Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

Biodiversity and studies of marine symbiotic siphonostomatoids off South Africa

Current knowledge of the biodiversity of the symbiotic marine siphonostomatoids from South African waters (136 species) is sparse compared to that globally (1 388 species). The difference is especially apparent when taking into account the diversity of fish (more than 2 000 species) and invertebrates (approximately 12 914 species) found in South African waters, all of which are all potential hosts of symbiotic siphonostomatoids. Most reports of symbiotic siphonostomatoids from South African waters are from Chondrichthyes and represent 80% of the currently known families and about 66% of the known genera. Taxonomic studies on the siphonostomatoids also provide information about their morphology, biology, habitat preference and host association, as well as the distribution of species and their associated host taxa. Molecular analysis, especially sequencing, provides additional information on the existence of cryptic species and population structure, but has high financial costs and a very low success rate in DNA isolation and PCR amplification. Reported declines in abundance of piscivorous fish species, for example, as a result of fishing pressure, with possible synergistic effects of environmental changes, and also the reported potential effects of anthropogenic and climate change on the broader coastal biota, may result in the loss of siphonostomatoid species before they can be described. Furthermore, in South Africa, there is a lack of professional taxonomists to study poorly known groups and this situation will not improve while research emphasis remains on commercially important groups and while taxonomic studies are not recognised for their value in research programmes.     

ASD field hyperspectral measurements for discrimination of the ferruginous rocks and the iron ore types at El Gedida-Ghorabi area,Bahariya Oasis,Western Desert,Egypt

The ironstone succession at El Gedida-Ghorabi-Naser area of El Bahariya depression is subdivided into lagoonal manganiferous mud and fossiliferous ironstone consisting mainly of hematite and goethite-hydrogoethite. The application of the ASD field spectroradiometer measurements (spectral range) in the ASTER data led to the interpretation of the presence of ferruginous units as quartzitic sandstone, gluconitic sandy clay, and pink marly limestone. The existing iron ore minerals in the iron ore localities were also classified into high Mn hematite, low Mn hematite, goethite, hydrogoethite as well as low- and high-grade Hematite and Barite. Quartz, feldspars, rutile, and clay minerals (e.g., kaolinite and illite) are mainly associated with the iron ore. Accessory minerals of manganese, e.g., psilomelane and pyrolusite, were also present. The Barite mineral is recorded as a common mineral association with the iron ore deposits at El Gedida and Ghorabi localities. The stratigraphical units investigated in the study area include the oldest gravely clayey sandstones of the Bahariya Formation overlain by the fossiliferous and oolitic limestones of the El-Hamra, Qazzun, and Naqb Formations. Quartztic sandstones and clayey sandstones of the Radwan Formation and youngest Quaternary sediments of sandy-clayey materials were often found as intermittent cover and overburden in unconformity surfaces over the iron ore bands.     

Hepatic CYP1A levels and EROD activity in English sole: biomonitoring of marine contaminants in Vancouver Harbour

To assess chemical contaminant stress in the marine environment, ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) expression were measured in 88 English Sole (Pleuronectes vetulus) collected during May and June 1999 from four sites in Vancouver Harbour and at an expected reference site outside the harbour. Hepatic microsomes were prepared from the fish and analyzed for total CYP content, EROD activity, and CYP1A protein levels. Hepatic EROD activity and CYP1A protein levels were elevated in fish from two sites in the inner harbour. A comparison with sediment chemistry data showed that fish with increased EROD activity and CYP1A levels came from sites containing relatively high levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls. Unexpectedly high levels of EROD activity and CYP1A protein were also found in fish from a reference site near Gibsons, in Howe Sound. The elevated EROD activity and CYP1A expression in fish from this site cannot be explained by the chemical analysis data collected.     

Multiple wavelength observations of a solar active region

The Solar Maximum Mission Satellite, the Sacramento Peak Vacuum Tower Telescope, the Very Large Array and the Westerbork Synthesis Radio Telescope have been used to observe active region AR 2490 on two consecutive days at soft X-ray, ultraviolet, optical and radio wavelengths (2, 6, and 20 cm), with comparable angular resolution (2 to 15) and field of view (4 × 4). The radio emissions at = 6 cm and 20 cm show a double structure in which one component is associated with bright H plage, C iv and soft X-ray emission, and the other component is associated only with sunspots. No radiation at = 2 cm is detected in this latter component. Coronal temperature and emission measure derived from X-ray lines indicate that the dominant radiation mechanism of the plage-associated component is due to thermal bremsstrahlung while the gyroresonance absorption coefficient must be invoked to account for the high brightness temperature (T _b 2×10⁶K) observed in the sunspot associated component. The high magnetic field strength needed (600 G at a level where T2×10⁶K) is explained assuming a thin transition zone, in order to reach a high electron temperature close to the sunspot, where the magnetic fields are stronger. A higher temperature gradient above sunspots is also consistent with the absence of detectable C iv emission.Cooperative study of the SMY-FBS Project.On leave from the University of Napoli.On leave from the University of Torino.On sabbatical leave 1980–81 at the Arcetri Observatory.On leave from Toyokawa Observatory, Japan.     

Siphonostomatoid copepods infecting Squalus acutipinnis Regan, 1908 off South Africa

Squalus acutipinnis (previously included in S. megalops) individuals caught off the east, south and west coasts of South Africa were examined for siphonostomatoid copepod infection. Collected siphonostomatoids were fixed and preserved in 70% ethanol and studied under microscopes using both transmitted and incidental light. The copepods were identified and the host-parasite associations estimated by calculating their prevalence, mean intensity and mean abundance. The siphonostomatoids represented four families and six species, and included new host records for Nemesis sp., Lernaeopoda sp. and Eudactylina acanthii, and a new geographical record for E. acanthii. Squalus acutipinnis individuals were infected by relatively low loads of siphonostomatoids. The most commonly found species was Achtheinus pinguis, the only species found off all three coasts. There was no difference in the prevalence of A. pinguis in sharks sampled off the three coasts but the highest mean intensity was found on the East Coast followed by the West Coast. The overall prevalence of A. pinguis was 33.6%, and was an order of magnitude higher than that of the other siphonostomatoids recorded. At such low levels of infection it will thus be necessary to examine a large number of hosts to determine the biodiversity of siphonostomatoids and geographical differences in prevalence in South African waters.     

Geomatics Based Analysis of Predicted Sea Level Rise and its Impacts in Parts of Tamil Nadu Coast,India

The coastal zones around the world are very densely populated and hence heavily packed with related infrastructures. So, the territorial nations have obvious apprehensions against the IPCC SRES (Intergovernmental Panel on Climate Change, Special Report on Emission Scenario) predicted sea level rise, as it would cause flooding of the low lying coasts and also other related chains of environmental endangers. This has driven these nations to initiate research studies in multiple directions for scientifically evaluating the phenomenon and impacts of sea level rise using all possible technologies including the Geomatics which possesses unique credentials in geosystem mapping. But certain advanced virtues available with Geomatics technology are yet to be capitalized deservingly in this. In addition, almost all the earlier studies have focused only on the impacts of sea level rise (SLR) and not on the predicted shift of high tide line (HTL) and the related inter tidal activities, which would cause a series of environmental disaster. Hence, the present research study was undertaken in a test site of 750 km² in central Tamil Nadu coast to visualize the areas prone to submergence due to predicted SLR and areas prone to environmental disasters/degradation viz. erosion, deposition, salination of agricultural lands, pollution of aquifers, etc. due to predicted shift of HTL, using digital elevation models derived from SRTM data (Shuttle Radar Topographic Mission), geomorphology and land use/cover maps interpreted using IRS P6 LISS IV satellite data. The paper narrates the certain newer concepts and methodologies adopted in the study and the results.     

Slicks detection on the sea surface based upon polarimetric SAR data

This letter proposes a generalized-likelihood ratio test-based edge detector to be fed by possibly polarimetric synthetic aperture radar (SAR) data. It can be used to detect dark spots on the sea surface and, hence, as the first stage of a system for identification and monitoring of oil spills. The proposed constant false alarm rate (CFAR) detector does not require secondary data (namely pixels from a slick-free area); remarkably, a preliminary performance assessment, carried out by resorting to real SAR recordings, shows that it guarantees detection capabilities comparable to those of previously proposed polarimetric CFAR detectors (which though make use of secondary data). The preliminary performance assessment also seems to indicate that processing polarimetric data does not ensure improved detection capabilities.     

Photo-identification of bottlenose dolphins in the far south of New Zealand indicates a ‘new’, previously unstudied population

Declines in the abundance of bottlenose dolphins in the Bay of Islands and in Doubtful Sound have contributed to the species being classified as Nationally Endangered in New Zealand waters. Updated information on distribution and abundance nationwide is therefore a high priority. This study presents data from the first photo-identification surveys of Paterson Inlet, Stewart Island, conducted to document bottlenose dolphin presence, abundance and residency. Open-population mark-recapture models indicate that 18 (95%CI = 15–20) dolphins regularly use Paterson Inlet. Photo-identification of dolphins from unknown populations during two chance encounters in Otago Harbour and Dusky Sound included 11 individuals previously identified at Stewart Island. These results indicate that dolphins found regularly at Stewart Island are part of a larger, wide ranging southern population with a minimum population abundance of 92 (95%CI = 80–111) individuals.     

Morphometric variation in the cutlassfish Trichiurus lepturus on the Kenyan coast: implications for stock identification and management

Morphometric variation was used to study population structure of the cutlassfish Trichiurus lepturus on the Kenyan coast. In all, 16 morphometric measurements taken from 193 individuals from six sites were subjected to multivariate statistical analysis in order to determine possible stock differentiation. Multivariate analysis of variance indicated significant differences in the means of a combination of the morphometric characters among the sites, and discriminant function analysis by groups showed the presence of phenotypically distinct populations on the south and north coasts of Kenya. A classification tree analysis of morphometric characters validated the distinct groups and indicated certain characters to be useful for classifying specimens to source sites. The likely presence of distinct north and south stocks of T. lepturus on the Kenyan coast observed in our study will require validation by other methods, but indicates the potential need for spatially explicit models for managing fish populations on a large spatial scale.