Preservation of the shell matrix protein dermatopontin in 1500 year old land snail fossils from the Bonin islands

Organic molecules such as proteins can be preserved in certain fossils. The bulk properties of fossil proteins of both vertebrates and invertebrates have been studied for over half a century. Named proteins have so far been identified, however, only in vertebrate fossils, such as collagen from mammoth bones. Using immunological assays, we examined 1500 year old fossils of the extinct land snail Mandarina luhuana from the Bonin islands for the presence of dermatopontin, a molluscan shell matrix protein. First, we examined the shell microstructure and mineralogy of the fossil shells using scanning electron microscopy (SEM) and powder X-ray diffraction (XRD) in order to estimate the extent of diagenetic alteration. The results suggest that the original microstructure and mineralogy of the shells are preserved. Antiserum raised against the Type-1 dermatopontin fragment of the living land snail Euhadra brandtii showed significant immunological reactivity with the extracts from the fossil shells of M. luhuana. Immunological binding curves drawn for the shell extracts of extant M. aureola and the extinct M. luhuana confirmed the presence of dermatopontin in the fossil shells and provided an estimate that about 75–98% of the original dermatopontin was lost from the M. luhuana fossils. This is the first report of a named protein being identified in invertebrate fossils.     

Sedimentation of terrigenous red soils in coastal area adjacent to an estuary and coral reefs,the Okkubi River estuary,Okinawa Island,Japan

Sedimentation process of fine-grained terrigenous red soils was observed on the seafloor by coral reefs near an estuary using a paired mooring of turbidity meter and sediment trap or a mooring of sediment trap and current meter. Silty clays contained in the bottom calcareous sands were mainly resuspended and trapped in both surface and bottom layer traps, at noncatastrophic normal sedimentation periods. In addition, silty clays were supplied to this mooring site by inflowing river. Turbidity and the flux rate determined by sediment traps show certain relationship accompanied with the coefficient, which is changed by precipitation, current, and other conditions. Resuspension process caused by a typhoon was recorded as both core sequence of sediment trap and time-series data of bottom current. Maximum velocity of 49.5 cm/s was recorded from bottom currents resulting from the passing of attyphoon at the mooring site. A graded sand layer is interbedded in dark-gray, silty clay and considered to be a resuspended sediment resulting from the passing of the typhoon. Flux of the resuspended sediments by the typhoon was estimated from this core sequence and compared to the flux observed at the normal sedimentation.     

Temperatures and Oxygen Isotopic Compositions of Hydrothermal Fluids for the Takatori Tungsten-copper Deposit, Japan

Abstract. Fluid inclusion and oxygen isotope studies are performed to obtain temperatures and oxygen isotopic compositions of hydrothermal fluids for the vein-type tungsten-copper deposit at Takatori in Ibaraki Prefecture, Japan. Temperatures of the hydrothermal fluids are calculated from fluid inclusion data. The calculation incorporates the effects of the salinity, gas concentration, and fluid pressure. The fluid temperatures range from 370 to 460C. For these calculations, this study obtains a density equation for H₂O-NaCl-CO₂ solution at the vapor-liquid two-phase boundary. Then the present study combines the obtained equation with the equation of state by Bowers and Helgeson (1983).
The fluid temperatures determined in this study are applied to the calculation of oxygen isotopic compositions of the hydrothermal fluids. The calculation of the oxygen isotopic compositions is based on the oxygen isotope analyses of vein quartz. The oxygen isotopic compositions of vein quartz range from +13.5 to +14.4 % relative to SMOW. Then, the oxygen isotopic compositions of the hydrothermal fluids in equilibrium with the vein quartz are calculated to be from +9.7 to +10.5 %. These δ¹⁸O_fluid values agree with those of magmatic fluids derived from the ilmenite-series granitic rock, which is related to the mineralization. Keywords: Takatori tungsten-copper deposit, fluid inclusion, oxygen isotope, vein quartz, H₂O-NaCl-CO₂ solution, density     

Stage-V copepodites of Calanus sinicus and Calanus jashnovi (Copepoda: Calanoida) in mesopelagic zone of Sagami Bay as identified with genetic markers, with special reference to their vertical distribution

We applied genetic makers to identify Calanus species occurring in Sagami Bay, Japan, in order to investigate their vertical distribution in the upper 1000 m. First, interspecific genetic distances of three gene loci, mitochondrial small ribosomal RNA (srRNA), nuclear internal transcribed spacers 1 (ITS1) and 2 (ITS2), were estimated from morphologically distinguishable adult females of Calanus sinicus, Calanus jashnovi and Calanus pacificus that were collected from Sagami Bay, the Kuroshio Extension and the Oyashio region, respectively. The highest levels of interspecific genetic distance were observed in srRNA, followed by ITS1 and ITS2. The intraspecific genetic distances within C. sinicus were much lower than the interspecific genetic distances, indicating that DNA sequences in these loci are consistent with the morphological differences. This information was used as a criterion for species identification based on DNA sequence variation, and allowed us to identify the fifth copepodites (CVs) or younger stages of these species. Next, the vertical distribution of Calanus species was investigated in Sagami Bay in May 2006, on the basis of a stratified sampling in the upper 1000 m. By applying the genetic markers, 23 individuals comprising all copepodite stages were allocated into either C. sinicus or C. jashnovi, and the small- and large-sized CVs were identified as C. sinicus and C. jashnovi, respectively. The total abundance of C. sinicus was highest at 0-50 m and decreased with depth. On the contrary, CV individuals of C. sinicus were abundant not only in 0-50 m but also below 200 m with minimum occurrences in 150-200 m depth. C. jashnovi was much less abundant than C. sinicus and comprised of only CIV and CV which occurred in the upper 100 m and deeper than 50 m depths, respectively. The abundance of C. sinicus in the 1000-m water column of Sagami Bay was at a level comparable to that in shelf waters, suggesting the importance of off-shelf individuals in the biological production and organic transport in the respective areas.     

Vertical double silicate maxima in the sea-ice reduction region of the western Arctic Ocean: Implications for an enhanced biological pump due to sea-ice reduction

The R/V Mirai conducted hydrographic surveys in the western Arctic Ocean during summer 2004 (Mirai04) over wide east-west ranges from Alaska to eastern Siberia, where sea-ice cover has been greatly reduced in recent summers. The obtained data reveal differences in silicate profiles between shelf slope areas east and west of the Chukchi Plateau, the ridge that divides the Canada Basin into the Alaskan and east Siberian sides. East of the plateau, a single silicate maximum was found in a layer of Pacific-origin winter water, as examined in many previous studies. In contrast, west of the plateau, we found vertical double silicate maxima, which are reported for the first time in this study. The shallower silicate maximum corresponded to an N** minimum, signaling denitrification at the shelf bottom. This suggests that the shallower silicate maximum was caused by the spreading of shelf water. In contrast, the deeper silicate maximum corresponded to an oxygen minimum and a maximum silicate/phosphate ratio (Si/P), suggesting that this deeper maximum resulted from the decomposition of opal-shelled organisms. We also compared a silicate profile from Mirai04 to aprofile from the Arctic Ocean Section 94 (AOS94) expedition of 1994, a heavy ice year. The results suggest that sea-ice loss has enhanced biological activities, likely resulting in the appearance of the deeper silicate maximum.     

Melting relationships in the system Fe-Feo at high pressures: Implications for the composition and formation of the earth's core

A reconnaissance investigation has been carried out on melting relationships in the system Fe-FeO at pressures up to 25 GPa and temperatures up to 2200° C using an MA-8 apparatus. Limited studies were also made of the Co-CoO and Ni-NiO systems. In the system FeFeO, the rapid exsolution of FeO from liquids during quenching causes some difficulties in interpretation of textures and phase relationships. The Co-CoO and Ni-NiO systems are more tractable experimentally and provide useful analogues to the Fe-FeO system. It was found that the broad field of liquid immiscibility present at ambient pressure in the Co-CoO system had disappeared at 18 GPa, 2200° C and that the system displayed complete miscibility between molten Co and CoO, analogous to the behaviour of the Ni-NiO system at ambient pressure. The phase diagram of the system Fe-FeO at 16 GPa and from 1600–2200° C was constructed from interpretations based on the textures of quenched run products. The solubility of FeO in molten iron is considerably enhanced by high pressures. At 16 GPa, the Fe-FeO eutectic contains about 10–15 mol percent FeO and the eutectic temperature in this iron-rich region of the system occurs at 1700±25° C, some 350° C below the melting point of pure iron at the same pressure. The solubility of FeO in molten Fe increases rapidly as temperature increases from 1700 to 2200° C. A relatively small liquid immiscibility field is present above 1900° C but is believed to be eliminated above 2200° C. This inference is supported by thermodynamic calculations on the positions of key phase boundaries. A single run carried out on an Fe₅₀ FeO₅₀ composition at 25 GPa and 2200° C demonstrated extensive and probably complete miscibility between Fe and FeO liquids under these conditions. The melting point of iron is decreased considerably by solution of FeO at high pressures; moreover, the melting point gradient (dP/dT) of the Fe-FeO eutectic is much smaller than that of pure iron and is also smaller than that of mantle pyrolite under the P, T conditions studied. These characteristics make it possible for melting of metal phase and segregation of the core to proceed within the Earth under conditions where most of the mantle remains below solidus temperatures. Under these conditions, the core would inevitably contain a large proportion of dissolved FeO. It is concluded therefore, that oxygen is likely to be the principal light element in the core. The inner core may not be composed of pure iron, as often proposed. Instead, it may consist of a crystalline oxide solid solution (Ni, Fe)₂O.     

Oxygen isotope exchange rate between dissolved sulfate and water at hydrothermal temperatures

Oxygen isotope exchange rate between dissolved sulfate and water was experimentally determined at 100, 200 and 300°C. The isotope exchange rate is strongly dependent on temperature and pH of the solution. Combining the temperature and pH dependence of the reaction rate, the exchange reaction was estimated to be first-order with respect to sulfate. The logarithm of apparent rate constant of exchange reaction at a given temperature is a function of the pH calculated at the experimental temperatures. From the pH dependence of the apparent rate constant, it was deduced that the isotope exchange reaction between dissolved sulfate and water proceeds through collision between H₂SO⁰₄ and H₂O at low pH, and between HSO^?₄ and H₂O at intermediate pH. The isotope exchange rate obtained indicates that oxygen isotope geothermometry utilizing the studied isotope exchange is suitable for temperature estimation of geothermal reservoirs. The extrapolated half-life of this reaction to oceanic temperature is about 10⁹ years, implying that exchange between oceanic sulfate and water cannot control the oxygen isotope ratio of oceanic sulfates.     

Zinc-rich Pyrite from the TAG Active Mound, the TAG Hydrotherma Field, Mid-Atlantic Ridge

Abstract: Pyrite rich in Zn, up to 3.1 wt%, was found in the TAG active mound of the TAG hydrothermal field, the slow-spreading Mid-Atlantic Ridge at 26°08'N and 44°49'W. The Zn-rich pyrite is characterized by an optical homogeneity, a homogeneous distribution of Zn in the back-scattered electron images, both at a magnification of about 500, a negative correlation between Fe and Zn contents of the pyrite and a rather small unit cell edge (a₀ = 5.4117 ± 0.0008Å), strongly indicating that the detected Zn is present in the pyrite in solid solution. Such Zn concentrations are observed exclusively in dendritic pyrite, suggesting that the Znrich pyrite grew from hydrothermal fluids of a high degree of supersaturation due to quenching on the seafloor.