Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus

A strain of thermophilic fungus Aspergillus fumigatus was cultured with K-bearing minerals to determine if microbe-mineral interactions enhance the release of mineralic potassium. Experiments were carried out in two settings, one with the mineral grains and the fungal cells in direct contact, and the other employing a membrane (pore size 0.22 μm) to separate the two. Measurements over a period of 30 days showed that, irrespective of the experimental setup, the concentration of free K in the culture was drastically higher than those in any of the control experiments where no living organism was present. Moreover, the occurrence of mineral-cell physical contact enhanced potassium release by an additional factor of 3 to 4 in comparison to the separation experiments. For contact experiments, Electron Probe Microanalysis revealed the formation of mycelium-mineral aggregates, and Atomic Force Microscopy imaging further indicated the possible ingestion of mineral particles by the fungus cells. Contrasting to what was observed and expected in control experiments, the potassium solubilization rate showed a positive dependence upon pH when fungi and minerals were mixed directly, and exhibited no correlations with solution acidity if cell-rock contact was restrained. These results appear to suggest that A. fumigatus promoted potassium release by means of at least three likely routes, one through the complexation of soluble organic ligands, another appealing to the immobile biopolymers such as the insoluble components of secretion, and the third related to the mechanical forces in association with the direct physical contact between cells and mineral particles.     

Dynamic Analysis of a Single-Column Platform (II)

This paper describes the study of a single-column structure used as well-head platform. In order to check the reliability of computation theory and programme, model tests have been carried out. The paper introduces the conclusion of tests and the dynamic properties of single-column platform are obtained.     

维护体系良性运行 适应新版质量标准

本文介绍了海洋技术研究所质量管理处在维护质量体系运行方面的主要作法和经验体会，结合2000版ISO9201标准的新内容，提出适应新版标准的想法。     

氮与磷的增加对海水无机碳体系影响的实验模拟研究

海洋碳循环是全球变化研究中的重要领域,它不仅在很大程度上决定了全球气温乃至全球气候的变化趋势,而且还是海洋生态系持续发展的基础,它决定着了海洋生态环境变化的走向。众所周知，碳(C)在海洋中以无机态和有机态的形式存在,在海气系统中,大于98%的C以溶解无机碳(DIC)形式存在于水体中(Zeebe et al,2001)其对海洋碳循环的影响至关重要。 氮(N)和磷(P)等营养盐对维持海洋生态系的正常运转起着至关重要的作用。但是，由于近年来人类生产、生活污水的排放以及滩涂和沿岸水域养殖区的长期施肥,它们也作为近岸海区的主要污染物而导致近海海洋生态环境的日益恶化,影响并改变了一些海域的生态结构。如在胶州湾,由于营养盐浓度及结构发生了变化导致该湾地区浮游植物数量和优势种组成的变化(沈志良,2002)近年来,在海洋沿岸带的河口、海湾等水体较浅的透光层内,以孔石莼等绿藻为主要代表的大型海藻开始泛滥,形成大型海藻的水华。海洋生态环境的改变,必然将导致海洋碳循环的变化,从长时间尺度来看也会影响到全球的气候和气温变化。目前,对营养盐与水生藻类之间的响应关系研究已有大量的报道(王勇等,2002；刘媛等，2004；张文俊等，2004)但对于营养盐与海水无机碳体系之间的耦合作用报道甚少。在海洋环境中、C N P作为主要的生源要素,其变化相互影响,并与海水中所存有的海洋生物密切相关。探讨海水中C-N-P的相互耦合关系对于研究海洋生态环境演变过程及效应,阐明海洋碳循环过程的深层次机理,揭示在过量N P作用富营养化条件下,C的迁移转化行为有重要的科学意义和实际价值。本文作者初步研究了模拟条件下C-N-P的相互关系。     

A reliable and sensitive closing gear

The model QQC_(2-2) plankton net closing gear is a device designed to control the stratifiedsampling of the plankton net by means of a messenger. The device in its fie1d use has met with98% success in 766 times of strtified sampling of plankton at 157 stations during separatesurveys of Fujian coast zone, the Taiwan Strait, and the central section of the South China Sea,     

Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins

The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows： Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control （group） had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations （Ca^2＋, Mg^2＋, Na^＋, Mn^2＋, Al^3＋, Fe^3＋, K^＋） were measured by ICP-OES, and the contents of water soluble phosphorus （Pws） and silicon （Siws） were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO3. The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis （2-DE）. The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the     

云南东川因民铜矿金矿化特征及成因初探

因民铜矿发育两种金矿化类型:一是铜矿的伴生金;二是热液构造蚀变岩型。化探样品分析结果显示,金矿化主要赋存于金箔箐断层上、下盘的角砾状蚀变岩内,空间上受岩性和断裂构造的双重控制。邻区拖布卡金矿的成矿地质背景与本区相似,在成矿地质年代上作以类比推测,认为喜马拉雅期是主成矿期。另外通过对光片镜下和化探原生晕样聚类分析研究,发现构造蚀变岩型金矿化具明显的多期多阶段性及热液特征。     

基于道路特征的海量GPS监控数据压缩方法

结合车辆的运动轨迹只能被限制于道路网络上的事实约束,提出了一种基于道路特征的车辆监控数据存储管理方法,存储车辆在道路网特征点处的信息,通过将原始数据中存储的二维地理坐标转换为存储一维的沿道路网的线性参照坐标实现了监控数据和道路网的结合,可极大地节省GPS车辆跟踪数据的存储空间,有利于对车辆数据的进一步分析利用和交通信息的提取.     

乾安地区盐碱地显热通量的测量

文中给出了用大孔径闪烁仪在 2 0 0 0年生长季观测到的盐碱地区显热通量的主要结果 ,并初步计算了当地的水热平衡状况。结果表明 :乾安盐碱地区显热通量占净辐射量的百分比在干旱、非生长季达到 6 5 % ,在多雨、植被生长季仅为 11% ;显热通量因降水而明显降低 ,幅度与降水强度有关 ,反映了当地的气候和土壤特征。文中还把LAS的测量结果与传统的梯度法作了比较 ,结果基本一致。     

陷落柱的探查与综合治理实践

介绍了桃园煤矿井下所见陷落柱的出露特征。为指导巷道施工，消除水患威胁，采取了综合勘探方法查明陷落柱的范围、高度、导水性等情况，并阐述了注浆加固综合治理陷落柱的方法。