Total Synthesis of Marine Cyclic Enol-Phosphotriester Salinipostin Compounds

Due to their structural diversity and variety of biological activities, marine natural products have been the subject of extensive study. These compounds, especially phospholipid polycyclic aromatic hydrocarbons, have a wide range of pharmacological applications, including embedded DNA and central nervous system, anti-tumor, anti-virus, anti-parasite, anti-bacterial, and antithrombotic effects. Unfortunately, the insufficient drug sources have limited the development of these compounds. In this study, we isolated salinpostin compounds from a fermentation solution of marine-derived Salinospora sp., which has a common bicyclic enol-phosphotriester core framework, as well as potent and selective antimalarial activities against P. falciparum with EC_(50)=50 nmolL~(-1). The chemical synthesis of these compounds in greater quantities is necessary for their use in bioactivity studies. Thus we explored a short route with high yields and mild reaction conditions, which can generate combinatorial libraries for drug discovery and lead optimization. We developed a new total synthesis method for six cyclic enol-phosphotriester salinipotin compounds and their diastereomers. For the total synthesis of cyclipostin P, we prepared cyclic enol-phosphotriester salinipostin compounds in 10 steps from a readily accessible starting material, 1,3-dihydroxyacetone, and obtained an overall yield of 1.29%. We fully characterized these compounds by proton nuclear magnetic resonance(~1H-NMR), carbon-13 NMR(~(13)C-NMR), and high-resolution mass spectrometry(HRMS) analyses, and found they coincide absolutely with the same compounds reported previously.     

Experiment Study and Interpretation on Relation between Modulus of Rock and Strain Amplitude

INTRODUCTIONThe modulus of rock is one of the basic elastic parameters of the earth’s medium. Under theassumption of linear elastic theory and Poisson medium,other parameters can be calculated fromit .But the real earth mediumis nonlinear elastic and has not a simple linear relationship between stressand strain.This causes relatively big differences betweenthe dynamic andthe static modulus of rock.In nonlinear elastic theory,the Young’s modulusEis replaced bythe nonlinear elastic modul…     

柴达木盆地东部尕海短钻岩芯记录的过去近400 a区域环境变化

干旱区湖泊沉积可以有效记录区域湿度变化及粉尘活动历史,位于柴达木盆地德令哈南部的咸水封闭湖泊尕海具有恢复区域环境变化的潜力.该地区的长序列环境演变研究已开展了较多的工作,但缺乏短尺度、高分辨率的近代以来的环境变化湖泊记录.通过对尕海深水区短钻岩芯放射性核素210Pb和137Cs的年代测定和沉积物各指标分析,认为尕海沉积物碳酸盐含量可以反映区域湿度变化;而粗颗粒组分(66.9μm)主要由风力搬运入湖,其含量可以指示区域粉尘活动历史.区域近400 a的环境变化可划分为3个阶段:1633 1750 AD:各指标出现大幅度波动,表现出该时期气候环境的不稳定和干湿交替,其中1650 1720 AD的变化波动尤为显著,这与太阳活动进入Maunder极小期存在一定的联系.1750 1950 AD:区域气候环境无明显波动,碳酸盐含量逐渐降低,湿度增加.1950 2010 AD:各指标变动剧烈,区域虽降水增多,但由于蒸发加强,湿度降低;1974 AD以后粗颗粒组分含量急剧上升,表现出高强度尘暴事件.     

高盐强酸性地下水中复合苯系污染物原位芬顿氧化实验研究

在原位氧化过程中,实际场地地下水和含水介质的物化特征是影响氧化效果的重要因素,而目前对此影响的研究较少。以某场地实际高盐强酸性复合苯系污染地下水为研究对象,以地下水中2-硝基-4-甲氧基苯胺(2-nitro-4-methoxyaniline,2-N)和3-硝基-4-甲氧基苯胺(3-nitro-4-methoxyaniline,3-N)为特征污染物,探究芬顿(Fenton)试剂原位氧化特征,并研究液相环境因素(初始H2O2浓度、初始Fe2+浓度、初始pH值、初始醋酸(Acetic acid,HAc)浓度、初始SO4^2-浓度)以及含水层介质对Fenton法去除2-N和3-N的影响。结果显示:(1)Fenton法去除2-N和3-N效果显著,且在初始液相条件为c(H2O2)=7 mmol/L、c(Fe²⁺)=4 mmol/L、pH=4、c(HAc)=0 mg/L和c(SO4^2-)=0 mmol/L时去除效果最佳;(2)各因素对Fenton法氧化2-N和3-N的影响不同,加入H₂O₂和Fe²⁺使2-N和3-N去除率上升,增大HAc浓度使2-N和3-N去除率下降;(3)含水层介质对2-N和3-N具有一定吸附性,且对3-N的吸附性强于2-N,二者在本实验中最大吸附态占比分别为29%和42%,而吸附态的存在会抑制Fenton法对2-N和3-N的去除;(4)矿物分析结果显示介质含有少量黄铁矿,在硫酸环境下,介质腐蚀溶解释放Fe²⁺,在达到一定浓度后,无需额外添加Fe²⁺即可完成Fenton反应进而去除2-N和3-N。     

新疆侏罗纪古构造及其对聚煤盆地的控制

古构造是控制聚煤盆地形成与演化的首要地质因素。新疆赋煤区是由前寒武纪形成的大陆古板块、微型陆块与古生代形成的造山带镶嵌而成的复合大陆块体。早-中侏罗世,区域挤压应力松弛甚至出现区域拉张应力,盆-山耦合型前陆-克拉通盆地成为新疆早-中侏罗世聚煤盆地的主体;基底性质不同的两个构造单元之间及造山带内部,形成带状拉张断陷;在某些早期弧后盆地和残留海盆分布的地区,发生继承性沉降,成为陆相聚煤盆地。在具有前寒武纪基底的前陆-克拉通盆地和继承性盆地中,聚煤作用最为强盛;发育在古生代造山带之上的伸展断陷型山间盆地,聚煤强度的横向变化较大。     

Establishment of a spleen cell line from large yellow croaker Pseudosciaena crocea and its primitive application in foreign gene transfection

A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco’s modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies.     

延庆平原区水文地质条件研究

近年来,随着世园会、冬奥会、延庆新城、中关村延庆园等的规划建设[1],延庆平原区的工程建设项目越来越多。延庆平原区在第四纪整个演化过程中[2],比北京平原上升快、反复大,地质沉积规律复杂,且研究区内主要河流(古八达岭河和古妫水河)曾发生过流向倒转,造成研究区内地形地貌及地下水条件复杂,水文地质条件差异较大,和临近的北京平原区地下水分布规律有所不同,复杂的水文地质条件对该地区工程建设的影响较大。通过查阅调查区相关工程地质与水文地质背景资料,同时结合在延庆平原区的地下水监测信息,对延庆平原区的水文地质条件进行了调查研究,并根据调查结果对该地区进行了水文地质条件分区,以指导相关工程建设。     

Application of rock creep experiment in calculating the viscoelastic parameters of earth medium

The viscosity of earth medium can explain many geophysical phenomena. The uplifting velocity of Ti- betan Plateau may be caused by extrusion of the low viscosity channel in middle and lower crust[1]. The post-deformation after earthquake also relates to the rheology of earth medium[2]. The aftershock sequence is considered a process of strain release after stress dropped[3]. There are two kinds of models in the study of rheological character of crustal medium, elastoplas- only when the forced…     

基于MVC架构新媒体公众气象服务技术研究

基于MVC框架的新媒体平台开发,已经成为现代J2EE开发中一种常用模式。为深入开展气象新媒体公众服务产品研发和平台应用,通过“模型视图控制器”设计典范,设计支撑新媒体公众气象服务的MVC架构,实现气象服务产品从采集到加工处理再到终端显示等功能,使其具备简约化、规范化、开放性、高效性和可扩展性等优点,便于与外围系统实现数据的交互、共享和内部功能模块间的数据交互。通过基于MVC架构新媒体公众气象服务技术研究,为气象新媒体平台提供拓展接口和技术支撑,使其可与水文、环境、农业、交通等多部门数据共享,利用这些部门的监测数据,结合气象观测数据、GIS数据等,实现气象资料自动采集分析、智能产品制作分发、精细化预报服务、实况监测预警、灾害天气分析处理等功能,为公众提供精细化气象服务、气象为农服务、水情信息服务、地质灾害预警、灾害天气预报预警等多种气象服务产品。