise to grade distributions that appear to be normal except for the occurrence of a few outliers. This same situation also applies to log-transformed data to which lognormal kriging is to be applied. For such data, linear kriging is nonrobust in that (1)kriged estimates tend to infinity as the outliers do, and (2)it is also not minimum mean squared error. The more general nonlinear method of disjunctive kriging is even more nonrobust, computationally more laborious, and in the end need not produce better practical answers. We propose a robust kriging method for such nearly normal data based on linear kriging of an editing of the data. It is little more laborious than conventional linear kriging and, used in conjunction with a robust estimator of the variogram, provides good protection against the effects of data outliers. The method is also applicable to time series analysis.