Assessing degradation capability of aerobic indigenous microflora in PAH-contaminated brackish sediments

A study was conducted to determine polycyclic aromatic hydrocarbons (PAHs) distribution and microbial population changes in brackish sediments from an Italian lagoon included in the Ramsar List of Wetlands of International Importance. The presence and level of PAH-degrading bacteria were estimated by the most probable number (MPN) enumeration technique, whereas degradation capability towards target compounds was checked against loss of spiked PAHs (Phenanthrene, Anthracene and Fluoranthene) in MPN tubes after incubation in optimal conditions. Chemical analyses and microbiological counts suggested a potential for PAHs biodegradation by natural occurring populations of sediment microorganisms, thus indicating an "optimal range" in sediment PAHs concentrations, outside of which the natural selection of the indigenous microflora did not occur. The MPN procedure here described, provided an effective and reliable way to simultaneously determine microbial population densities and subsequent confirmation of the biodegradation capability of sediment indigenous microflora when exposed to laboratory and environmental concentrations of PAHs.     

Microhabitat preferences and depth distribution of combtooth blennies (Blenniidae) in the Gulf of Trieste (North Adriatic Sea)

The microhabitat preferences and depth distribution of blenniid species (Blenniidae) in the Gulf of Trieste (North Adriatic Sea) were surveyed using the all‐occurrence sampling method, a non‐destructive visual census method, aided by SCUBA diving. Fourteen species were identified during the entire survey. Four species showed to be indiscriminate in their microhabitat choice while 10 species were classified as infrequent in the surveyed coastal area. A statistically significant correlation was found between the blenniid assemblage and nine microhabitat variables. The blenniid assemblage was divided in two main groups, by the use of canonical correspondence analysis, electivity index, and the depth distribution analysis. The first group comprises species that dwell in surface waters and show a high positive correlation with boulders, the presence of Mytilus galloprovincialis, cirripeds and empty holes bored by Lithophaga lithophaga. The second group includes species that mostly inhabit deeper waters and show a high positive correlation with rocks covered by precoralligenous bioformations.     

Sources and biogeochemical cycling of particulate selenium in the San Francisco Bay estuary

As part of a study of estuarine selenium cycling, we measured the concentration, chemical form (speciation), and distribution of particulate selenium under various river flow conditions in the North San Francisco Bay (from the Golden Gate to the Sacramento and San Joaquin Rivers). We also conducted laboratory studies on the accumulation of selenium by phytoplankton, the critical first step in the transformation of dissolved to particulate selenium. Total particulate selenium concentration in the North SF Bay was relatively constant between high and low flow periods, ranging spatially from 0.05 to 0.35 nmol l⁻¹ and comprising between 5 and 12% of the total water column selenium inventory. Mean concentrations were generally highest in the Carquinez Strait–Suisun Bay region (salinity 0–17) and lowest in Central Bay. However, selenium content of suspended particles varied with river flow, with higher content during low flow (9.76 ± 4.17 nmol g⁻¹; mean ± sd; n = 67) compared to high flow (7.10 ± 4.24 nmol g⁻¹; n = 39). Speciation analyses showed that most particulate selenium is organic selenide (45 ± 27%), with a smaller proportion (typically <30%) of adsorbed selenite + selenate and a varying proportion (35 ± 28%) of elemental selenium. Based on the amount of elemental selenium in the seston (total suspended material), we calculate that resuspension of estuarine sediments could contribute 29–100% of particulate selenium in the water column. While selenium content of SF Bay seston (>0.4 μm) is relatively unenriched compared to phytoplankton (13.6–155 nmol g⁻¹ dry weight) on a mass basis, when normalized to carbon or nitrogen, seston contains a similar selenium concentration to SF Bay sediments or phytoplankton cultures. SF Bay seston is thus comprised of selenium-rich phytoplankton and phyto-detritus, but also inorganic clay mineral particles that effectively “dilute” total particulate selenium. Selenium concentrations in algal cultures (11 species) exposed to 90 nmol l⁻¹ selenite show relatively large differences in selenium accumulation, with the diatoms, chlorophytes and cryptophytes generally having lower selenium cell content (3.8 ± 2.7 × 10⁻⁹ nmol selenium cell⁻¹) compared to the dinoflagellates (193 ± 73 × 10⁻⁹ nmol selenium cell⁻¹). Because phytoplankton are such a rich (but variable) source of selenium, their dynamics could have a profound effect on the particulate selenium inventory in the North SF Bay.     

Argon isotope fractionation induced by stepwise heating

Noble gas isotopes are widely used to elucidate the history of the rocks in which they have been trapped, either from distinct reservoirs or by accumulation following radioactive decay. To extract noble gases from their host rocks, stepwise heating is the most commonly used technique to deconvolve isotopically different components, e.g., atmospheric, in situ radiogenic, or excess radiogenic from mantle or crustal reservoirs. The accurate determination of the isotopic composition of these different components is of crucial importance, e.g., for ages obtained by ⁴⁰Ar-³⁹Ar stepheating plateaus. However, diffusion theory-based model calculations predict that the stepwise thermal extraction process from mineral phases induces isotope fractionation and, hence, adulterates the original composition. Such effects are largely unconsidered, as they are small and a compelling experimental observation is lacking. We report the first unequivocal evidence for significant mass fractionation of argon isotopes during thermal extraction, observed on shungite, a carbon-rich Precambrian sedimentary rock. The degree of fractionation, as monitored by ³⁸Ar/³⁶Ar and ⁴⁰Ar/³⁶Ar ratios, very well agrees with theoretical predictions assuming an inverse square root dependence of diffusion coefficient and atomic mass, resulting in easier extraction of lighter isotopes. Hence, subatmospheric ⁴⁰Ar/³⁶Ar ratios obtained for argon extracted at low temperatures may not represent paleoatmospheric argon. Shungite argon resembles modern atmospheric composition, but constraints on the timing of trapping appear difficult to obtain, as shungites are multicomponent systems.In ⁴⁰Ar-³⁹Ar stepwise heating, the isotope fractionation effect could cause systematic underestimations of plateau ages, between 0.15 and 0.4% depending on age, or considerably higher if samples contain appreciable atmospheric Ar. The magnitude of this effect is similar to the presently achieved uncertainties of this increasingly precise dating technique. Our results also indicate the importance of thermally activated diffusion as a possible fractionation mechanism, e.g., for hydrothermal gas exhalations, or for carbonaceous carrier phases such as “Q” in meteorites that have been suggested as carriers of highly fractionated noble gas residues from the early solar nebula.     

Geochronological and isotopic records of crustal storage and assimilation in the Wolverine Creek–Conant Creek system,Heise eruptive centre,Snake River Plain

Understanding the processes of differentiation of the Yellowstone–Snake River Plain (YSRP) rhyolites is typically impeded by the apparent lack of erupted intermediate compositions as well as the complex nature of their shallow interaction with the surrounding crust responsible for their typically low O isotopic ratios. A pair of normal-δ¹⁸O rhyolitic eruptions from the Heise eruptive centre in eastern Idaho, the Wolverine Creek Tuff and the Conant Creek Tuff, represent unique magmatic products of the Yellowstone hotspot preserving abundant vestiges of the intermediate differentiation steps leading to rhyolite generation. We address both shallow and deep processes of magma generation and storage in the two units by combining high-precision ID–TIMS U–Pb zircon geochronology, trace element, O and Hf isotopic studies of zircon, and Sr isotopic analyses of individual high-Mg# pyroxenes inherited from lower- to mid-crustal differentiation stages. The zircon geochronology confirms the derivation of both tuffs from the same rhyolitic magma reservoir erupted at 5.5941 ± 0.0097 Ma, preceded by at least 92 ± 14 ky of continuous or intermittent zircon saturation approximating the length of pre-eruptive magma accumulation in the upper crust. Some low-Mg# pyroxenes enclosing zircons predate the eruption by at least 45 ± 27 ky, illustrating the co-crystallisation of major and accessory phases in the near-liquidus rhyolitic melts of the YSRP over a significant period of time. Coeval zircon crystals are isotopically heterogeneous (two populations at εHf ~?5 and ?13), requiring the assembly of isotopically distinct melt pockets directly prior to, or during, the eruption. The primitive Mg# 60–90 pyroxenes are out of isotopic equilibrium with the host rhyolitic melt (⁸⁷Sr/⁸⁶Sr_i = 0.70889), covering a range of ⁸⁷Sr/⁸⁶Sr_i = 0.70705–0.70883 corresponding to ratios typical of the most radiogenic YSRP basalts to the least radiogenic YSRP rhyolites. Together with the low εHf in zircon, the Sr isotopic ratios illustrate limited assimilation dominated by radiogenic Archean crustal source materials incorporated into variably evolved YSRP melts as they progress towards rhyolitic compositions by assimilation–fractional crystallisation.     

Forecasting rock slope failure: how reliable and effective are warning systems?

Warning systems are increasingly applied to reduce damage caused by different magnitudes of rockslides and rockfalls. In an integrated risk-management approach, the optimal risk mitigation strategy is identified by comparing the achieved effectiveness and cost; estimating the reliability of the warning system is the basis for such considerations. Here, we calculate the reliability and effectiveness of the warning system installed in Preonzo prior to a major rockfall in May 2012. “Reliability” is defined as the ability of the warning system to forecast the hazard event and to prevent damage. To be cost-effective, the warning system should forecast an event with a limited number of false alarms to avoid unnecessary costs for intervention measures. The analysis shows that to be reliable, warning systems should be designed as fail-safe constructions. They should incorporate components with low failure probabilities, high redundancy, have low warning thresholds, and additional control systems. In addition, the experts operating the warning system should have limited risk tolerance. In an additional hypothetical probabilistic analysis, we investigate the effect of the risk attitude of the decision makers and of the number of sensors on the probability of detecting the event and initiating a timely evacuation, as well as on the related intervention cost. The analysis demonstrates that quantitative assessments can support the identification of optimal warning system designs and decision criteria.     

Using Clustered Heat Maps in Mineral Exploration to Visualize Volcanic-Hosted Massive Sulfide Alteration and Mineralization

Natural Resources Research - This study proposes an extension of a visualization approach common in biochemistry (the clustered heat maps—CHMs) to geochemical data with the main objective of...     

Towards new applications of underwater photogrammetry for investigating coral reef morphology and habitat complexity in the Myeik Archipelago,Myanmar

Photogrammetry represents a non-destructive, cost-effective tool for coral reef monitoring, able to integrate traditional remote sensing techniques and support researchers’ work. However, its application to submerged habitats is still in early stage. We present new ways to employ Structure from Motion techniques to infer properties of reef habitats. In particular, we propose the use of Digital Surface Models and Digital Terrain Models for assessing coral colonies extension and height and discriminating between seabed and coral cover. Such information can be coupled with digital rugosity estimates to improve habitat characterization. DTM, DSM and orthophotos were derived and used to compute a series of metrics like coral morphologies, reef topography, coral cover and structural complexity. We show the potentialities offered by underwater photogrammetry and derived products to provide useful basic information for marine habitat mapping, opening the possibility to extend these methods for large-scale assessment and monitoring of coral reefs.     

Biogenic and lithogenic magnetic minerals in Atlantic and Pacific deep sea sediments and their paleomagnetic significance

The carrier of the natural magnetization of deep sea sediments was characterized by mineralogical, electron microscopic, and rock magnetic investigations. Magnetic single domain (SD) and pseudo single domain (PSD) particles which are most important for the stable remanent magnetization were separated from the magnetic »coarse fraction« and concentrated as magnetic »fine fraction«. The magnetic coarse fraction consists of lithogenic magnetite and titanomagnetite, which often contains exsolution-lamellae of ilmenite. Both minerals are partially maghematized and occur isolated in the sediment or embedded in rock particles, in regionally different concentrations. The magnetic fine fraction consists of lithogenic magnetite and titanomagnetite and biogenic magnetite (magnetofossils = fossil bacterial magnetosomes), the latter generally maghematized.A graphical method is described which allows the classification and characterization of the magnetic fine fraction by demagnetization of the anhysteretic remanent magnetization (ARM) of whole sediment samples. Three groups with different magnetic properties can be distinguished, characterized by three ARM type-curves: Type A curves are associated with sediments from abyssal plaines. They show nearly identical ARM properties and are typical for magnetofossils.Type B curves are produced by sediments from the vicinity of volcanic regions. Their shapes are variable to a certain degree and indicate two lithogenic magnetic phases.Type C curves are found for sediments from submarine ridges and regions with input of terrigenous detritus. These curves have the largest deviations among each other indicating a magnetic multi-phase assemblage including magnetofossils.

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Zusammenfassung Die Träger der Magnetisierung von Tiefseesedimenten wurden mineralogisch, elektronenmikroskopisch und gesteinsmagnetisch untersucht. Magnetische Eindomänen (SD) und Pseudo-Eindomänen (PSD) Partikel, die für eine stabile remanente Magnetisierung wichtig sind, wurden als magnetische »Feinfraktion« von der magnetischen »Grobfraktion« abgetrennt. Die magnetische Grobfraktion besteht aus lithogenem Titanomagnetit und Magnetit die teilweise maghemitisiert sind und teilweise auch Ilmenit-Entmischungslamellen aufweisen. In regional unterschiedlichen Konzentrationen liegen sie frei im Sediment oder in silikatischer Matrix eingebettet vor. Die magnetische Feinfraktion besteht sowohl aus lithogenem Titanomagnetit und Magnetit, als auch aus biogenem Magnetit (Magnetofossilien = fossile bakterielle Magnetosomen); letzterer ist größtenteils maghemitisiert.Es wird eine grafische Darstellungsmethode beschrieben, die anhand von Untersuchungen der anhysteretischen remanenten Magnetisierung (ARM) von Sedimentproben eine Charakterisierung der magnetischen Feinfraktion erlaubt. Es lassen sich dadurch drei Gruppen mit unterschiedlichen magnetischen Eigenschaften unterscheiden, die durch drei Gruppen von ARM-Kurventypen charakterisiert sind.Kurventyp A wird bei Sedimenten aus Tiefsee-Ebenen beobachtet. Die ARM-Daten sind nahezu identisch und zeigen ein Verhalten, wie es für Magnetofossilien typisch ist. Kurventyp B tritt bei Sedimenten aus dem Einzugsbereich vulkanischer Gebiete auf. Er zeigt eine größere Variation und die Form der Kurven spricht für ein System aus zwei lithogenen magnetischen Komponenten.Kurventyp C gehört zu Sedimenten aus submarinen Rücken und dem Einzugsgebiet terrigener Schüttungen. Die Kurvenverläufe sind uneinheitlich und sprechen für ein magnetisches Mehrkomponenten-System mit Beteiligung von Magnetofossilien.

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Résumé Les minéraux porteurs du magnétisme dans les sédiments de mer profonde ont été explorés par les méthodes de la minéralogie, de la microscopie électronique et du magnétisme des roches. Les particules qui correspondent à un domaine magnétique unique (SD) et pseudo-unique (PSD), significatives pour un magnétisme rénanent stable, ont été concentrées comme «fraction magnétique fine» après séparation de la «fraction magnétique grossière». Cette dernière consiste en magnétite et titanomagnétite lithogéniques, qui renferment souvent des lamelles d'exsolution d'ilménite. Ces deux minéraux sont partiellement maghémitisés; ils se présentent isolés ou inclus dans des fragments de roches, avec des concentrations régionales diverses. La fraction magnétique fine consiste en magnétite et titanomagnétite lithogéniques, ainsi qu'en magnétite biogénique (magnétofossile = magnétosome fossile bactérien), cette dernière ordinairement maghemitisée.Les auteurs présentent une méthode graphique qui permet de caractériser la fraction magnétique fine à partir de l'examen du magnétisme rémanent anhystérique (ARM) de l'échantillon de sédiment. Cette méthode permet de distinguer trois groupes de propriétés magnétiques différentes, caractérisés par trois types de courbes ARM. Les courbes de type A caractérisent les sédiments de plaines abyssales; elles montrent des propriétés ARM presque identiques et sont typiques pour les magnétofossiles. Les courbes de types B sont fournies par les sédiments voisins des régions volcaniques; leurs formes varient dans une certaine mesure et indiquent un système à deux composants magnétiques lithogéniques. Les courbes de type C correspondent aux sédiments des crêtes sous-marines et des régions à apports terrigènes; ces courbes présentent entre elles des différences plus marquées, ce qui indique un système magnétique à composants multiples, comportant des magnétofossiles.

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