4-tert-octylphenol and 17beta-estradiol applied by feeding to flounder Platichthys flesus: induction of vitellogenin and accumulation in tissues

In order to simulate a possible natural administration route of xenoestrogens male flounder Platichthys flesus were exposed via the diet to the alkylphenol 4-tert-octylphenol. Treatment with 4-tert-octylphenol (10, 50 and 100 mg OP kg(-1) BW) or 17beta-estradiol (0.05 mg kg(-1) BW) every second day during a period of 11 days resulted in a significant increase in plasma vitellogenin (Vtg) concentrations. The induction of Vtg was greatest in the fish receiving 50 mg OP kg-' BW. A significant accumulation of 4-tert-octylphenol was found in liver and muscle tissue of the OP treated groups. The tissue concentrations of 4-tert-octylphenol and the plasma vitellogenin concentration were positively correlated. The results show that 4-tert-octylphenol accumulates in liver and muscles of flounder P. flesus, and exerts estrogenic effects such as vitellogenin induction.     

The radiation balance at Copenhagen

Summary At the Climate and Water Balance Station of the Danish Hydrotechnical Laboratory near Copenhagen incoming radiation has been recorded continuously since 1954 by means of Moll thermopiles in Kipp & Zonen solarimeters and further, since 1955, reflected radiation, too. Since 1956 also net radiation and net long-wave radiation has been recorded by means of thermopiles in heat flow transducers from Beckman & Whitley used as radiometers.Mean daily values are given for incoming radiation as well as monthly mean values for reflected radiation, net long-wave radiation and net radiation. Recorded and calculated values of net long-wave radiation are given and discussed.

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Zusammenfassung An der Klima- und Wasserhaushaltsstation des Dänischen Hydrotechnischen Laboratoriums bei Kopenhagen wurde seit 1954 dauernd die Einstrahlung mittels Moll-Thermosäulen in Kipp & Zonen-Solarimetern registriert und außerdem seit 1955 die Albedo. Seit 1956 wurden auch die Strahlungsbilanz und die effektive Ausstrahlung mittels Thermosäulen in Beckman & Whitley-Wärmestrommessern, die als Strahlungsmesser verwendet wurden, registriert.In einigen Tabellen werden tägliche Mittelwerte der Einstrahlung mitgeteilt wie auch Monatsmittelwerte der Reflexstrahlung, der effektiven Ausstrahlung und der Strahlungsbilanz. Ferner werden beobachtete und berechnete Werte der langwelligen Strahlungsbilanz angeführt und diskutiert.

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Résumé On a effectué des enregistrements du rayonnement incident depuis 1954 et de l'albédo depuis 1955 à la Station d'économie climatologique et hydrologique du laboratoire danois d'hydrotechnique près de Copenhague. Le bilan radiatif fut également mesuré depuis 1956 ainsi que le rayonnement émis effectif.Les auteurs donnent ici des tableaux de valeurs journalières moyennes du rayonnement incident, des moyennes mensuelles de l'albédo, du rayonnement effectif et du bilan radiatif. Enfin des valeurs enregistrés et calculés du rayonnement effectif, sont présentés et discutés.

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With 5 Figures     

Livelihood diversification: continuity and change in the Sahel

Livelihood diversification can be crucial for poor rural households in the African Sahel. Migration is a common diversification strategy during the dry season, but there are also areas with significant strategies on the farm, such as incomes from gum and resins. Sudan is a country where the income from gum arabic, the gum from the Acacia senegal tree, has played a large economic role for smallholders for generations, but there are signs of a declining production which is detrimental if people have no alternative incomes. The results showed that in parts of the country, the 1984 drought caused an event-driven change resulting in a discontinuation of production. However, in other parts of the country, the agricultural system providing gum arabic proved to be more resilient to the drought and the incomes from gum arabic still play a large role. The results illustrated a complexity of driving forces, regional differences and a large variability in incomes between households. Some causes were indirect such as prices, drought, precipitation and locust, whereas the others were direct and hence more controllable by households. This study underlined the direct causes, such as how labour input is prioritised between livelihood activities, which have not been given as much attention in previous literature. Secondly, the need for a holistic view of livelihoods is underlined in order to understand the future of gum arabic production. Empirical data were collected from extensive fieldwork.

Amphipod prey of gray whales in the northern Bering Sea: Comparison of biomass and distribution between the 1980s and 2002–2003

The ampeliscid amphipod community in the Chirikov Basin of the northern Bering Sea was a focus of study during the 1980s because they were a major food for the Eastern North Pacific (ENP) population of gray whales Eschrichtius robustus. Information from the 1980s benthic investigations, published accounts of ENP gray whale population trends and the occurrence in 1999–2000 of an unusual number of gray whale mortalities prompted concern that the whale population may have exceeded the carrying capacity of its food base. Therefore, during two cruises per year between June and September, 2002 and 2003, we resampled the 20 stations occupied during the 1980s, to determine if there had been any significant changes in ampeliscid abundance and biomass. During 2002–2003, average ampeliscid dry weight biomass was about 28±10 g m⁻² (95% confidence interval), a decline of nearly 50% from maximum values in the 1980s. Amphipod length measurements indicated that the declines were due mainly to the absence of the larger animals (20–30 mm length). Two hypotheses were considered regarding the amphipod declines: gray whale predation and climate. Ampeliscid production (105 kcal m⁻² yr⁻¹) and gray whale energy requirements (1.6×10⁸ kcal individual⁻¹ yr⁻¹) indicated that as little as 3–6% of the current estimate of the ENP gray whale population could remove 10–20% of the annual ampeliscid production from the study site in 2002–2003, a finding consistent with the hypothesis that top-down control by foraging whales was the primary cause of the observed declines. A 10-yr time series of temperature near the bottom in the Bering Strait and northward transport did not reveal a consistent trend between 1990 and 2001, suggesting that climate influences were not the major cause of the observed declines. Arctic ampeliscids have slow growth rates and long generation times; therefore the ampeliscid community may require decades to recover to densities observed in the 1980s. Predicted warming trends in the northern Bering Sea could impact ampeliscid recovery by lowering primary production or altering the community composition of the benthos.     

Inter-hemispheric mesospheric coupling in a comprehensive middle atmosphere model

Observations of noctilucent clouds have revealed a surprising coupling between the winter stratosphere and the summer polar mesopause region. In spite of the great distance involved, this inter-hemispheric link has been suggested to be the principal reason for both the year-to-year variability and the hemispheric differences in the frequency of occurrence of these high-altitude clouds. In this study, we investigate the dynamical influence of the winter stratosphere on the summer mesosphere using simulations from the vertically extended version of the Canadian Middle Atmosphere Model (CMAM). We find that for both Northern and Southern Hemispheres, variability in the summer polar mesopause region from one year to another can be traced back to the planetary-wave flux entering the winter stratosphere. The teleconnection pattern is the same for both positive and negative wave-flux anomalies. Using a composite analysis to isolate the events, it is argued that the mechanism for inter-hemispheric coupling is a feedback between summer mesosphere gravity-wave drag (GWD) and zonal wind, which is induced by an anomaly in mesospheric cross-equatorial flow, the latter arising from the anomaly in winter hemisphere GWD induced by the anomaly in stratospheric conditions.     

Oral single pulse exposure of flounder Platichthys flesus to 4-tert-octylphenol: Relations between tissue levels and estrogenic effects

The accumulation of 4-tert-octylphenol and the associated estrogenic effects were studied after a single pulse exposure to flounder Platichthys flesus. 4-tert-octylphenol was administered orally in a single dose of 50 mg kg(-1) and tissue (liver, muscle and testis) and plasma concentrations of 4-tert-octylphenol as well as plasma vitellogenin were measured 3, 6, 12, 18, 24, 48, 72, 144 and 216 h after administration of the dose. Concentrations of 4-tert-octylphenol in plasma and tissues were determined by Liquid Chromatography Mass Spectrometry (LC-MS). 4-tert-octylphenol was detectable in liver, testis, muscle and plasma 3h post administration and an accumulation was observed in liver, muscle and plasma up to 12 h and in testis 18 h post administration, respectively. The maximum concentrations of 4-tert-octylphenol in liver, muscle and testis were 67, 3.2 and 6.8 microg g(-1), respectively. An increase in plasma vitellogenin levels was seen 48 h post administration and the vitellogenin level continued to increase (from <100 ng ml(-1) to 1.4 mg ml(-1)) until the end of the experiment 9 days after the administration of 4-tert-octylphenol.     

Chronic exposure of adults and embryos of Pandalus borealis to oil causes PAH accumulation, initiation of biomarker responses and an increase in larval mortality

Adult shrimps (Pandalus borealis) and their embryos were exposed to an oil-water dispersion (OWD) at concentrations of 0.015, 0.06 and 0.25 mg/L using a continuous flow system. Lysosomal membrane stability was analysed in haemocytes using the neutral red retention assay and an alkaline unwinding assay was used to measure DNA damage in hepatopancreas tissue. Exposure to oil induced concentration and time dependent biomarker responses in adult shrimps together with the accumulation of PAH in their tissues. Oil exposure of shrimp embryos caused increased mortality in the resultant larvae, even if the larvae were kept in clean water after hatching. There were minor differences observed in larval stage development times in the first part of the experiments. The fatty acid composition of embryos exposed to oil was different to that of non-exposed larvae. PAH tissue concentration and biomarker responses correlated to the reduced survival of the shrimp larvae.