Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity

We have previously demonstrated that medaka CYP3A is associated with metabolism of several endobiotics including steroids and bile acids. In this study, we demonstrate that medaka CYP3A catalysis exhibits unusual kinetic behaviors consistent with allosteric interaction which cannot be described by hyperbolic kinetic models. Using 7-benzyloxy-4-(trifluoromethyl)-coumarin (BFC) and nonylphenol as CYP3A substrates, we describe both homotropic and heterotropic cooperative interactions. Given the role of CYP3A in maintaining the homeostatic balance for numerous endobiotics, enzymatic activation/inhibition by endocrine disruptors (EDCs) represents a putative (non-genomic) mechanism for endocrine disruption.     

边缘检测在D-InSAR变形图中提取地表断裂带的应用

利用Doris软件对伊朗Bam地震ENVISAT图像进行处理,得到InSAR差分干涉图并对InSAR差分干涉图进行了相位解缠;然后用几种边缘检测算法对解缠后的差分图进行边缘检测以提取地面断裂带的位置,最后对这几种边缘检测算法的检测结果进行了评价.     

火山热液型铅锌矿床岩石地球化学特征及预测指标

火山热液型铅锌矿床赋矿围岩、后期脉岩和蚀变岩石中成矿元素铅锌银含量高,矿体元素组合复杂,原生异常发育,并存在着原生分带现象。矿体前缘元素为I、Hg、As、Sb、B、Ba;近矿指示元素是Pb、Zn、Ag、Au、Cd、Mn、Cu;尾部或矿下指示元素为Cu、Mo、W、Sn。根据矿床指示元素的分布规律和原生晕的分带性,研制了判别矿床剥蚀程度和评价异常的地球化学指标,建立了该类矿床的地球化学找矿预测标志。     

邹平火山岩盆地铜矿地质成因地球物理特征探讨

邹平铜矿处于齐河-广饶深大断裂带南部的邹平火山岩盆地中,形成于破火山口火山通道充填的石英正长闪长岩岩颈中央上部,包括伟晶岩型铜矿和细脉浸染状斑铜矿床两种类型。前者矿体较小,但品位高;后者品位较低,但规模中等。含矿石英正长闪长岩等密度小、磁性弱,故在火山岩系中呈现高背景重力场上的重力低和杂乱高磁场背景中的低负异常,即“重磁同低”,且高极化。重磁同低异常区和高极化率异常带,是本区寻找铜矿的有利部位。     

抗冰救灾集结号吹响之后

2月15日．当人们尚沉浸在年味儿未尽的喜庆氛围时,位于九江市的二六七大队接到了市政府应急管理办的紧急通知：根据江西省政府指示,派员支援兄弟地市电力抢修工作。     

Biogeochemical cycles of carbon, sulfur, and free oxygen in a microbial mat

Complete budgets for carbon and oxygen have been constructed for cyanobacterial mats dominated by Microcoleus chthonoplastes from the evaporating ponds of a salt works located in Guerrero Negro, Baja California Sur, Mexico. Included in the budget are measured rates of O2 production, sulfate reduction, and elemental exchange across the mat/brine interface, day and night, at various temperatures and times of the year. We infer from this data the various sinks for O2, as well as the sources of carbon for primary production. To summarize, although seasonal variability exists, a major percentage of the O2 produced during the day did not diffuse out of the mat but was used within the mat to oxidize both organic carbon and the sulfide produced by sulfate reduction. At night, most of the O2 that diffused into the mat was used to oxidize sulfide, with O2 respiration of minor importance. During the day, the internal mat processes of sulfate reduction and O2 respiration generated as much or more inorganic carbon (DIC) for primary production as diffusion into the mat. Also, oxygenic photosynthesis was the most important process of carbon fixation, although anoxygenic photosynthesis may have been important at low light levels during some times of the year. At night, the DIC lost from the mat was mostly from sulfate reduction. Elemental fluxes across the mat/brine interface indicated that carbon with an oxidation state of greater than zero was taken up by the mat during the day and liberated from the mat at night. Overall, carbon with an average oxidation state of near zero accumulated in the mat. Both carbon fixation and carbon oxidation rates varied with temperature by a similar amount. These mats are thus closely coupled systems where rapid rates of photosynthesis both require and fuel rapid rates of heterotrophic carbon oxidation.     

Sulfur isotope fractionation during bacterial sulfate reduction in organic-rich sediments

Isotope fractionation during sulfate reduction by natural populations of sulfate-reducing bacteria was investigated in the cyanobacterial microbial mats of Solar Lake, Sinai and the sediments of Logten Lagoon sulfuretum, Denmark. Fractionation was measured at different sediment depths, sulfate concentrations, and incubation temperatures. Rates of sulfate reduction varied between 0.1 and 37 micromoles cm-3 d-1, with the highest rates among the highest ever reported from natural sediments. The depletion of 34S during dissimilatory sulfate reduction ranged from 16% to 42%, with the largest 34S-depletions associated with the lowest rates of sulfate reduction and the lowest 34S-depletions with the highest rates. However, at high sulfate reduction rates (>10 micromoles cm-3 d-1) the lowest fractionation was 20% independent of the rates. Overall, there was a similarity between the fractionation obtained by the natural populations of sulfate reducers and previous measurements from pure cultures. This was somewhat surprising given the extremely high rates of sulfate reduction in the experiments. Our results are explained if we conclude that the fractionation was mainly controlled by the specific rate of sulfate reduction (mass cell-1 time-1) and not by the absolute rate (mass volume-1 time-1). Sedimentary sulfides (mainly FeS2) were on average 40% depleted in 34S compared to seawater sulfate. This amount of depletion was more than could be explained by the isotopic fractionations that we measured during bacterial sulfate reduction. Therefore, additional processes contributing to the fractionation of sulfur isotopes in the sediments are indicated. From both Solar Lake and Logten Lagoon we were able to enrich cultures of elemental sulfur-disproportionating bacteria. We suggest that isotope fractionation accompanying elemental sulfur disproportionation contributes to the 34S depletion of sedimentary sulfides at our study sites.