珠江三角洲地质灾害的模糊综合评价

珠江三角洲地质灾害种类的多样性与其所处的复杂地质环境和多变的气候条件密切相关。文章选用了地震活动、活动断裂、地壳升降运动、软土地基、地面塌陷以及冲、淤积等６个主要因素作为评价指标，并用模糊数学方法对珠江三角洲内１７个小区作了计算，结果表明该区大部分地区属于轻灾害区，只有滨海沿岸地区属于较重灾害区。     

数字测图技术在阜新市地理数据库更新中的应用

采用GPS和全站仪联合作业的数字测图方式进行了外业快速数据采集。并利用南方CASS软件对数据进行内业成图工作,最后利用大型的地理信息系统软件ArcGIS进行地理数据的入库,从而实现了阜新市区地理数据库的更新,此方式具有速度快、效率高和精度高等优点,可以借鉴到同类的地区。     

武汉东湖不同营养型子湖的水生生物与水域功能

于１９９１－１９９３年研究了武汉东湖４个营养型不同的子湖中水生生物与水域功能,表明各子湖之间各类水生生物的生态特征存在明显差异,茶港湾的ＨＢＣ和ＦＣ的数量,藻类的细胞密度和初级生产量,浮游动物和底栖动物的个体密度等均居４个子湖之首,其水质已达到了超富营养水平,将该湖区的作为污水天然净化区进行管理,可望对东湖的主体湖区起到缓冲和保护作用,郭郑湖的水质状况仅次于茶港湾湖区,处于富营养阶段,该湖区多项生     

布尔莎模型在GPS时间序列融合中的应用

利用“中国地壳运动观测网络”基准网与全球IGS站的公共站点,采用布尔莎模型求取坐标转换7参数,将地壳观测网络工程基准站点位时间序列与全球解进行绑定.结果显示,转换得到的时间序列N、E向中误差约为2、3mm,与直接融合得到的时间序列的单天误差水平相当,转换得到的站间大地线误差大部分在6mm内,表明该转换方法是可行的.     

洞庭湖中枯水期水情变化及其驱动因素

为揭示洞庭湖中枯水期水情变化特征及其驱动因素,采用长短期记忆神经网络模拟洞庭湖出湖流量及湖区水位,通过情境模拟开展水情变化归因分析。洞庭湖1992—2019年9—10月出湖流量大幅减少,主要受长江流量降低的影响。洞庭湖中枯水期水位主要呈下降趋势,其中9—10月平均水位在西洞庭湖、南洞庭湖降幅约1 m,在东洞庭湖降幅约2 m。地形变化对中枯水期水位主要起拉低作用,长江和流域四水流量变化在9—10月起拉低作用、在12月至次年3月起抬升作用,其中对东洞庭湖水位的影响相对更为显著。研究结果可为洞庭湖中枯水期水资源管理和湿地保护提供参考。     

Localization and dynamic expression of a 27.8 kDa receptor protein for lymphocystis disease virus infection in sea bass (<Emphasis Type="Italic">Lateolabrax japonicus</Emphasis>) tissues

Lymphocystis disease virus (LCDV) infects target cells by attaching to a 27.8 kDa receptor (27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies (MAbs) have been developed. However, the 27.8R existence in tissues of sea bass (Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass.     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

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Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder (Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405), we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen, stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish (Sebastes schlegeli Higendorf), redwing sea robin (Lepidotrigla microptera Günther) and turbot (Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method.