Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus locusta

The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 microgCu l(-1) on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP: thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P < 0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P < 0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within I day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P < 0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.     

Bioenhancement of cadmium transfer along a multi-level food chain

Previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (TAM) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (HSP - e.g., metallothioneins). The aim of the current investigation was to determine the influence of TAM on the trophic transfer of Cd along an experimental, three-level food chain: Artemia franciscana (brine shrimp)-->Palaemonetes pugio (grass shrimp)-->Fundulus heteroclitus (mummichog). P. pugio were fed for 7 days on A. franciscana exposed to Cd in solution (including (109)Cd as radiotracer) and subjected to subcellular fractionation or fed to F. heteroclitus. An HSP-driven increase in the percentage of Cd associated with TAM (TAM-Cd%) in A. franciscana exposed to 1 muM Cd resulted in a bioenhancement (i.e., a greater than linear increase with respect to A. franciscana exposure) of Cd trophic transfer to P. pugio. Increased dietary Cd exposure did not affect TAM-Cd% in P. pugio nor trophic transfer to F. heteroclitus.     

用快速仿真退火进行走时层析成像

本文主要介绍一种与射线追踪无关的走时成像方法，使用一种非线性最优化算法，称为非常快速的仿真退火。利用立方Ｂ样条对地下地质体进行参数化，此网格在水平和垂直坐标是可变化的，这个灵活的网格可以容 等复杂程度的构造，并只用几个节点，我们试图求观测与预测走时之间的均言差进行极小化。时使用有限差分算法，不仅在样条节点位置上求速度，而且还求在每个维方向上的网格间距，由于在计算期间避免了局部极小，求出的解与初始模     

Uniform head in horizontal and vertical wells

The steady-state head within a fully penetrating well may be estimated by evaluating the Thiem equation at the radius of the well. A method is presented here to extend results from the Thiem equation to horizontal wells and to partially penetrating wells. The particular model used in this investigation is based upon the analytic element method; it accurately reproduces a boundary condition of uniform head along the cylindrical surface at the perforated face of the well. This model is exercised over a representative range of parameters including the well's length, radius, and pumping rate, and the aquifer's hydraulic conductivity and thickness. Results are presented in a set of figures and tables that compare the well's drawdown to the drawdown that would have been obtained using the Thiem solution with the same pumping rate and radius. A methodology is presented to estimate the head within a horizontal or partially penetrating well by adding a correction term to results that can be readily obtained from computer models of vertical fully penetrating wells. This approach may also be used to contrast the differences in head between horizontal and vertical wells of various lengths, radii, and placement elevations.     

基于光谱信息的遥感图像空间域自适应滤波

遥感图像往往由于内外因素的干扰含有各种噪声 ,为提高图像质量人们对其进行滤波。常规的滤波方式完全基于图像空间域 ,在消除异常点使图像平滑的同时使边界变得不清楚从而使图像模糊 ;或者正相反 ,在边缘信息得到增强时也放大了图像噪声而使图像变得粗糙。该文的自适应滤波方法充分利用多光谱和高光谱遥感图像的光谱维信息 ,滤波时不仅要使均匀的地块得到平滑 ,而且要使地块间的边缘、局部的异常点、线仍然得到保存、在光滑的同时尽量保持原有的数值特征     

Integration of biochemical, histochemical and toxicogenomic indices for the assessment of health status of mussels from the Tamar Estuary, U.K

The aim of this study was to examine whether a combination of biochemical, histopathological and toxicogenomic data could be used as a valuable tool for the assessment of biological risk associated with pollutants within the Tamar River and Estuary, S.W. England, U.K. Accordingly, biochemical and histopathological biomarkers (protein carbonyls, lipofuscin, neutral lipids, lysosomal stability [N-acetyl-β-hexosaminidase and neutral red], lysosomal volume, ferric reducing antioxidant power [FRAP] and malonaldehyde [MDA]) and gene expression profiles were assessed in 5 sites from the Tamar River and Estuary (Neal Point, Town Quay, Wilcove, Cremyll Ferry and Whitsand; and a reference site, Trebarwith Strand, N. Cornwall). PAHs were measured in mussel tissue and sediment and metals were measured in mussel tissue only. Data from the biomarkers was integrated into a Mussel Expert System (MES) model to produce a simple assessment of mussel stress. Clear gradients of mussel toxicity were identified by the biomarkers (with the exception of neutral lipids) with the highest impacted animals found furthest up the Tamar, whilst the MES was unable to identify a gradient of effect. Gene expression profiles also indicated a gradient of stress with the greatest number of significantly up- or down- regulated genes found at the uppermost 2 sites. The MES did, however, determine that mussels from all sites, except the reference site, were highly stressed; a conclusion that could not be inferred from the biomarker data alone. It is concluded that the MES is a valuable tool that permits integration and interpretation of complex sets of biomarker data by identifying the biological meaning of biomarker changes.     

基于偏振原理的水面反射光的剥离

水面的镜面反射是影响水环境遥感的关键因素之一。通过分析气-水界面反射光的偏振特性,从多角度偏振遥感的角度,提出一种剥离水面反射光的方法,并通过室内实验进行了验证。将ASD光谱仪与线偏振片结合扩展成高光谱偏振传感器,获取不同观测天顶角时水面反射光的偏振光谱。结果表明,当探测角不满足布儒斯特角时,在偏振片的主消光方向能剥离绝大部分的水面反射光;当入射角越接近布儒斯特角,其剥离效果越佳;以布儒斯特角观测时,可以达到完全剥离的效果。即该方法能显著削弱、甚至完全剥离水面的反射光,直接获取离水辐亮度,对于提高水色遥感的精度有着重要的应用价值。     

利用形状函数解算体积

在工程测量中，经常需要解算各种形状的物体体积。本文介绍了一种求解体积的数值算法，即用一个数学单元近似表述物体的形状，然后再通过数值积分解算体积。定义数学单元时，要求它们必须经过物体表面边界上预先定义的８个或者２０个结点。实现该算法的计算机程序只需要一个简单的数据文件，其中包括结点坐标和结点个数。文中还给出了几个典型问题的解算结果，其精度可精确到３％，较好地满足了限差要求。