An experimental study of magnesium-isotope fractionation in chlorophyll-a photosynthesis

Measurements are presented of the magnesium isotopic composition of chlorophyll-a, extracted from cyanobacteria, relative to the isotopic composition of the culture medium in which the cyanobacteria were grown. Yields of 50-93% chlorophyll-a were achieved from the pigment extracts of Synechococcus elongatus, a unicellular cyanobacteria. This material was then digested using concentrated nitric acid to extract magnesium. Separation was accomplished using columns of cation-exchange resin, which achieved a 103 ± 10% yield of magnesium from chlorophyll-a. This procedure ensured accurate measurement of the magnesium-isotopic ratios without isobaric interferences using a multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). We find a slight depletion in the heavier isotopes of magnesium in chlorophyll-a relative to culture medium, early growth phase: Δ²⁶Mg = −0.71(±0.35)‰ and Δ²⁵Mg = −0.37(±0.18)‰; late growth phase: Δ²⁶Mg = −0.53(±0.20)‰ and Δ²⁵Mg = −0.26(±0.11)‰, due to an apparent mass-dependent fractionation. We suggest that the small fractionation results from chelation during intracellular processes. A likely candidate for this chelation step involves the magnesium-chelatase enzyme, which mediates the insertion of magnesium to the tetrapyrrole ring during chlorophyll-a biosynthesis. Proof of this hypothesis can be tested with biological controls whereby steps in the enzymatic pathways of chlorophyll synthesis are selectively suppressed.     

Validation of a fish farm waste resuspension model by use of a particulate tracer discharged from a point source in a coastal environment

To validate a resuspension model of particulate material (salmonid farm wastes), a UV fluorescent particle tracer was selected with similar settling characteristics. Tracer was introduced to the seabed (water depth ≈30 m) and sediment samples taken on days 0, 3, 10, 17 and 30 to measure the horizontal and vertical distribution of tracer in sediments. A concentric sampling grid was established at radii of 25, 50, 100, 150, 200, 400, 700 and 1, 000 m from the source on transects 30° apart. The bulk of the deployed tracer was initially concentrated in an area 25 m radius from the release point; tracer was observed to steadily decrease to zero over a period of 30 days. In a 200 m region measured from the release point in the direction of the residual current, the redeposition of tracer was low. A Lagrangian particle tracking model was validated using these observed data by varying resuspension model parameters within limits to obtain the best agreement between spatial and temporal distributions. The validated model generally gave good predictions of total mass budgets (±7% of total tracer released), particulary where tracer concentrations were high near the release point. Best fit model parameters (critical erosion shear stress=0.018 N m⁻², erodibility constan=60 g m⁻² d⁻¹) are at the low end of reported parameters for coastal resuspension models. Such a low critical erosion shear stress indicates that the frequency of resuspension and deposition events for freshly deposited material is high.     

Geochemistry and geochronology of proterozoic tholeiite dykes of east antarctica: evidence for mantle metasomatism

Two episodes of tholeiite dyke emplacement have been identified in Archaean high-grade metamorphics of the Napier Complex in Enderby Land. Middle Proterozoic Amundsen dykes are typical continental tholeiites and most of the chemical variation in individual suites can be explained in terms of different degrees of partial melting and low-pressure crystal fractionation. Group I Amundsen tholeiites were derived from a relatively homogeneous source region 1,190±200 m.y. ago, whereas that of the group II Amundsen tholeiites was chemically and isotopically heterogeneous. Group II dykes have various degrees of enrichment in incompatible elements, and commonly show normalised trace element abundance patterns with negative Nb anomalies. These features imply variable metasomatism of the source region by a volatile-rich fluid phase (rather than a melt of any observed igneous composition) enriched in K, Rb, Ba, Th, and possibly La and Ce.Early Proterozoic (2,350±48 m.y.) tholeiites were emplaced at considerable depths in the crust during the waning stages of granulite-facies metamorphism and include a high-Mg suite of possible komatiitic affinity, ranging in composition from hypersthene-rich tholeiite (norite) to quartz-rich tholeiite. They tend to have higher ratios of highly to moderately incompatible elements (e.g., K/Zr, K/Ce), and larger Nb anomalies (i.e., higher K/Nb) compared with middle Proterozoic tholeiites, suggesting derivation from more enriched source regions. Isotopic data are not compatible with significant crustal contamination, but constrain source metasomatism to a time immediately before emplacement. Metasomatism of the source region of the much younger group I tholeiites may have been contemporaneous with that of the high-Mg suite.     

The Harry Creek Deformed Zone,a retrograde schist zone of the Arunta Block,central Australia

The Harry Creek Deformed Zone, a retrograde schist zone of epidote amphibolite facies grade, which separates the granulite facies Utralanama Block from the amphibolite facies Ankala Block in the southeastern Strangways Range, N.T., is typical of the retrograde schist zones transecting the Arunta Block. Associated with the deformed zone is a small deformed granitic pluton and its various offshoots—the Gumtree Granite Suite—which provides structural and geochrono‐logical evidence that the Harry Creek Deformed Zone has had a polyphase deforma‐tional history. Early movements within the deformed zone pre‐dated intrusion of the Gumtree Granite Suite and resulted in the movement of the Utralanama and Ankala Blocks into their present juxtaposition. Reactivation of much of the zone during the Alice Springs Orogeny brought about the schistose character of the zone and the deformation of the granitic rocks. Further minor reactivation of the zone, subsequent to the main phase of the Alice Springs Orogeny, resulted in limited development of pseudotachylytes.

The age of the granite (990 ± 13 m.y.) gives a minimum age for initiation of the zone, and evidence for the nature of the structures associated with the early movements is presented. It is suggested that the Harry Creek Deformed Zone represents a post‐orogenic wrench fault which has been reactivated. Early movements, which were of a brittle transcurrent nature, brought about major uplift (up to 10 km) to the north, and lateral movements may have been of the order of 60 km in a sinistral sense. Comparison with the Redbank Zone indicates many similarities, suggestive of a similar history.     

Stability of outer planetary orbits around binary stars: A comparison of Hill's and Laplace's stability criteria

A comparison is made between the stability criteria of Hill and that of Laplace to determine the stability of outer planetary orbits encircling binary stars. The restricted, analytically determined results of Hill's method by Szebehely and co-workers and the general, numerically integrated results of Laplace's method by Graziani and Black are compared for varying values of the mass parameter =m ₂/(m ₁+m ₂). For 00.15, the closest orbit (lower limit of radius) an outer planet in a binary system can have and still remain stable is determined by Hill's stability criterion. For >0.15, the critical radius is determined by Laplace's stability criterion. It appears that the Graziani-Black stability criterion describes the critical orbit within a few percent for all values of .     

SmNd isotopic systematics of Enderby Land granulites and evidence for the redistribution of Sm and Nd during metamorphism

Measurements of¹⁴³Nd/¹⁴⁴Nd and¹⁴⁷Sm/¹⁴⁴Nd are reported for whole rocks and mineral separates from granulites of the Napier Complex at Fyfe Hills. Charnockites, leuconorites and gabbros yield a whole rock SmNd isochron age of3060 ± 160m.y. and an initial¹⁴³Nd/¹⁴⁴Nd ratio of0.50776 ± 10 (?_Nd(3060m.y.) = ?2.0 ± 1.8). The negative ?_Nd value and the presence of geologically induced dispersion in the data suggest that the isochron age does not represent the time of primary crystallization of the complex but instead indicates a time of later redistribution of Sm and Nd and partial re-equilibration of¹⁴³Nd/¹⁴⁴Nd ratios. This probably occurred during the upper granulite facies metamorphism which has also been dated at～ 3100m.y. by RbSr and UPb zircon studies [1]. Coexisting clinopyroxene, apatite and total rock fractions in two adjacent samples define an approximately linear array corresponding to an age of 2300 ± 300 m.y. This array indicates that redistribution of Sm and Nd and re-equilibration of¹⁴³Nd/¹⁴⁴Nd ratios occurred on an intermineral scale during the upper amphibolite to lower granulite facies metamorphism at～ 2450m.y.Due to the resetting of the SmNd system on both whole rock and mineral scales, the primary crystallization age of the igneous protolith is not well constrained by the present data, although it is clearly3100m.y. If it is assumed that the complex was derived initially from a depleted mantle reservoir(?_Nd(T) ? 2), evolution of the negative ?_Nd value of ?2.0 with the observed Sm/Nd ratios requires a prehistory of～ 380m.y. This implies a primary age of～ 3480m.y. However, substantially older primary ages can be inferred if the source reservoirs had?_Nd(T) > 2 and/or substantial reductions in the Sm/Nd ratio occurred in whole rocks during the granulite facies metamorphism at 3100 m.y. Such an inferred reduction in the Sm/Nd ratio may have been the result of preferential loss of Sm relative to Nd, or introduction of a low Sm/Nd fluid with?_Nd ≥ 0 during granulite facies metamorphism.     

U–Pb dating of silicic lavas,sills and syneruptive resedimented volcaniclastic deposits of the Lower Devonian Crudine Group,Hill End Trough,New South Wales

The Hill End Trough of central‐western New South Wales was an elongate deep marine basin that existed in the Lachlan Fold Belt from the early Late Silurian to late Early Devonian. It is represented by a regionally extensive, unfossiliferous sequence of interbedded turbidites and hemipelagites of substantially silicic volcanic derivation, which passes laterally into contemporaneous shallow‐water sedimentary rocks. The Turondale and Merrions Formations of the Lower Devonian Crudine Group are two prominent volcanogenic formations in the predominantly sedimentary trough sequence. They contain a range of primary and resedimented volcanic facies suitable for U–Pb dating. These include widespread subaqueous silicic lavas and/or lava cryptodomes, and thick sequences of crystal‐rich volcaniclastic sandstone emplaced by a succession of mass‐flows that were generated by interaction between contemporaneous subaerial pyroclastic flows and the sea. Ion microprobe dating of the two volcanogenic formations by means of the commonly used SL 13 zircon standard yields ages ranging between 411.3 ± 5.1 and 404.8 ± 4.8 Ma. Normalising the data against a different zircon standard (QGNG) yields preferred slightly older mean ages that range between 413.4 ± 6.6 and 407.1 ± 6.9 Ma. These ages broadly approximate the Early Devonian age that has been historically associated with the Crudine Group. However, the biostratigraphically inferred late Lochkovian ‐ early Emsian (mid‐Early Devonian) age for the Merrions Formation is inconsistent with the current Australian Phanerozoic Timescale, which assigns an age of 410 Ma to the Silurian‐Devonian boundary, and ages of 404.5 Ma and 395.5 Ma to the base and top of the Pragian, respectively. There is, however, good agreement if the new ages are compared with the most recently published revision of the Devonian time‐scale. This suggests that the Early Devonian stage boundaries of the Australian Phanerozoic Timescale need to be revised downward. The new ages for the Merrions Formation could also provide a time point on this time‐scale for the Pragian to early Emsian, for which no data are presently available.     

A comparison of reversing psychrometric Bowen ratio measurement systems: Research note

Abstract

Bowen ratio measurement systems that use a rotating apparatus for periodically reversing the psychrometers to remove systematic errors are compared in the laboratory and above a Douglas‐fir stand. The effect of some non‐cancelling errors on the measurement of the Bowen ratio and forest evapotranspiration is illustrated, and an improved apparatus and psychrometer are described.     

Stable Isotope Ratios: Hurricane Olivia

The oxygen and hydrogen isotopic compositions of rains from HurricaneOlivia (1994) in the eastern Pacific were measured. The rains werecollected on 24 and 25 September during airplane flights conducted at anelevation of 3 km. Hurricane Olivia peaked in intensity to a category-4storm between the two dates. Isotope ratios of rains from HurricaneOlivia were markedly lower ( ¹⁸O = –13.9to –28.8) than that of rain collected from a thunderstormat an elevation of 2.3 km outside the influence of Olivia (¹⁸O = –3.8). A distinct decrease in isotoperatios from the first day to the next ( ¹⁸O =–18.4 to –21.9) in Hurricane Olivia wasattributed to decreased updraft velocities and outflow aloft. Thisshifted the isotopic water mass balance so that fewer hydrometeors werelifted and more ice descended to flight level. A decrease in the averagedeuterium excess from the first day to the next (d = 15.5 to 7.1)was attributed to an increase in the relative humidity of the watervapor `source' area. We hypothesize that the `source' region for therain was in the boundary layer near the storm center and that becausethe hurricane was at peak intensity prior to the second day the relative humidity was higher.     

Condensed water in tropical cyclone “Oliver”, 8 February 1993

On February 8, 1993, the NASA DC-8 aircraft profiled from 10,000 to 37,000 feet (3.1–11.3 km) pressure altitude in a stratified section of tropical cyclone “Oliver” over the Coral Sea northeast of Australia. Size, shape and phase of cloud and precipitation particles were measured with a 2-D Greyscale probe. Cloud/ precipitation particles changed from liquid to ice as soon as the freezing level was reached near 17,000 feet (5.2 km) pressure altitude. The cloud was completely glaciated at −5°C. There was no correlation between ice particle habit and ambient temperature. In the liquid phase, the precipitation-cloud drop concentration was 4.0 × 10³ m⁻³, the geometric mean diameter D_g=0.5−0.7 mm, and the liquid water content 0.7−1.9 g m⁻³. The largest particles anywhere in the cloud, dominated by fused dendrites at concentrations similar to that of raindrops (2.5 × 10³ m⁻³) but a higher condensed water content (5.4 g m⁻³ estimated) were found in the mixed phase; condensed water is removed very effectively from the mixed layer due to high settling velocities of the large mixed particles. The highest number concentration (4.9 × 10⁴ m⁻³), smallest size (D_g=0.3−0.4 mm), largest surface area (up to 2.6 × 10² cm² m⁻³ at 0.4−1.0 g m⁻³ of condensate) existed in the ice phase at the coldest temperature (−40°C) at 35,000 feet (10.7 km). Each cloud contained aerosol (haze particles) in addition to cloud particles. The aerosol total surface area exceeded that of the cirrus particles at the coldest temperature. Thus, aerosols must play a significant role in the upscattering of solar radiation. Light extinction (6.2 km⁻¹) and backscatter (0.8 sr⁻¹ km⁻¹) was highest in the coldest portion of the cirrus cloud at the highest altitude.