Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

Cycle anatomy and variability in the storm-dominated type cincinnatian (Upper Ordovician): coming to grips with cycle delineation and genesis

Although parasequence and sequence are scale-independent terms, they are frequently applied only to specific scales of cycles. For example, meter-scale cycles are commonly assumed to be parasequences or PACs. In the Upper Ordovician Kope and Fairview Formations of northern Kentucky, we examined a succession of 50 meter-scale cycles that have been variously interpreted as deepening-upward, shallowing-upward, or showing no relationship with water depth. Our analysis shows that these cycles, characterized by shifts in storm-bed proximality, are highly variable in their thickness and internal construction. Most cycles are best considered high-frequency sequences, because deepening-upward intervals are common, and many cycles contain evidence of abrupt basinward shifts in facies as expected at sequence boundaries. A minority fit the parasequence model of shallowing-upward cycles bounded by flooding surfaces. Larger, 20 m scale cycles are defined by systematic thickening and thinning trends of meter-scale cycles. However, meter-scale cycles do not display any systematic trends in cycle anatomy as a function of position within the 20 m cycles or position within the Kope and Fairview Formations. The high cycle variability and the lack of systematic stratigraphic organization with respect to longer-term cyclicity reflect either the irregularity of relative sea-level changes, the poor recording of sea-level changes in this deep-water setting, or the generation of these cycles by climate-induced cyclicity in storm intensity. These three mechanisms would generate similar patterns at the outcrop scale, so it is not possible at the present to distinguish between them.     

集体土地不动产确权登记试点工作中重点问题剖析

结合古蔺县农村土地范围内不动产确权登记入库项目,简述了项目概况和基本原则,详述了在集体土地不动产登记确权试点中实际出现的一些核心问题并提出切实可行的解决办法。     

湖南仁里稀有金属矿田206号锂辉石伟晶岩脉地球化学特征及成矿时代

湖南仁里稀有金属矿田是中国近年来新发现的一处重要的花岗伟晶岩型铌、钽、锂等稀有金属矿产地,文章针对矿田含锂伟晶岩地球化学特征、成矿时代及其与花岗岩的关系,选取传梓源锂铌钽矿床内规模最大的206号锂辉石伟晶岩脉开展地球化学和白云母Ar-Ar定年工作,并与区内其他伟晶岩、花岗岩的地球化学特征、成岩时代对比分析.传梓源206号锂辉石伟晶岩属高分异稀有金属伟晶岩,形成时代为(135.4±1.4)Ma,岩石地球化学表现为高硅、高铝、低钙、相对富碱、钙碱性及过铝质特征;稀土元素总量很低,以轻稀土元素为主;微量元素富集Cs、Rb、U、Ta、Nb、Zr、Hf,相对亏损Ba、Ti,Zr/Hf、Nb/Ta比值低且集中.幕阜山地区稀有金属成矿可分为2期:第1期稀有金属成矿时代约145 Ma,与燕山早期岩浆活动有关;第2期稀有金属成矿时代135～125 Ma,为主成矿期,该期稀有金属伟晶岩与燕山晚期的二云母二长花岗岩存在成因联系,两者为同源岩浆连续结晶分异过程中不同阶段的产物.稀有金属富集成矿经历了岩浆-热液两阶段作用,Be、Nb、Ta、Li、Rb、Cs等稀有元素的富集多发生于岩浆结晶分异晚期,热液作用使Ta、Li、Rb、Cs再次富集.     

湖南仁里稀有金属矿田36号伟晶岩地球化学特征、成矿时代及其意义

位于幕阜山岩体西南缘的仁里稀有金属矿田是近年来华南地区新发现的一处重要花岗伟晶岩型稀有金属矿产地,铌钽矿体赋存于岩体内外接触带伟晶岩中,具有明显成矿分带性.文章选取仁里矿床岩体内接触带36号伟晶岩脉为研究对象,开展伟晶岩的地球化学及云母Ar-Ar年代学研究,探讨其与花岗岩围岩成因关系及岩体内接触带伟晶岩的成岩成矿时代,以丰富仁里矿田稀有金属成矿作用研究.研究表明,36号伟晶岩具高硅(w(SiO2)为72.87％～76.21％)、高铝(w(Al2O3)为13.69％～15.14％)、低钙镁铁、相对富碱(w(Na2O+K2O)为6.59％～8.33％)、(高钾)钙碱性及过铝质特征;微量元素总体富集Nb、Ta、Hf、U等高场强元素(HFSE),相对亏损Ba、Sr等离子亲石元素(LILE);稀土元素总量(ΣREE)13.95×10-6～71.63×10-6,轻稀土元素富集,重稀土元素亏损,具壳源特征.白云母Ar-Ar坪年龄为早白垩世(136.6±1.4)Ma,成矿作用发生于仁里矿田稀有金属大规模成矿的早阶段.地球化学及矿物学特征显示,36号伟晶岩与花岗岩围岩具有良好的演化关系,结合野外观察现象及成岩年龄,认为36号伟晶岩为花岗质岩浆侵位后残余岩浆结晶分异的产物,母岩为中粗粒片麻状黑云母二长花岗岩.     

CSAMT与AMT数据“拼接”处理——以湖南仁里铌钽矿床7号剖面为例

音频大地电磁测深(AMT)与可控源音频大地电磁测深(CSAMT)均为深部勘查常用方法。AMT方法采集频带较宽,但存在“死频带”,在“死频带”范围无法完全得到地质体响应。CSAMT信噪比高,但通常受发射功率的限制,收发距离一般放置不够远,容易过早进入“近区”。本文展示了将两种方法数据拼接组合的一种处理方式:首先在前人理论研究的基础上,利用CSAMT和AMT均匀半空间模型进行了正演计算,结果表明,CSAMT远区与AMT数据拼接具有可行性;选择湖南仁里铌钽矿床7号剖面的野外实测数据进行实验,将同测点位置的CSAMT远区数据和AMT同测点同模式数据相“拼接”,合成新AMT数据;最后进行反演,反演结果与钻探结果对应较好,表明该方法具有一定的效果。     

一种基于微服务架构的突发事件预警辅助决策系统的设计与实现

单体式架构应用在突发事件预警辅助决策系统开发中不能满足现实需求,本文引入了微服务架构设计开发该辅助决策系统.通过分析微服务架构在复杂系统中相对于传统单体式架构的应用优势,设计出一种基于微服务架构的突发事件预警信息发布辅助决策系统,该系统选用Spring Cloud微服务框架,并对其进行适当的扩展,创建了基于该系统设计的注册中心与网关.系统采用二三维一体化地理信息系统作为展示平台,通过接入各行业静态、危险源动态监测数据,根据设定的模型进行数据融合、处理,辅助进行预警信息生成、发布及应急处置阶段的指挥决策.所设计方案在湖北省突发事件预警发布辅助决策系统中得以实际应用,验证了该类系统使用微服务架构的合理性和有效性.     

兰州市主城区房价分异及驱动因素研究

以2021年兰州市主城区678个居住小区房价数据为基础,引入地理场模型量化影响房价的外部因素,通过空间自相关分析、多尺度地理加权回归等模型对房价分异的空间格局及驱动因素的作用机理、带宽差异展开研究,以期为推动河谷型城市房产市场的公平发展提供参考。结果表明:(1)兰州市主城区平均房价为13739元·m^-2,空间上呈现“一主三副”的带状多中心组团式分布格局,房价由多中心向四周递减,价格相似的小区在地理空间上邻近分布,具有“小集中、大分散”的局部空间特征。(2)房价分异是多种驱动因素共同作用的结果,区位特征中的主商圈对房价的影响居于首位,建筑特征中的房龄、容积率和邻里特征中的中学数量、绿化率等对房价的影响较大,城市地理特征对房价具有显著影响,愈靠近黄河的小区、房价越高。(3)各驱动因素的带宽差异明显,主商圈、医院等小尺度变量存在高度空间异质性,而容积率、黄河等全局变量基本不存在空间异质性。