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1.
2.
M.A. van Leeuwe L.A. Villerius J. Roggeveld R.J.W. Visser J. Stefels 《Marine Chemistry》2006,102(3-4):267-275
A recent development in algal pigment analysis by high-performance liquid chromatography (HPLC) is the application of automation. An optimization of a complete sampling and analysis protocol applied specifically in automation has not yet been performed. In this paper we show that automation can only be successful if the various methodological aspects of the sampling and analysis protocol are considered in coherence. We introduce an optimized protocol that involves freeze-drying of the sample, subsequent extraction in 90% acetone and the application of water-packing during analysis. The method was evaluated on both natural plankton populations and a broad spectrum of microalgal cultures: Thalassiosira weisflogii (Bacillariophyceae), Emiliania huxleyi (Prymnesiophyceae), Phaeocystis globosa and Phaeocystis antarctica (Prymnesiophyceae) and Pyramimonas sp. (Prasinophyceae). Whereas pigment extracts were unstable in methanol, with recorded chlorophyll a losses from 10% to 60% per day, pigment degradation rates in acetone were generally less than 1% over 18 h storage in the autosampler (4 °C). In addition, it was found that the extraction efficiency of acetone significantly increased upon freeze-drying prior to extraction. Increases as high as 50–60% were measured in P. antarctica. The application of water-packing of the sample during injection resulted in improved peak shape and peak separation, without diluting the pigment concentrations. Automation is especially beneficial for application in the field, when mixed algal assemblages and low biomass put a high demand on the sensitivity as well as reproducibility of the method. 相似文献
3.
4.
It is becoming more apparent that commonly used statistical methods (e.g. analysis of variance and regression) are not the
best methods for estimating limiting relationships or stressor effects. A major challenge of estimating the effects associated
with a measured subset of limiting factors is to account for the effects of unmeasured factors in an ecologically realistic
matter. We used quantile regression to elucidate multiple stressor effects on end-of-season biomass data from two salt marsh
sites in coastal Louisiana collected for 18 yr. Stressor effects evaluated based on available data were flooding, salinity
air temperature, cloud cover, precipitation deficit, grazing by muskrat, and surface water nitrogen and phosphorus. Precipitation
deficit combined with surface water nitrogen provided the best two-parameter model to explain variation in the peak biomass
with different slopes and intercepts for the two study sites. Precipitation deficit, cloud cover, and temperature were significantly
correlated with each other. Surface water nitrogen was significantly correlated with surface water phosphorus and muskrat
density. The site with the larger duration of flooding showed reduced peak biomass, when cloud cover and surface water nitrogen
were optimal. Variation in the relatively low salinity occurring in our study area did not explain any of the variation inSpartina alterniflora biomass. 相似文献
5.
Low-low satellite-to-satellite tracking: a comparison between analytical linear orbit perturbation theory and numerical integration 总被引:1,自引:0,他引:1
P.N.A.M. Visser 《Journal of Geodesy》2005,79(1-3):160-166
Low-low satellite-to-satellite tracking (ll-SST) range-rate observations have been predicted by two methods: one based on a linear perturbation theory in combination with the Hill equations, and one based on solving the equations of motion of two low-flying satellites by numerical integration. The two methods produce almost equivalent Fourier spectra of the range-rate observations after properly taking into account a few resonant terms. For a typical GRACE-type configuration, where the two satellites trail each other at a distance of 300 km at an altitude of 460 km, and in the presence of the EGM96 gravity field model, complete to spherical harmonic degree and order 70, the agreement between the Fourier spectra is about 1 mm/s compared to a root-mean-square (RMS) value of more than 220 mm/s for the range-rate signal. The discrepancy of 1 mm/s can be reduced significantly when not taking into account perturbations caused by the J2 term. Excluding the J2 term, the agreement between the two methods improves to 0.4 mm/s compared to a RMS value of 6 mm/s for the range-rate signal. These values are 0.01 and 2.3 mm/s when ignoring the spectrum for frequencies below two cycles per orbital revolution, reducing the discrepancy even further to about 0.5% of the signal. The selected linear perturbation theory is thus capable of modeling gravity field induced range-rate observations with very high precision for a large part of the spectrum. 相似文献
6.
Basins within the African sector of Gondwana contain a Late Palaeozoic to Early Mesozoic Gondwana sequence unconformably
overlying Precambrian basement in the interior and mid-Palaeozoic strata along the palaeo-Pacific margin. Small sea-board
Pacific basins form an exception in having a Carboniferous to Early Permian fill overlying Devonian metasediments and intrusives.
The Late Palaeozoic geographic and tectonic changes in the region followed four well-defined consecutive events which can
also be traced outside the study area. During the Late Devonian to Early Carboniferous period (up to 330 Ma) accretion of
microplates along the Patagonian margin of Gondwana resulted in the evolution of the Pacific basins. Thermal uplift of the
Gondwana crust and extensive erosion causing a break in the stratigraphic record characterised the period between 300 and
330 Ma. At the end of this period the Gondwana Ice Sheet was well established over the uplands. The period 260–300 Ma evidenced
the release of the Gondwana heat and thermal subsidence caused widespread basin formation. Late Carboniferous transpressive
strike-slip basins (e.g. Sierra Australes/Colorado, Karoo-Falklands, Ellsworth-Central Transantarctic Mountains) in which
thick glacial deposits accumulated, formed inboard of the palaeo-Pacific margin. In the continental interior the formation
of Zambesi-type rift and extensional strike-slip basins were controlled by large mega-shear systems, whereas rare intracratonic
thermal subsidence basins formed locally. In the Late Permian the tectonic regime changed to compressional largely due to
northwest-directed subduction along the palaeo-Pacific margin. The orogenic cycle between 240 and 260 Ma resulted in the formation
of the Gondwana fold belt and overall north–south crustal shortening with strike-slip motions and regional uplift within the
interior. The Gondwana fold belt developed along a probable weak crustal zone wedged in between the cratons and an overthickened
marginal crustal belt subject to dextral transpressive motions. Associated with the orogenic cycle was the formation of mega-shear
systems one of which (Falklands-East Africa-Tethys shear) split the supercontinent in the Permo-Triassic into a West and an
East Gondwana. By a slight clockwise rotation of East Gondwana a supradetachment basin formed along the Tethyan margin and
northward displacement of Madagascar, West Falkland and the Gondwana fold belt occurred relative to a southward motion of
Africa.
Received: 2 October 1995 / Accepted: 28 May 1996 相似文献
7.
Researching the South African tourism and development nexus 总被引:1,自引:0,他引:1
Under apartheid, tourism was essentially anti-developmental in focus and tourism research largely centred on the recreation
of white South Africans. Since the 1994 democratic transition tourism has become recognised as an increasingly important sector
for South Africa's economy and for achieving the government's goals for reconstruction and development. In this paper a review
and critical analysis is presented of the changing directions and foci of tourism scholarship in South Africa. It is shown
that South African scholars are beginning to respond to the challenges posed by tourism as a developmental focus.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
8.
Lyu Chuangxin Nishimura Satoshi Amiri Seyed Ali Ghoreishian Zhu Feng Eiksund Gudmund Reidar Grimstad Gustav 《Acta Geotechnica》2021,16(12):3831-3847
Acta Geotechnica - A systematical testing program on frozen Onsøy clay under isotropic loading and undrained shearing at different temperatures... 相似文献
9.
Samuel Ebert Addi Bischoff Dennis Harries Sarah Lentfort Jean‐Alix Barrat Andreas Pack Jrme Gattacceca Robbin Visser Peter Schmid‐Beurmann Stephan Kimpel 《Meteoritics & planetary science》2019,54(2):328-356
Based on the high abundance of fine‐grained material and its dark appearance, NWA 11024 was recognized as a CM chondrite, which is also confirmed by oxygen isotope measurements. But contrary to known CM chondrites, the typical phases indicating aqueous alteration (e.g., phyllosilicates, carbonates) are missing. Using multiple analytical techniques, this study reveals the differences and similarities to known CM chondrites and will discuss the possibility that NWA 11024 is the first type 3 CM chondrite. During the investigation, two texturally apparent tochilinite–cronstedtite intergrowths were identified within two thin sections. However, the former phyllosilicates were recrystallized to Fe‐rich olivine during a heating event without changing the textural appearance. A peak temperature of 400–600 °C is estimated, which is not high enough to destroy or recrystallize calcite grains. Thus, calcites were never constituents of the mineral paragenesis. Another remarkable feature of NWA 11024 is the occurrence of unknown clot‐like inclusions (UCLIs) within fine‐grained rims, which are unique in this clarity. Their density and S concentration are significantly higher than of the surrounding fine‐grained rim and UCLIs can be seen as primary objects that were not formed by secondary alteration processes inside the rims. Similarities to chondritic and cometary interplanetary dust particles suggest an ice‐rich first‐generation planetesimal for their origin. In the earliest evolution, NWA 11024 experienced the lowest degree of aqueous alteration of all known CM chondrites and subsequently, a heating event dehydrated the sample. We suggest to classify the meteorite NWA 11024 as the first type 3 CM chondrite similar to the classification of CV3 chondrites (like Allende) that could also have lost their matrix phyllosilicates by thermal dehydration. 相似文献
10.
Johannes Bouman Sietse Rispens Thomas Gruber Radboud Koop Ernst Schrama Pieter Visser Carl Christian Tscherning Martin Veicherts 《Journal of Geodesy》2009,83(7):659-678
One of the products derived from the gravity field and steady-state ocean circulation explorer (GOCE) observations are the
gravity gradients. These gravity gradients are provided in the gradiometer reference frame (GRF) and are calibrated in-flight
using satellite shaking and star sensor data. To use these gravity gradients for application in Earth scienes and gravity
field analysis, additional preprocessing needs to be done, including corrections for temporal gravity field signals to isolate
the static gravity field part, screening for outliers, calibration by comparison with existing external gravity field information
and error assessment. The temporal gravity gradient corrections consist of tidal and nontidal corrections. These are all generally
below the gravity gradient error level, which is predicted to show a 1/f behaviour for low frequencies. In the outlier detection, the 1/f error is compensated for by subtracting a local median from the data, while the data error is assessed using the median absolute
deviation. The local median acts as a high-pass filter and it is robust as is the median absolute deviation. Three different
methods have been implemented for the calibration of the gravity gradients. All three methods use a high-pass filter to compensate
for the 1/f gravity gradient error. The baseline method uses state-of-the-art global gravity field models and the most accurate results
are obtained if star sensor misalignments are estimated along with the calibration parameters. A second calibration method
uses GOCE GPS data to estimate a low-degree gravity field model as well as gravity gradient scale factors. Both methods allow
to estimate gravity gradient scale factors down to the 10−3 level. The third calibration method uses high accurate terrestrial gravity data in selected regions to validate the gravity
gradient scale factors, focussing on the measurement band. Gravity gradient scale factors may be estimated down to the 10−2 level with this method. 相似文献