棚洞结构洞形优化计算分析

随着国民经济的发展和社会的进步,公路隧道环保型建设技术日益受到重视。以南京-淮安高速公路南京江北段老山公路隧道为依托,提出了一种环保型结构--新型棚洞。采用大型通用有限元软件ANSYS对5种不同棚洞结构型式进行了优化。从弯矩、轴力、剪力3个方面综合计算分析表明,全拱式棚洞是最优方案。     

深海羽毛降解菌Bacillus sp. JM7极端碱耐受角蛋白酶的酶活性质研究

Bacillus sp. JM7, a strain isolated from the deep-sea of the South China Sea, was found to efficiently degrade 79.4% native chicken feather within 30 h. Scanning electron microscopy analysis showed that JM7 strain could gradually degrade feather by modifying the microstructure of feather keratin. A total of 25 protease genes were predicted from the draft genome of JM7 strain, among which a predicted subtilisin-like serine protease(designated as Ker02562) was further characterized for its keratinolytic activity. The recombinant Ker02562 functioned at a wide range of temperatures from 30℃ to 60℃, with an optimum at 40–50℃. Ker02562 was highly active at various pHs ranging from 5.0 to 13.0, with a maximum activity observed at pH 7.0–9.0. Remarkably, recombinant Ker02562 was stable in extreme alkaline environments(pH 10–13), which was much better than most other reported keratinases. Collectively, these favorable properties could make Bacillus sp. JM7 and Ker02562 attractive to be applied in the detergent formulation and feather bioconversion.     

水下环肋圆柱壳弹性失稳临界载荷无损预报方法

基于振动的水下环肋圆柱壳临界载荷的预报具有结构无损的优势。以Flügge壳体理论和基于正交各向异性理论的环肋圆柱壳自由振动方程为基础,运用波传播法得出耦合系统的频率方程,并求出对应外压下的固有频率,通过最小二乘法线性拟合获得临界载荷、外压及固有频率的关系表达式。绘图得知任意边界的同一模态下,水下环肋圆柱壳的固有频率平方与静水压力成正比。结构失稳时,刚度丧失,固有频率降为零,据此求出水下环肋圆柱壳的最小弹性临界载荷。分析了不同边界条件对环肋圆柱壳临界载荷的影响,得知边界约束越强,环肋圆柱壳的临界载荷越大。结果对比表明了本方法的正确性,为水下环肋圆柱壳的固有频率及临界载荷的理论计算提供了一种新的方法。