基于MSAP技术的海带DNA甲基化初步研究

DNA甲基化在调节基因表达和控制细胞分化过程中扮演着重要的角色,其作为表观遗传修饰形式之一,已成为后基因组时代的研究热点。本文采用检测基因组CpG位点甲基化水平的MSAP技术(Methylation-Sensitive Amplification Polymorphism),对海带配子体与孢子体世代进行研究,研究发现:孢子体世代甲基化水平(25.64%)显著高于配子体世代(♂5.50%和♀4.48%);低温处理能引起DNA甲基化位点及比率的变化,直接反映在配子体克隆同步发育率的显著提高;对于海带孢子体世代不同组织部位甲基化程度的检测表明,同一株海带不同组织部位的甲基化程度不同(21.79%~29.49%),其中以Ⅳ型为主,同一组织部位在不同个体中甲基化程度也存在差异。研究为进一步从表观遗传学角度探讨海带不同倍型的发育机制和配子体对低温的应答机制奠定基础。     

塔里木陆块在Rodinia超大陆中位置的新认识——来自地层对比和古地磁的制约

塔里木周缘的新元古代地层中均记录了涉及Rodinia聚合和裂解的构造热事件,但塔里木在Rodinia超大陆中位置尚存争议.本文综合地层对比以及古地磁的研究方法,将塔里木陆块在Rodinia超大陆中置于澳洲板块的西北缘,并且塔里木的西南缘(现今位置)和澳洲的西北缘(现今位置)相连.基于塔里木周缘的构造热事件和塔里木、澳洲运动学特征分析,认为塔里木陆块周缘在约800~700 Ma中发生了强烈的裂谷事件,导致塔里木从Rodinia超大陆中裂解,但塔里木并没有完全从澳洲裂离,而是随澳洲一起,加入冈瓦纳大陆.在约450 Ma左右,塔里木与澳洲发生分离,其原因为古特提斯洋的扩张.     

Large-scale physical modeling study on the interaction between rockfall and flexible barrier

Flexible barriers have been widely applied in rockfall mitigation in recent years. However, the behavior of flexible barriers under the impact of boulders is still not fully understood. To investigate the interaction between a flexible barrier and a falling boulder, a large-scale physical modeling device has been constructed at a site in Hong Kong. Using this device, large-scale impact tests using boulders with different diameters were conducted. Test results are presented and analyzed in this paper. The motion of the boulder during impact is traced and analyzed. The impact forces on the flexible ring net and the supporting structures are measured and compared. From the comparison, the impact reduction rates (IRR) of boulders with different diameters are calculated. Moreover, a simple approach for estimating the impact loading of a boulder on a flexible barrier is proposed in this study. This approach is calibrated and verified using measured impact forces in the tests.     

阴离子交换膜生物反应器在不同水力停留时间下还原饮用水中ClO4-的研究

在不同水力停留时间(HRT)下考察了阴离子交换膜生物反应器对饮用水中ClO4-的还原效果.研究结果表明,在进水ClO4-浓度为60 μg/L情况下,HRT从5h逐渐降低到2.5h,水流动室出水ClO4-浓度随着HRT降低呈增大趋势,但运行稳定后出水ClO4-浓度均低于18μg/L.厌氧生物反应室在HRT降低初期高氯酸盐有一定的积累,随着运行时间的增加,积累的ClO4-能被厌氧微生物还原,厌氧污泥混合液中ClO4-浓度低于2.0 μg/L,说明厌氧生物反应室具有较强的抗负荷冲击能力.水流动室出水pH稳定在7左右,电导率和Cl-随着HRT降低而增大.     

Numerical study of retention efficiency of a flexible barrier in mitigating granular flow comparing with large-scale physical modeling test data

Retention behavior of a flexible barrier in mitigating a granular flow is still an open problem not fully understood, especially due to the complexity of the granular material and the flexible barrier. Understanding the retention mechanism and quantifying the influencing factors of retention efficiency are desirable for optimizing the design and minimizing the maintenance cost of a debris-resisting flexible barrier. In this paper, a numerical model, based on the discrete element method, is presented, calibrated, and validated to analyze the interaction between a granular flow and a flexible net. A full-scale numerical simulation is first performed to compare with a large-scale physical modeling test in the literature and validate the applied parameters in the simulation. The interaction and deposition characteristics of the granular flow interacting with a flexible net are revealed. Afterward, parametric study is performed to investigate the effects of the internal friction angle (φ) of debris material and the relative mesh size of flexible net on the retention efficiency and clogging mechanism of a flexible barrier. The simulation results illustrate that the particle passing ratio (P) increases with increment of the friction angle of particles and enlargement of the mesh size of a flexible net. Both parameters have critical effects on the retention efficiency of a flexible barrier in intercepting a granular flow. Therefore, the friction angle and the particle size distribution characteristics of the debris material are suggested being used for optimization of the mesh size and more efficient design of debris-resisting flexible barriers.