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Immunological and biochemical techniques were used to investigate organic matter in microbial mats from Solar Lake (Sinai, Egypt). Antibodies, elicited in rabbits against samples taken at different depths from a core of these mats, were used for the detection of immunological determinants preserved in these samples. Special attention was given to an antiserum directed against a top mat fraction (3–10 mm depth) and an antiserum against a fraction from a lower section of the core (380–390 mm depth). Both antisera, tested in a dot-blot immunobinding assay, were found to give positive reactions with organic matter from different depths of the sediment. An immunohistochemical experiment with the antisera points to antigenic determinants on Recent and subfossil sheaths of Microcoleus. In addition, extracts of sediment samples were submitted to electrophoresis in polyacrylamide gels in order to characterize the separated macromolecules by immunological means. The results showed a rapid decrease in the number of antigenic determinants from Recent to subfossil samples. However, even in the deepest layers of the sediment (690–700 mm depth) intact biopolymers were detected. 相似文献
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The dissolution of calcified invertebrate skeletons releases an elaborate mixture of proteins, glycoproteins and polysaccharides. These skeletal matrix macromolecules are thought to play a major role in calcification and were widely used for phylogenetical studies. We tested the reactivity of water-soluble macromolecules from a wide range of invertebrate skeletons with two antisera raised against the shell matrix of the bivalve, Pinna nobilis. Projections of our results on the phylogenetical tree of Starobogatov (1992) show for the first time that, during evolution, antigenic determinants may degenerate in some stocks while they remain intact in others. The phylogenetic implications of these patterns of disjunct degeneration are discussed. 相似文献
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Programmes for the study of global change correctly identify the development of integrated numerical models of earth dynamics as an ultimate goal. But ongoing research and plans for future investigations suffer from a major flaw: they fail to treat life as a global phenomenon. We propose a long-term strategy for the incorporation of the biosphere as a module into general circulation models (Gas). 相似文献
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