Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.     

Preliminary studies On the colonial form of Cylindrotheca sp. （Bacillariophyceae）

From a stock culture of Cylindrotheca sp., non-motile colony-forming cells were observed and isolated.Colony-forming cells produced both single cells and colony-forming cells in liquid medla.the rate of single cell production varied among clones.     

Carbon isotopic fractionation in lipids from methanotrophic bacteria: relevance for interpretation of the geochemical record of biomarkers

Experiments with cultured aerobic methane oxidising bacteria confirm that their biomarker lipids will be significantly depleted in 13C compared to the substrate. The methanotrophic bacteria Methylococcus capsulatus and Methylomonas methanica, grown on methane and using the RuMP cycle for carbon assimilation, show maximum 13C fractionation of approximately 30% in the resultant biomass. In M. capsulatus, the maximum fractionation is observed in the earliest part of the exponential growth stage and decreases to approximately 16% as cells approach stationary phase. This change may be associated with a shift from the particulate form to the soluble form of the methane monooxygenase enzyme. Less than maximum fractionation is observed when cells are grown with reduced methane availability. Biomass of M. capsulatus grown on methanol was depleted by 9% compared to the substrate. Additional strong 13C fractionation takes place during polyisoprenoid biosynthesis in methanotrophs. The delta 13C values of individual hopanoid and steroid biomarkers produced by these organisms were as much as l0% more negative than total biomass. In individual cultures, squalene was 13C-enriched by as much as 14% compared to the triterpane skeleton of bacteriohopaneaminopentol. Much of the isotopic dispersion in lipid metabolites could be attributed to shifts in their relative abundances, combined with an overall reduction in fractionation during the growth cycle. In cells grown on methanol, where there was no apparent effect of growth stage on overall fractionation there were still significant isotopic differences between closely related lipids including a 5.3% difference between the hopane and 3 beta-methylhopane skeletons. Hopane and sterane polyisoprenoids were also 13C-depleted compared to fatty acids. These observations have significant implications for the interpretation of specific compound isotopic signatures now being measured for hydrocarbons and other lipids present in sediments and petroleum. In particular, biomarker lipids produced by a single organism do not necessarily have the same carbon isotopic composition.     

中酸性岩浆体系成矿流体及微量元素地球化学特征

从流体成矿作用角度出发，与酸性岩浆体系有关的成矿流体可以分为：酸性岩浆硅酸盐熔融体，岩浆一热液过渡阶段硅酸盐熔融体及其分异的流体，酸性岩浆熔体分异形成的热水成矿溶液。酸性岩浆体系主要提供热源和部分矿质，其提供的热源驱动地下水淋滤、萃取围岩中的成矿物质形成地下水热液成矿流体。变质岩混合岩化形成花岗质岩浆过程中所形成的混合岩化成矿流体。在此基础上，讨论了上述不同成矿流体的微量元素地球化学特征及其对成矿的控制作用。     

哈萨克斯坦地震预报的生物学新成就(英)

对哈萨克斯坦几次M≥6强震前动物行为异常的有关资料进行了研究，发现动物（20种）是通过其行为反应的变化对地震作出响应的。另外，在阿拉木图的山前地带（地震活动值大于9），对动物血液学和生物学指数上的动态变化进行了观察。     

天然气资源评价重点参数研究

天然气资源评价重点参数主要包括产气率、运聚系数、区带评价参数与可采系数等4项.首先,通过生物气模拟、不同类型干酪根的产气率模拟实验,建立生物气、煤岩、不同类型泥岩与碳酸盐岩的产气率图版;其次,通过相关分析,建立运聚系数与地质参数的关系模型: 运聚系数＝0.298-0.00259×烃源岩年龄 0.218×有机碳含量-0.00223×成藏关键时刻-0.00 236×盖层厚度 0.0009×盖层埋深-0.286×不整合数 0.000104×储层年龄;第三,在大量统计分析的基础上,建立中国天然气区带地质评价参数体系与取值标准;最后,基于岩性和驱动类型两大因素将天然气藏分为5种类型,其中:碎屑岩水驱气藏的可采系数为50%～70%, 碎屑岩气驱气藏75%～90%,碳酸盐岩水驱气藏55%～80%,碳酸盐岩气驱气藏80%～95%,致密气藏40%～55%.     

不同地质条件下各种类型气源岩气态烃产率的求取

气源岩的气态烃产率是评价气源岩生气潜力的重要指标,也是盆地资源评价中的重要参数. 但在不同的地质条件下,同一种气源岩气态烃产率不同.需要有一种新方法求取气源岩的产气率.采用实验与数值模拟相结合的新方法,求取了不同类型气源岩在不同地质条件下的气态烃产率,为我国天然气资源评价提供了重要的地质参数.     

Diagnosis of trace metal contamination in sediments: the example of Ensenada and El Sauzal, two harbors in Baja California, Mexico

Total metal concentrations in sediments from within Ensenada and El Sauzal Harbors are generally higher than at the mouths. Grain-size analyses suggested that this enrichment could be due to the presence of fine-grained sediments in the inner part of the harbors rather than to anthropogenic perturbations. The (Me/Al)(sample) ratios for Pb, Co, Ni and Fe were significantly higher for Ensenada Harbor relative to El Sauzal Harbor, whereas the ratios for Cd, Mn, Zn and Cu were statistically equivalent for both harbors. Calculated enrichment factors [EF(Me)=(Me/Al)(sample)/(Me/Al)(shale)] indicated that the metals showing slight enrichment were those associated with anthropogenic contamination (Pb, Zn), or probably related to primary productivity in the water column (Cd, Co). The levels of most of the metals were not greatly enriched, a consideration that is of the utmost importance when contamination issues are at stake.     

High resolution micromill sampling for analysis of fish otoliths by ICP-MS: effects of sampling and specimen preparation on trace element fingerprints

Otoliths are calcified structures in the head of fish that record environmental information about fish's life history. Gathering the elemental information from the core of an otolith corresponding to the juvenile period of fish's life is critical to discriminate the adult fish to their natal habitats reliably. A high resolution micromill has been used to isolate the otolith core from a whole otolith for elemental analysis. The effects of micromilling procedures (e.g., sectioning, embedding and drilling) on contamination to otolith trace element levels were examined using paired blackfin tuna (Thunnus atlanticus) otoliths. Otoliths were decontaminated by dilute hydrogen peroxide and nitric acid throughout to remove surface contamination. A preconcentration procedure was used to determine the trace elements from the small core material by ICP-MS. It was found that micromilling procedures introduce significant contamination to otoliths, especially for Al, Cu, Pb and Zn. The sectioning procedure caused significant contamination for Co and Cu, while the embedding procedure resulted in contamination for nearly all trace elements (Al, Cd, Co, Cu, Ga, Mn, Ni, Pb, V and Zn). The combined sectioning, embedding and drilling procedure also resulted in contamination for most trace elements. Despite the contamination across all procedural steps, the decontamination procedure effectively removed the surface contamination with the exception of Pb and Zn. Bias (e.g., residual contamination) on Pb was small in comparison to overall concentration of Pb expected to occur in fish otoliths, therefore, its effect may be minor in discriminating individuals. Bias on Zn was larger that could limit its application in discriminating individuals.     

基于EIGEN-6C4的区域重力异常插值方法

重力异常作为地球重力场的一种基本指标,广泛应用于大地测量、地球物理、地质、地震与海洋领域。为了快速获取高精度的未知点重力异常,本文基于EIGEN-6C4重力场模型解算某110 km×110 km区域内重力异常,构建5′×5′的重力异常格网模型,结合格网节点数据采用6种不同的插值算法拟合未知点的重力异常,最后使用外部检核的方法评价插值算法的精度。实验结果表明,6种插值算法的拟合精度均达到4 mgal以内,自然三次样条法和反距离加权插值法拟合精度最高,得到精度达到2.7 magl的重力异常。