Temperature and its control of isotope fractionation by a sulfate-reducing bacterium

A synthesis of previous results, which we dub the “standard model,” provides a prediction as to how isotope fractionation during sulfate reduction should respond to physiological variables such as specific rate of sulfate reduction and environmental variables such as substrate availability and temperature. The standard model suggests that isotope fractionation should decrease with increasing specific rates of sulfate reduction (rate per cell). Furthermore, the standard model predicts that low fractionations should be found at both high and low temperatures whereas the highest fractionations should be found in the intermediate temperature range. These fractionation trends are controlled, as a function of temperature, by the balance between the transfer rates of sulfate into and out of the cell and the exchange between the sulfur pools internal to the organism. We test this standard model by conducting experiments on the growth physiology and isotope fractionation, as a function of temperature, by the sulfate-reducing bacterium Desulfovibrio desulfuricans (DSMZ 642). Our results contrast with the “standard model” by showing a positive correlation between specific rates of sulfate reduction and fractionation. Also by contrast with the standard model, we found the highest fractionations at low and high temperatures and the lowest fractionations in the intermediate temperature range. We develop a fractionation model which can be used to explain both our results as well as the results of the “standard model.” Differences in fractionation with temperature relate to differences in the specific temperature response of internal enzyme kinetics as well as the exchange rates of sulfate in and out of the cell. It is expected that the kinetics of these processes will show strain-specific differences.     

Map-Based Exploratory Evaluation of Non-Medical Determinants of Population Health

Multi‐criteria evaluation (MCE) and decision‐making are increasingly combined with interactive tools to assist users with visual thinking and exploring decision strategies. The interactive control of criterion combination rules and the simultaneous observation of geographic space and criterion space provide a means of investigating the sensitivity of the decision outcome to the decision‐maker's preferences. The Analytic Hierarchy Process (AHP) is an MCE method that has been successfully implemented in management processes including those addressed by Geographic Information Systems. In this paper, we present a map‐based, interactive AHP implementation, which provides a link between a well‐understood decision support method and exploratory geographic visualization. Using a case study with public health data for the Province of Ontario, Canada, we demonstrate that exploratory map use increases the effectiveness of the AHP‐based evaluation of population health.     

An Open,Object-Based Framework for Generating Anisotropy in Sedimentary Subsurface Models

The spatial distribution of hydraulic properties in the subsurface controls groundwater flow and solute transport. However, many approaches to modeling these distributions do not produce geologically realistic results and/or do not model the anisotropy of hydraulic conductivity caused by bedding structures in sedimentary deposits. We have developed a flexible object-based package for simulating hydraulic properties in the subsurface—the Hydrogeological Virtual Realities (HyVR) simulation package. This implements a hierarchical modeling framework that takes into account geological rules about stratigraphic bounding surfaces and the geometry of specific sedimentary structures to generate realistic aquifer models, including full hydraulic-conductivity tensors. The HyVR simulation package can create outputs suitable for standard groundwater modeling tools (e.g., MODFLOW), is written in Python, an open-source programming language, and is openly available at an online repository. This paper presents an overview of the underlying modeling principles and computational methods, as well as an example simulation based on the Macrodispersion Experiment site in Columbus, Mississippi. Our simulation package can currently simulate porous media that mimic geological conceptual models in fluvial depositional environments, and that include fine-scale heterogeneity in distributed hydraulic parameter fields. The simulation results allow qualitative geological conceptual models to be converted into digital subsurface models that can be used in quantitative numerical flow-and-transport simulations, with the aim of improving our understanding of the influence of geological realism on groundwater flow and solute transport.     

Pyroclastic Flow Hazard at Volcán Citlaltépetl

Volcán Citlaltépetl (Pico de Orizaba) with an elevation of 5,675 m is the highest volcano in North America. Its most recent catastrophic events involved the production of pyroclastic flows that erupted approximately 4,000, 8,500, and 13,000 years ago. The distribution of mapped deposits from these eruptions gives an approximate guide to the extent of products from potential future eruptions. Because the topography of this volcano is constantly changing computer simulations were made on the present topography using three computer algorithms: energy cone, FLOW2D, and FLOW3D. The Heim Coefficient (), used as a code parameter for frictional sliding in all our algorithms, is the ratio of the assumed drop in elevation (H) divided by the lateral extent of the mapped deposits (L). The viscosity parameter for the FLOW2D and FLOW3D codes was adjusted so that the paths of the flows mimicked those inferred from the mapped deposits. We modeled two categories of pyroclastic flows modeled for the level I and level II events. Level I pyroclastic flows correspond to small but more frequent block-and-ash flows that remain on the main cone. Level II flows correspond to more widespread flows from catastrophic eruptions with an approximate 4,000-year repose period. We developed hazard maps from simulations based on a National Imagery and Mapping Agency (NIMA) DTED-1 DEM with a 90 m grid and a vertical accuracy of ±30 m. Because realistic visualization is an important aid to understanding the risks related to volcanic hazards we present the DEM as modeled by FLOW3D. The model shows that the pyroclastic flows extend for much greater distances to the east of the volcano summit where the topographic relief is nearly 4,300 m. This study was used to plot hazard zones for pyroclastic flows in the official hazard map that was published recently.     

小型激光天文动力学空间计划概念

小型激光天文动力学空间计划是 :使用在太阳轨道上无拖曳航天器和地面站以激光干涉和脉冲测距的方法 ,精确地探讨天文动力学 ,检测相对论与时空基本定律 ,改进探测引力波的灵敏度以及更准确地测定太阳、行星和小行星的参数。 1 969年开始的月球激光 (反射 )测距 ,对地球物理、参考坐标的选定、相对论的检验均有重要的贡献。 3 0年来 ,激光技术的长足进步 ,使现在正是适合于开始进行研究空间有源 (主动 )测距和光波空间通讯的时候。激光天文动力学的兴起是必然的趋势 ,其精确度将比现在提高 3到 6个数量级 ,将是天文动力学革命性的发展。小型激光天文动力学空间计划可以起到带头作用。它的关键技术有三 ,即 :弱光锁相、极精确无拖曳航天和高衰减日冕仪。弱光锁相已有长足的进步。对高衰减日冕仪的研究 ,也有了初步的方案。LISA空间计划将于 2 0 0 6年 8月发射SMART -2 ,研究测试极精确无拖曳航天。小型激光天文动力学空间计划的关键技术已日趋成熟。在第一届国际激光天文动力学研讨会 ( 2 0 0 1 ,9.1 3 -2 3 )中介绍了各相关学科背景及前沿研究 ,讨论了激光天文动力学空间计划科学目标及相关技术 ,并召开了两次小型激光天文动力学空间计划预研究筹备会 ,建立了和欧洲的合作关系。会后着手进行此项对基础     

The motion of a lunar satellite

Presented in this theory is a semianalytical solution for the problem of the motion of a satellite in orbit around the moon. The principal perturbations on such a body are due to the nonspherical gravity field of the moon, the attraction of the earth, and, to a lesser degree, the attraction of the sun. The major part of the problem is solved by means of the celebrated von Zeipel Method, first successfully applied to the motion of an artificial earth satellite by Brouwer in 1959. After eliminating from the Hamiltonian all terms with the period of the satellite and those with the period of the moon, it is suggested to solve the remaining problem with the aid of numerical integration of the modified equations of motion.This theory was written in 1964 and presented as a dissertation to Yale University in 1965. Since then a great deal has been learned about the gravity field of the moon. It seems that quite a number of recently determined gravity coefficients would qualify as small quantities of order two. Hence, according to the truncation criteria employed, they should be considered in the present theory. However, the author has not endeavored to update the work accordingly. The final results, therefore, are incomplete in the lunar gravitational perturbations. Nevertheless, the theory does give the largest such variations and it does present the methods by which perturbations may be derived for any gravity terms not actually developed.     

The 1s 2s states of negative hydrogen

Term values for the 1s 2s states of H^– are calculated with the use of simple eigenfunction approximations constructed on the basis of available Hartree-type functions for the 1s ² and 2s ² states of the ion. The results seem to support a tentative identification of the interstellar diffuse absorption bands at 4890 and 6180 as due to negative hydrogen.     

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

Role of time variant and invariant terms on soil moisture spatio-temporal variability in a subhumid coastal wetland

The knowledge of soil moisture spatio-temporal variability is highly relevant for water resources management. This paper reports an analysis of the spatial–temporal variability of soil moisture data for a small to medium-scale soil-sensors network in a coastal wetland of southwestern Spain. Measurements were taken from five sites located in the Doñana National Park over the time-period of one hydrological year from September 2017 to September 2018. The total area of the soil-sensors network shows an extension about 25 × 3 km. Soil moisture data was separated into time invariant (the temporal mean of the whole period at each site) and time-variant terms (the deviations of soil moisture from the mean, or anomalies). The time-invariant component was generally the main contributor to the total spatial variance of soil moisture and it was mostly controlled by the groundwater levels in the area. Nevertheless, the time variant terms have a huge effect on soil moisture variability in very dry states. Characteristic convex time-dependent patterns for this field site were found between spatially averaged soil moisture and its variability. This information could be used for the up and downscaling of soil moisture from satellite data. Those patterns of relation between spatial mean and variability of soil moisture were only affected by heavy rainfalls giving rise to hysteretic behaviour. This study shows that even though groundwater level is a time-variant variable, it significantly affects soil moisture's time-variant but also time-invariant terms due to the different average groundwater level depths at the different sites.