Photoadaptation of carboxylating enzymes and photosynthesis during a spring bloom

Properties of the light saturation curve of photosynthesis and ribulose-1,5-bisphosphate carboxylase (RuBPC) activity are shown to change qualitatively in a natural population of marine phytoplankton during a spring bloom. Evidence is presented to show that these changes constitute photoadapative responses to increasing irradiance. As irradiance increased during the bloom, both the level of light-saturated photosynthesis (P_m) and the initial slope of the light saturation curve (α = photosynthetic efficiency) increased whether those parameters were normalized to chlorophyll a concentration (P_m^b, α^b) or to cell numbers (P_m^c, α^c). The magnitudes of these changes were such that I_k (= P_m/α, the photoadaptation parameter) did not change, but I_m, the light intensity at which photosynthesis becomes saturated, increased. RuBPC activity, both chlorophyll a (RuBPC^b) and cell number normalized (RuBPC^c), also increased during the bloom. We suggest that these adaptations were achieved by simultaneously increasing the number of photosynthetic units, proportionately decreasing the photosynthetic unit size, and increasing both the concentrations of the enzymes of the dark reactions and possibly also of photosynthetic electron transport components.We also observed diminished levels of photoinhibition in the high light adapted cells late in the bloom and have suggested that this was a consequence of the same suite of physiological changes.In situ carbon fixation per cell increased during the bloom whereas no change occurred in this parameter when normalized to chlorophyll a concentration. Although these photoadaptive responses thus permitted carbon to be fixed in situ more rapidly per cell, at a constant efficiency with respect to investment of energy in the photosynthetic apparatus, they did not result in a change in growth rate. Based on consideratios of the role of time scale in physiological adaptation, however, it is suggested that the observed alterations in photosynthesis with increasing irradiance might permit a cell to more rapidly fill an energy quota for division, possibly an advantage in a mixing environment in which energy is patchily distributed, both spatially and temporalyy.Phosphoenolpyruvate carboxylase activity when normalized to chlorophyll a (PEPC^b) did not change during the bloom while chlorophyll a normalized dark carbon fixation decreased sharply and was quantitatively small compared to PEPC^b. On this basis and considering that RuBPC^b increased during the bloom, it is suggested that, although PEPC may be involved in dark carbon fixation, its most important quantitative role is probably an indirect one in light dependent photosynthesis.We have also considered the relevance of laboratory results on photoadaptation to interpretations of field studies and have suggested that batch culture studies must be treated with caution but that turbidistat and semi-continuous methods provide reasonable simulations of natural conditions.     

ISO Far-IR spectroscopy of IR-bright galaxies and ULIRGs

Based on far-infrared spectroscopy of a small sample of nearbyinfrared-bright and ultraluminous infrared galaxies (ULIRGs) with theISO Long Wavelength Spectrometer we find adramatic progression in ionic/atomic fine-structure emission line andmolecular/atomic absorption line characteristics in these galaxiesextending from strong [O III]52,88 m and [N III]57 m lineemission to detection of only faint [C II]158 m line emissionfrom gas in photodissociation regions in the ULIRGs. The molecularabsorption spectra show varying excitation as well, extending fromgalaxies in which the molecular population mainly occupies the groundstate to galaxies in which there is significant population in higherlevels. In the case of the prototypical ULIRG, the merger galaxy Arp220, the spectrum is dominated by absorption lines of OH, H₂O, CH,and [O I]. Low [O III]88 m line flux relative to the integratedfar-infrared flux correlates with low excitation and does not appear tobe due to far-infrared extinction or to density effects. A progressiontoward soft radiation fields or very dusty H II regions may explainthese effects.     

Structure of a Large low-Latitude Coronal Hole

Coronal holes on the Sun are the source of high-speed solar wind streams that produce magnetic disturbances at the Earth. A series of multi-wavelength, multi-instrument observations obtained during the 1996 Whole Sun Month campaign examined a large coronal hole in greater detail than ever before. It appeared on the Sun in August, and extended from the north pole to a large active region in the southern hemisphere. Its physical and magnetic structure and subsequent evolution are described.     

The Henry's Law Constants of the Haloacetic Acids

Henry's law constants K_H (mol kg^-1 atm^-1) have been measured between 278.15 K and 308.15 K for the following organic acids: CH₂FCOOH (ln(K_H[298.15 K]) = 11.3 ± 0.2), CH₂ClCOOH (11.59 ± 0.14), CH₂BrCOOH (11.94 ± 0.21), CHF₂COOH (10.32 ± 0.10), CHCl₂COOH (11.69 ± 0.11), CHBr₂COOH (12.33 ± 0.29), CBr₃COOH (12.61 ± 0.21), and CClF₂COOH (10.11 ± 0.12). The variation of K_H with temperature was determined for all acids except CH₂FCOOH and CBr₃COOH, with _r H° for the dissolution reaction ranging from –85.2 ± 2.6 to –57.1 ± 2.5 kJ mol^-1, meaning that their solubility is generally more sensitive to temperature than is the case for the simple carboxylic acids. The Henry's law constants show consistent trends with halogen substitution and, together with their high solubility compared to the parent (acetic) acid (ln(K_H[298.15 K]) = 8.61), present a severe test of current predictive models based upon molecular structure. The solubility of haloacetic acids and strong dissociation at normal pH mean that they will partition almost entirely into cloud and fog in the atmosphere (0.05–1.0 g H₂O m^-3), but can reside in both phases for the liquid water contents typical of aerosols (10^-5-10^-4 g H₂O m^-3).     

An assessment of chlorophyll-a concentration spatio-temporal variation using Landsat satellite data,in a small tropical reservoir

Traditional approaches to monitoring aquatic systems are often limited by the need for data collection which often is time-consuming, expensive and non-continuous. The aim of the study was to map the spatio-temporal chlorophyll-a concentration changes in Malilangwe Reservoir, Zimbabwe as an indicator of phytoplankton biomass and trophic state when the reservoir was full (year 2000) and at its lowest capacity (year 2011), using readily available Landsat multispectral images. Medium-spatial resolution (30 m) Landsat multispectral Thematic Mapper TM 5 and ETM+ images for May to December 1999–2000 and 2010–2011 were used to derive chlorophyll-a concentrations. In situ measured chlorophyll-a and total suspended solids (TSS) concentrations for 2011 were employed to validate the Landsat chlorophyll-a and TSS estimates. The study results indicate that Landsat-derived chlorophyll-a and TSS estimates were comparable with field measurements. There was a considerable wet vs. dry season differences in total chlorophyll-a concentration, Secchi disc depth, TSS and turbidity within the reservoir. Using Permutational multivariate analyses of variance (PERMANOVA) analysis, there were significant differences (p < 0.0001) for chlorophyll-a concentration among sites, months and years whereas TSS was significant during the study months (p < 0.05). A strong positive significant correlation among both predicted TSS vs. chlorophyll-a and measured vs. predicted chlorophyll-a and TSS concentrations as well as an inverse relationship between reservoir chlorophyll-a concentrations and water level were found (p < 0.001 in all cases). In conclusion, total chlorophyll-a concentration in Malilangwe Reservoir was successfully derived from Landsat remote sensing data suggesting that the Landsat sensor is suitable for real-time monitoring over relatively short timescales and for small reservoirs. Satellite data can allow for surveying of chlorophyll-a concentration in aquatic ecosystems, thus, providing invaluable data in data scarce (limited on site ground measurements) environments.     

Solubility of HOCl in water and aqueous H2SO4 to stratospheric temperatures

Henry's law constants K_H (mol kg^–1 atm^–1) for the reaction HOCl_(g)=HOCl_(aq) near room temperature, literature data for the associated enthalpy change, and solubilities of HOCl in aqueous H₂SO₄ (46 to 60 wt%) at temperatures relevant to the stratosphere (200 KT230 K) are shown to be thermodynamically consistent. Effective Henry's law constants [H*=mHOCl/pHOCl, in mol kg^–1 atm^–1] of HOCl in aqueous H₂SO₄ are given by: ln(H*)=6.4946–mH₂SO₄(–0.04107+54.56/T)–5862 (1/T_o–1/T) where T(K) is temperature and T_o=298.15K. The activity coefficient of HOCl in aqueous H₂SO₄ has a simple Setchenow-type dependence upon H₂SO₄ molality.     

Real-time measurement of volcanic SO2 emissions: validation of a new UV correlation spectrometer (FLYSPEC)

A miniaturized, lightweight and low-cost UV correlation spectrometer, the FLYSPEC, has been developed as an alternative for the COSPEC, which has long been the mainstay for monitoring volcanic sulfur dioxide fluxes. Field experiments have been conducted with the FLYSPEC at diverse volcanic systems, including Masaya (Nicaragua), Poás (Costa Rica), Stromboli, Etna and Vulcano (Italy), Villarica (Chile) and Kilauea (USA). We present here those validation measurements that were made simultaneously with COSPEC at Kilauea between March 2002 and February 2003. These experiments, with source emission rates that ranged from 95 to 1,560 t d⁻¹, showed statistically identical results from both instruments. SO₂ path-concentrations ranged from 0 to >1,000 ppm-m with average correlation coefficients greater than r ²=0.946. The small size and low cost create the opportunity for FLYSPEC to be used in novel deployment modes that have the potential to revolutionize the manner in which volcanic and industrial monitoring is performed.     

Effect of artificial maturation on carbazole distributions, as revealed by the hydrous pyrolysis of an organic-sulphur-rich source rock (Ghareb Formation, Jordan)

Hydrous pyrolysis experiments were performed on the Ghareb Formation (Upper Cretaceous, Jordan), a carbonate- and organic-rich (TOC 19.6%) source rock, using a temperature range of 200 to 360°C (72 h). The original sediment contains only low amounts of carbazoles, (maximum 2.2 μg/g bitumen for 1-methylcarbazole). With increasing thermal maturation, intense generation begins at temperatures only in excess of 300°C, reaching a maximum at 360°C. Likewise, during natural maturation, generation occurs at later stages of maturity (e.g. for Tithonian source rocks at >0.81% R_r and for Posidonia Shale at >0.88% R_r). Some isomeric changes during hydrous pyrolysis do not resemble those in nature whereas others do. The relative abundances of selected C₁- and C₂-alkylcarbazoles on ternary diagrams reveal differences, whereas the benzo[a]carbazole/benzo[a]carbazole+benzo[c]carbazole ratio is closely similar. The latter result supports the contention that maturation plays a key role in controlling carbazole distributions in source rocks. However, the results for alkylcarbazoles, especially the C₂-carbazoles, are not easy to interpret.