Biotite weathering and nutrient uptake by ectomycorrhizal fungus, Suillus tomentosus, in liquid-culture experiments

Ectomycorrhiza-forming fungi (EMF) alter the nutrient-acquisition capabilities of vascular plants, and may play an important role in mineral weathering and the partitioning of products of weathering in soils under nutrient-limited conditions. In this study, we isolated the weathering function of Suillus tomentosus in liquid-cultures with biotite micas incubated at room temperature. We hypothesized that the fungus would accelerate weathering by hyphal attachment to biotite surfaces and transmission of nutrient cations via direct exchange into the fungal biomass. We combined a mass-balance approach with scanning electron microscopy (SEM) and atomic force microscopy (AFM) to estimate weathering rates and study dissolution features on biotite surfaces. Weathering of biotite flakes was about 2-3 orders of magnitude faster in shaken liquid-cultures with fungus compared to shaken controls without fungus, but with added inorganic acids. Adding fungus in nonshaken cultures caused a higher dissolution rate than in inorganic pH controls without fungus, but it was not significantly faster than organic pH controls without fungus. The K⁺, Mg²⁺ and Fe²⁺ from biotite were preferentially partitioned into fungal biomass in the shaken cultures, while in the nonshaken cultures, K⁺ and Mg²⁺ was lost from biomass and Fe²⁺ bioaccumulated much less. Fungal hyphae attached to biotite surfaces, but no significant surface changes were detected by SEM. When cultures were shaken, the AFM images of basal planes appeared to be rougher and had abundant dissolution channels, but such channel development was minor in nonshaken conditions. Even under shaken conditions the channels only accounted for only 1/100 of the total dissolution rate of 2.7 × 10⁻¹⁰ mol of biotite m⁻² s⁻¹. The results suggest that fungal weathering predominantly occurred not by attachment and direct transfer of nutrients via hyphae, but because of the acidification of the bulk liquid by organic acids, fungal respiration (CO₂), and complexation of cations which accelerated dissolution of biotite. Results further suggest that both carbohydrate source (abundant here) and a host with which nutrients are exchanged (missing here) may be required for EMF to exert an important weathering effect in soils. Unsaturated conditions and physical dispersal of nutrient-rich minerals in soils may also confer a benefit for hyphal growth and attachment, and promote the attachment-mediated weathering which has been observed elsewhere on soil mineral surfaces.     

The spacetime gravitational field of a deformable body

The high-resolution analysis of orbit perturbations of terrestrial artificial satellites has documented that the eigengravitation of a massive body like the Earth changes in time, namely with periodic and aperiodic constituents. For the space-time variation of the gravitational field the action of internal and external volume as well as surface forces on a deformable massive body are responsible. Free of any assumption on the symmetry of the constitution of the deformable body we review the incremental spatial (“Eulerian”) and material (“Lagrangean”) gravitational field equations, in particular the source terms (two constituents: the divergence of the displacement field as well as the projection of the displacement field onto the gradient of the reference mass density function) and the `jump conditions' at the boundary surface of the body as well as at internal interfaces both in linear approximation. A spherical harmonic expansion in terms of multipoles of the incremental Eulerian gravitational potential is presented. Three types of spherical multipoles are identified, namely the dilatation multipoles, the transport displacement multipoles and those multipoles which are generated by mass condensation onto the boundary reference surface or internal interfaces. The degree-one term has been identified as non-zero, thus as a “dipole moment” being responsible for the varying position of the deformable body's mass centre. Finally, for those deformable bodies which enjoy a spherically symmetric constitution, emphasis is on the functional relation between Green functions, namely between Fourier-/ Laplace-transformed volume versus surface Love-Shida functions (h(r),l(r) versus h ^′(r),l ^′(r)) and Love functions k(r) versus k ^′(r). The functional relation is numerically tested for an active tidal force/potential and an active loading force/potential, proving an excellent agreement with experimental results. Received: December 1995 / Accepted: 1 February 1997     

Organic matter in a low productivity anoxic intraplatform basin in the Triassic Tethys

This paper presents isotopic, bulk geochemical and biomarker data measured on organic matter accumulated in a narrow extensional basin developed at the oceanward margin of the huge Triassic carbonate platform in the Alps–Appenines domain. The integration of isotope signatures, organic petrographical and biomarker evidence together with the composition of kerogen pyrolysates suggests immature organic matter predominantly of algal origin with a minor, but not negligible, higher plant derived and moderate bacterial contribution for the entire sequence. The mineral sources are dominated by platform-derived subtidal Dachstein limestone with a minor palaeosol input and a moderate contribution of autochtonous quartz. Nevertheless, parallel variations observed in the mineral content, as well as in the amount and the quality of the organic matter reflect variations in the palaeoenvironment. The increased humidity, existing in the period of the accumulation of the upper section of the sequence, led to the restriction of dolomitization. A slightly greater higher plant derived contribution, in this section, is evidenced by the composition of bitumen and the results on GC and GC/MS analyses on the non-aromatic hydrocarbon fraction of bitumen. Moreover, the climate-induced weathering enchanced the primary productivity and resulted in a pronounced increase in the TOC content. The average estimated value of the planktonic productivity is about three times higher for the calcite-rich sequence than the dolomite-rich one, being 44.2 and 15.3 tC_org/m²/Ma, respectively. The low to moderate planktonic productivity shows that anoxic conditions, observed for the entire succession, are a consequence of the stagnant water stratification rather than high planktonic productivity. Depth trends in the data measured on kerogens (HI, OI, δ¹³Corg values, composition of pyrolysate) together with the δ¹⁸O excursions and Δδ¹³C values appear to be controlled by sea-level fluctuations. Consistent with the high abundance of alkyltiophenes in kerogen pyrolysates, the high S_org/C ratios (ranging between 0.05 and 0.10) suggest the importance of natural sulfurization in the formation of the sulfur-rich type-II-S kerogen occurring in all of the samples.     

Accessing effects and signals of leakage from a CO<Subscript>2</Subscript> reservoir to a shallow freshwater aquifer by reactive transport modelling

CO₂ geological storage is a transitional technology for the mitigation of climate change. In the vicinity of potential CO₂ reservoirs in Hungary, protected freshwater aquifers used for drinking water supplies exist. Effects of disaster events of CO₂ escape and brine displacement to one of these aquifers have been studied by kinetic 1D reactive transport modelling in PHREEQC. Besides verifying that ion concentrations in the freshwater may increase up to drinking water limit values in both scenarios (CO₂ or brine leakage), total porosity of the rock is estimated. Pore volume is expected to increase at the entry point of CO₂ and to decrease at further distances, whereas it shows minor increase along the flow path for the effect of brine inflow. Additionally, electrical conductivity of water is estimated and suggested to be the best parameter to measure for cost-effective monitoring of both worst-case leakage scenarios.     

Dependence of the Love numbers upon the inner structure of the Earth and comparison of theoretical models with results of measurements

Summary The question this paper is examining is the following: to what extent are the Love numbers dependent on certain characteristics of the inner structure of the Earth? It has been proven — on the basis of calculations carried out by the author-that these quantities are only in a small degree dependent on the density values measured on the surface of the Earth and on the selection of the density function in the mantle of the Earth. On the other hand the value of Love numbersh, k andl is considerably influenced by the assumptions made about the core of the Earth, namely by the position of the boundary between the core and the mantle and by the magnitude of the rigidity coefficient presumed in the core in the vicinity of the core-mantle boundary.The results of the calculations are compared with those mean values of Love numbers obtained from the data of stations operating at different places of the Earth. By reason of this it can be assumed that the core of the Earth has, in the vicinity of the core-mantle boundary, a coefficient of effective rigidity of the order of 10¹⁰ dyn/cm², if the core-mantle boundary is placed at the relative Earth radius of 0.545 from the centre of the Earth.     

Comment on the paper “Fast tidal cycling and the origin of life” by Richard Lathe

We show that the fast tidal cycling postulated by Lathe [Lathe, R., 2004. Icarus 168, 18-22] is not a plausible mechanism to explain the origin of life on Earth about 3.9 Ga ago. The value of LOD at this remote epoch was probably comprised between 15 to 17 h, and the Earth-Moon distance was only about 20% smaller than nowadays, implying that the tidal frequencies and amplitudes were not so dramatically different from the present ones as stated in Lathe's paper.     

Correlations of natural radionuclides in sediment from Danube

The correlation between specific activities of some natural radionuclides (²³⁸U, ²²⁶Ra, ²³²Th, ⁴⁰K) measured in sediment taken from river bottom was studied. The sediment was taken from the Serbian part of the Danube River. Good correlation between some of the isotopes is observed, so that their specific activity ratios are spread over a lower range than specific activities themselves. This suggests that evaluation of specific activity ratios of some natural radionuclides could be a more sensitive method for the determination of increased levels of some of them than the straightforward analysis of specific activities.     

The temporal variation of the spherical and Cartesian multipoles of the gravity field: the generalized MacCullagh representation

 The Cartesian moments of the mass density of a gravitating body and the spherical harmonic coefficients of its gravitational field are related in a peculiar way. In particular, the products of inertia can be expressed by the spherical harmonic coefficients of the gravitational potential as was derived by MacCullagh for a rigid body. Here the MacCullagh formulae are extended to a deformable body which is restricted to radial symmetry in order to apply the Love–Shida hypothesis. The mass conservation law allows a representation of the incremental mass density by the respective excitation function. A representation of an arbitrary Cartesian monome is always possible by sums of solid spherical harmonics multiplied by powers of the radius. Introducing these representations into the definition of the Cartesian moments, an extension of the MacCullagh formulae is obtained. In particular, for excitation functions with a vanishing harmonic coefficient of degree zero, the (diagonal) incremental moments of inertia also can be represented by the excitation coefficients. Four types of excitation functions are considered, namely: (1) tidal excitation; (2) loading potential; (3) centrifugal potential; and (4) transverse surface stress. One application of the results could be model computation of the length-of-day variations and polar motion, which depend on the moments of inertia. Received: 27 July 1999 / Accepted: 24 May 2000